TY - JOUR A1 - Kramer, Rolf A. A1 - Kainmüller, Eva K. A1 - Flehr, Roman A1 - Kumke, Michael Uwe A1 - Bannwarth, Willi T1 - Quenching of the long-lived Ru(II)bathophenanthroline luminescence for the detection of supramolecular interactions Y1 - 2008 ER - TY - JOUR A1 - Kramer, Rolf A. A1 - Flehr, Roman A1 - Lay, Myriam A1 - Kumke, Michael Uwe A1 - Bannwarth, Willi T1 - Comparative studies of different quinoline derivatives as donors in fluorescence-resonance-energy-transfer (FRET) : systems in combination with a (Bathophenanthroline)ruthenium(II) complex as acceptor Y1 - 2009 UR - http://www3.interscience.wiley.com/cgi-bin/jhome/5007133/ U6 - https://doi.org/10.1002/hlca.200900235 SN - 0018-019X ER - TY - JOUR A1 - Kienzler, Andrea A1 - Flehr, Roman A1 - Kramer, Rolf A. A1 - Gehne, Soeren A1 - Kumke, Michael Uwe A1 - Bannwarth, Willi T1 - Novel Three-Color FRET Tool Box for Advanced Protein and DNA Analysis JF - Bioconjugate chemistry N2 - We report on a new three-color FRET system which we were able to verify in peptides as well as in synthetic DNA. All three chromophores could be introduced by a building block approach avoiding postsynthetic labeling. Additional features are robustness, matching spectroscopic properties, high-energy transfer, and sensitivity. The system was investigated in detail on a set of peptides as well as an array of tailored oligonucleotides. The detailed analysis of the experimental data and comparison with theoretical considerations were in excellent agreement. It is shown that in the case of polypeptides specific interaction with the fluorescence probes has to be considered. In contrast with DNA, the fluorescence probes did not show any indications of such interactions. The novel three-color FRET toolbox revealed the potential for applications studying fundamental processes of three interacting molecules in life science applications. Y1 - 2011 U6 - https://doi.org/10.1021/bc2002659 SN - 1043-1802 VL - 22 IS - 9 SP - 1852 EP - 1863 PB - American Chemical Society CY - Washington ER -