TY  - JOUR
A1  - King, Turi E.
A1  - Gonzalez-Fortes, Gloria M.
A1  - Balaresque, Patricia
A1  - Thomas, Mark G.
A1  - Balding, David
A1  - Delser, Pierpaolo Maisano
A1  - Neumann, Rita
A1  - Parson, Walther
A1  - Knapp, Michael
A1  - Walsh, Susan
A1  - Tonasso, Laure
A1  - Holt, John
A1  - Kayser, Manfred
A1  - Appleby, Jo
A1  - Forster, Peter
A1  - Ekserdjian, David
A1  - Hofreiter, Michael
A1  - Schuerer, Kevin
T1  - Identification of the remains of King Richard III
JF  - Nature Communications
Y1  - 2014
U6  - https://doi.org/10.1038/ncomms6631
SN  - 2041-1723
VL  - 5
PB  - Nature Publ. Group
CY  - London
ER  - 
TY  - JOUR
A1  - Scheller, Frieder W.
A1  - Makower, Alexander
A1  - Ghindilis, A. L.
A1  - Bier, Frank Fabian
A1  - Ehrentreich-Förster, Eva
A1  - Wollenberger, Ursula
A1  - Bauer, Christian G.
A1  - Micheel, Burkhard
A1  - Pfeiffer, Dorothea
A1  - Szeponik, Jan
A1  - Michael, N.
A1  - Kaden, H.
T1  - Enzyme sensors for subnanomolar concentrations
Y1  - 1995
ER  - 
TY  - JOUR
A1  - Förster, Daniel W.
A1  - Bull, James K.
A1  - Lenz, Dorina
A1  - Autenrieth, Marijke
A1  - Paijmans, Johanna L. A.
A1  - Kraus, Robert H. S.
A1  - Nowak, Carsten
A1  - Bayerl, Helmut
A1  - Kühn, Ralph
A1  - Saveljev, Alexander P.
A1  - Sindicic, Magda
A1  - Hofreiter, Michael
A1  - Schmidt, Krzysztof
A1  - Fickel, Jörns
T1  - Targeted resequencing of coding DNA sequences for SNP discovery in nonmodel species
JF  - Molecular ecology resources
N2  - Targeted capture coupled with high-throughput sequencing can be used to gain information about nuclear sequence variation at hundreds to thousands of loci. Divergent reference capture makes use of molecular data of one species to enrich target loci in other (related) species. This is particularly valuable for nonmodel organisms, for which often no a priori knowledge exists regarding these loci. Here, we have used targeted capture to obtain data for 809 nuclear coding DNA sequences (CDS) in a nonmodel organism, the Eurasian lynx Lynx lynx, using baits designed with the help of the published genome of a related model organism (the domestic cat Felis catus). Using this approach, we were able to survey intraspecific variation at hundreds of nuclear loci in L. lynx across the species’ European range. A large set of biallelic candidate SNPs was then evaluated using a high-throughput SNP genotyping platform (Fluidigm), which we then reduced to a final 96 SNP-panel based on assay performance and reliability; validation was carried out with 100 additional Eurasian lynx samples not included in the SNP discovery phase. The 96 SNP-panel developed from CDS performed very successfully in the identification of individuals and in population genetic structure inference (including the assignment of individuals to their source population). In keeping with recent studies, our results show that genic SNPs can be valuable for genetic monitoring of wildlife species.
KW  - CDS
KW  - conservation genetics
KW  - Eurasian lynx
KW  - genetic monitoring
KW  - hybridization capture
KW  - single nucleotide polymorphism
Y1  - 2018
U6  - https://doi.org/10.1111/1755-0998.12924
SN  - 1755-098X
SN  - 1755-0998
VL  - 18
IS  - 6
SP  - 1356
EP  - 1373
PB  - Wiley
CY  - Hoboken
ER  -