TY  - JOUR
A1  - Yang, Lei
A1  - Perrera, Valentina
A1  - Saplaoura, Eleftheria
A1  - Apelt, Federico
A1  - Bahin, Mathieu
A1  - Kramdi, Amira
A1  - Olas, Justyna Jadwiga
A1  - Müller-Röber, Bernd
A1  - Sokolowska, Ewelina
A1  - Zhang, Wenna
A1  - Li, Runsheng
A1  - Pitzalis, Nicolas
A1  - Heinlein, Manfred
A1  - Zhang, Shoudong
A1  - Genovesio, Auguste
A1  - Colot, Vincent
A1  - Kragler, Friedrich
T1  - m(5)C Methylation Guides Systemic Transport of Messenger RNA over Graft Junctions in Plants
JF  - Current biology
N2  - In plants, transcripts move to distant body parts to potentially act as systemic signals regulating development and growth. Thousands of messenger RNAs (mRNAs) are transported across graft junctions via the phloem to distinct plant parts. Little is known regarding features, structural motifs, and potential base modifications of transported transcripts and how these may affect their mobility. We identified Arabidopsis thalianam RNAs harboring the modified base 5-methylcytosine (m(5)C) and found that these are significantly enriched in mRNAs previously described as mobile, moving over graft junctions to distinct plant parts. We confirm this finding with graft-mobile methylated mRNAs TRANSLATIONALLY CONTROLLED TUMOR PROTEIN 1 (TCTP1) and HEAT SHOCK COGNATE PROTEIN 70.1 (HSC70.1), whose mRNA transport is diminished in mutants deficient in m(5)C mRNA methylation. Together, our results point toward an essential role of cytosine methylation in systemic mRNA mobility in plants and that TCTP1 mRNA mobility is required for its signaling function.
Y1  - 2019
U6  - https://doi.org/10.1016/j.cub.2019.06.042
SN  - 0960-9822
SN  - 1879-0445
VL  - 29
IS  - 15
SP  - 2465
EP  - 2476.e5
PB  - Cell Press
CY  - Cambridge
ER  -