TY  - JOUR
A1  - Dey, Pradip
A1  - Adamovski, Miriam
A1  - Friebe, Simon
A1  - Badalyan, Artavazd
A1  - Mutihac, Radu-Cristian
A1  - Paulus, Florian
A1  - Leimkühler, Silke
A1  - Wollenberger, Ursula
A1  - Haag, Rainer
T1  - Dendritic polyglycerol-poly(ethylene glycol)-based polymer networks for biosensing application
JF  - ACS applied materials & interfaces
N2  - This work describes the formation of a new dendritic polyglycerol-poly(ethylene glycol)-based 3D polymer network as a matrix for immobilization of the redox enzyme periplasmatic aldehyde oxidoreductase to create an electrochemical biosensor. The novel network is built directly on the gold surface, where it simultaneously stabilizes the enzyme for up to 4 days. The prepared biosensors can be used for amperometric detection of benzaldehyde in the range of 0.8-400 mu M.
KW  - biosensors
KW  - hydrogel
KW  - amperometry
KW  - dendritic
Y1  - 2014
U6  - https://doi.org/10.1021/am502018x
SN  - 1944-8244
VL  - 6
IS  - 12
SP  - 8937
EP  - 8941
PB  - American Chemical Society
CY  - Washington
ER  - 
TY  - JOUR
A1  - Dey, Pradip
A1  - Bergmann, Tobias
A1  - Cuellar-Camacho, Jose Luis
A1  - Ehrmann, Svenja
A1  - Chowdhury, Mohammad Suman
A1  - Zhang, Minze
A1  - Dahmani, Ismail
A1  - Haag, Rainer
A1  - Azad, Walid
T1  - Multivalent flexible nanogels exhibit broad-spectrum antiviral activity by blocking virus entry
JF  - ACS nano
N2  - The entry process of viruses into host cells is complex and involves stable but transient multivalent interactions with different cell surface receptors. The initial contact of several viruses begins with attachment to heparan sulfate (HS) proteoglycans on the cell surface, which results in a cascade of events that end up with virus entry. The development of antiviral agents based on multivalent interactions to shield virus particles and block initial interactions with cellular receptors has attracted attention in antiviral research. Here, we designed nanogels with different degrees of flexibility based on dendritic polyglycerol sulfate to mimic cellular HS. The designed nanogels are nontoxic and broad-spectrum, can multivalently interact with viral glycoproteins, shield virus surfaces, and efficiently block infection. We also visualized virus-nanogel interactions as well as the uptake of nanogels by the cells through clathrin-mediated endocytosis using confocal microscopy. As many human viruses attach to the cells through HS moieties, we introduce our flexible nanogels as robust inhibitors for these viruses.
KW  - multivalent
KW  - herpes simplex virus
KW  - heparan sulfate
KW  - nanoparticles
KW  - click chemistry
KW  - polyglycerol
Y1  - 2018
U6  - https://doi.org/10.1021/acsnano.8b01616
SN  - 1936-0851
SN  - 1936-086X
VL  - 12
IS  - 7
SP  - 6429
EP  - 6442
PB  - American Chemical Society
CY  - Washington
ER  - 
TY  - JOUR
A1  - Zabihi, Fatemeh
A1  - Graff, Patrick
A1  - Schumacher, Fabian
A1  - Kleuser, Burkhard
A1  - Hedtrich, Sarah
A1  - Haag, Rainer
T1  - Synthesis of poly(lactide-co-glycerol) as a biodegradable and biocompatible polymer with high loading capacity for dermal drug delivery
JF  - Nanoscale
N2  - Due to the low cutaneous bioavailability of tacrolimus (TAC), penetration enhancers are used to improve its penetration into the skin. However, poor loading capacity, non-biodegradability, toxicity, and in some cases inefficient skin penetration are challenging issues that hamper their applications for the dermal TAC delivery. Here we present poly(lactide-co-glycerol) (PLG) as a water soluble, biodegradable, and biocompatible TAC-carrier with high loading capacity (14.5% w/w for TAC) and high drug delivery efficiencies into the skin. PLG was synthesized by cationic ring-opening copolymerization of a mixture of glycidol and lactide and showed 35 nm and 300 nm average sizes in aqueous solutions before and after loading of TAC, respectively. Delivery experiments on human skin, quantified by fluorescence microscopy and LC-MS/MS, showed a high ability for PLG to deposit Nile red and TAC into the stratum corneum and viable epidermis of skin in comparison with Protopic (R) (0.03% w/w, TAC ointment). The cutaneous distribution profile of delivered TAC proved that 80%, 16%, and 4% of the cutaneous drug level was deposited in the stratum corneum, viable epidermis, and upper dermis, respectively. TAC delivered by PLG was able to efficiently decrease the IL-2 and TSLP expressions in human skin models. Taking advantage of the excellent physicochemical and biological properties of PLG, it can be used for efficient dermal TAC delivery and potential treatment of inflammatory skin diseases.
Y1  - 2018
U6  - https://doi.org/10.1039/c8nr05536j
SN  - 2040-3364
SN  - 2040-3372
VL  - 10
IS  - 35
SP  - 16848
EP  - 16856
PB  - Royal Society of Chemistry
CY  - Cambridge
ER  -