TY  - JOUR
A1  - Zhou, Suqiong
A1  - Pan, Yuanwei
A1  - Zhang, Jianguang
A1  - Li, Yan
A1  - Neumann, Falko
A1  - Schwerdtle, Tanja
A1  - Li, Wenzhong
A1  - Haag, Rainer
T1  - Dendritic polyglycerol-conjugated gold nanostars with different densities of functional groups to regulate osteogenesis in human mesenchymal stem cells
JF  - Nanoscale
N2  - Nanomaterials play an important role in mimicking the biochemical and biophysical cues of the extracellular matrix in human mesenchymal stem cells (MSCs). Increasing studies have demonstrated the crucial impact of functional groups on MSCs, while limited research is available on how the functional group's density on nanoparticles regulates MSC behavior. Herein, the effects of dendritic polyglycerol (dPG)-conjugated gold nanostars (GNSs) with different densities of functional groups on the osteogenesis of MSCs are systematically investigated. dPG@GNS nanocomposites have good biocompatibility and the uptake by MSCs is in a functional group density-dependent manner. The osteogenic differentiation of MSCs is promoted by all dPG@GNS nanocomposites, in terms of alkaline phosphatase activity, calcium deposition, and expression of osteogenic protein and genes. Interestingly, the dPGOH@GNSs exhibit a slight upregulation in the expression of osteogenic markers, while the different charged densities of sulfate and amino groups show more efficacy in the promotion of osteogenesis. Meanwhile, the sulfated nanostars dPGS20@GNSs show the highest enhancement. Furthermore, various dPG@GNS nanocomposites exerted their effects by regulating the activation of Yes-associated protein (YAP) to affect osteogenic differentiation. These results indicate that dPG@GNS nanocomposites have functional group density-dependent influence on the osteogenesis of MSCs, which may provide a new insight into regulating stem cell fate.
Y1  - 2020
U6  - https://doi.org/10.1039/d0nr06570f
SN  - 2040-3364
SN  - 2040-3372
VL  - 12
IS  - 47
SP  - 24006
EP  - 24019
PB  - Royal Society of Chemistry
CY  - Cambridge
ER  -