TY  - JOUR
A1  - Marcisz, Katarzyna
A1  - Jassey, Vincent E. J.
A1  - Kosakyan, Anush
A1  - Krashevska, Valentyna
A1  - Lahr, Daniel J. G.
A1  - Lara, Enrique
A1  - Lamentowicz, Lukasz
A1  - Lamentowicz, Mariusz
A1  - Macumber, Andrew
A1  - Mazei, Yuri
A1  - Mitchell, Edward A. D.
A1  - Nasser, Nawaf A.
A1  - Patterson, R. Timothy
A1  - Roe, Helen M.
A1  - Singer, David
A1  - Tsyganov, Andrey N.
A1  - Fournier, Bertrand
T1  - Testate amoeba functional traits and their use in paleoecology
JF  - Frontiers in Ecology and Evolution
N2  - This review provides a synthesis of current knowledge on the morphological and functional traits of testate amoebae, a polyphyletic group of protists commonly used as proxies of past hydrological changes in paleoecological investigations from peatland, lake sediment and soil archives. A trait-based approach to understanding testate amoebae ecology and paleoecology has gained in popularity in recent years, with research showing that morphological characteristics provide complementary information to the commonly used environmental inferences based on testate amoeba (morpho-)species data. We provide a broad overview of testate amoeba morphological and functional traits and trait-environment relationships in the context of ecology, evolution, genetics, biogeography, and paleoecology. As examples we report upon previous ecological and paleoecological studies that used trait-based approaches, and describe key testate amoebae traits that can be used to improve the interpretation of environmental studies. We also highlight knowledge gaps and speculate on potential future directions for the application of trait-based approaches in testate amoeba research.
KW  - protists
KW  - functional traits
KW  - morphological traits
KW  - ecology
KW  - peatlands
KW  - lakes
KW  - soils
KW  - trait-based approaches
Y1  - 2020
U6  - https://doi.org/10.3389/fevo.2020.575966
SN  - 2296-701X
VL  - 8
PB  - Frontiers Media
CY  - Lausanne
ER  - 
TY  - JOUR
A1  - Gutbier, Birgitt
A1  - Schönrock, Stefanie M.
A1  - Ehrler, Carolin
A1  - Haberberger, Rainer
A1  - Dietert, Kristina
A1  - Gruber, Achim D.
A1  - Kummer, Wolfgang
A1  - Michalick, Laura
A1  - Kuebler, Wolfgang M.
A1  - Hocke, Andreas C.
A1  - Szymanski, Kolja
A1  - Letsiou, Eleftheria
A1  - Lüth, Anja
A1  - Schumacher, Fabian
A1  - Kleuser, Burkhard
A1  - Mitchell, Timothy J.
A1  - Bertrams, Wilhelm
A1  - Schmeck, Bernd
A1  - Treue, Denise
A1  - Klauschen, Frederick
A1  - Bauer, Torsten T.
A1  - Tönnies, Mario
A1  - Weissmann, Norbert
A1  - Hippenstiel, Stefan
A1  - Suttorp, Norbert
A1  - Witzenrath, Martin
T1  - Sphingosine Kinase 1 Regulates Inflammation and Contributes to Acute Lung Injury in Pneumococcal Pneumonia via the Sphingosine-1-Phosphate Receptor 2
JF  - Critical care medicine
N2  - Objectives: Severe pneumonia may evoke acute lung injury, and sphingosine-1-phosphate is involved in the regulation of vascular permeability and immune responses. However, the role of sphingosine-1-phosphate and the sphingosine-1-phosphate producing sphingosine kinase 1 in pneumonia remains elusive. We examined the role of the sphingosine-1-phosphate system in regulating pulmonary vascular barrier function in bacterial pneumonia. Design: Controlled, in vitro, ex vivo, and in vivo laboratory study. Subjects: Female wild-type and SphK1-deficient mice, 8-10 weeks old. Human postmortem lung tissue, human blood-derived macrophages, and pulmonary microvascular endothelial cells. Interventions: Wild-type and SphK1-deficient mice were infected with Streptococcus pneumoniae. Pulmonary sphingosine-1-phosphate levels, messenger RNA expression, and permeability as well as lung morphology were analyzed. Human blood-derived macrophages and human pulmonary microvascular endothelial cells were infected with S. pneumoniae. Transcellular electrical resistance of human pulmonary microvascular endothelial cell monolayers was examined. Further, permeability of murine isolated perfused lungs was determined following exposition to sphingosine-1-phosphate and pneumolysin. Measurements and Main Results: Following S. pneumoniae infection, murine pulmonary sphingosine-1-phosphate levels and sphingosine kinase 1 and sphingosine-1-phosphate receptor 2 expression were increased. Pneumonia-induced lung hyperpermeability was reduced in SphK1(-/-) mice compared with wild-type mice. Expression of sphingosine kinase 1 in macrophages recruited to inflamed lung areas in pneumonia was observed in murine and human lungs. S. pneumoniae induced the sphingosine kinase 1/sphingosine-1-phosphate system in blood-derived macrophages and enhanced sphingosine-1-phosphate receptor 2 expression in human pulmonary microvascular endothelial cell in vitro. In isolated mouse lungs, pneumolysin-induced hyperpermeability was dose dependently and synergistically increased by sphingosine-1-phosphate. This sphingosine-1-phosphate-induced increase was reduced by inhibition of sphingosine-1-phosphate receptor 2 or its downstream effector Rho-kinase. Conclusions: Our data suggest that targeting the sphingosine kinase 1-/sphingosine-1-phosphate-/sphingosine-1-phosphate receptor 2-signaling pathway in the lung may provide a novel therapeutic perspective in pneumococcal pneumonia for prevention of acute lung injury.
KW  - acute lung injury
KW  - pneumococcal pneumonia
KW  - sphingosine kinase 1
KW  - sphingosine-1-phosphate
KW  - sphingosine-1-phosphate receptor 2
Y1  - 2018
U6  - https://doi.org/10.1097/CCM.0000000000002916
SN  - 0090-3493
SN  - 1530-0293
VL  - 46
IS  - 3
SP  - e258
EP  - e267
PB  - Lippincott Williams & Wilkins
CY  - Philadelphia
ER  -