TY  - JOUR
A1  - Hüttl, Christine
A1  - Hettrich, Cornelia
A1  - Riedel, Melanie
A1  - Henklein, Petra
A1  - Rawel, Harshadrai Manilal
A1  - Bier, Frank Fabian
T1  - Development of Peptidyl Lysine Dendrons: 1,3-Dipolar Cycloaddition for Peptide Coupling and Antibody Recognition
JF  - Chemical biology & drug design
N2  - A straightforward synthesis strategy to multimerize a peptide mimotopes for antibody B13-DE1 recognition is described based on lysine dendrons as multivalent scaffolds. Lysine dendrons that possess N-terminal alkyne residues at the periphery were quantitative functionalized with azido peptides using click chemistry. The solid-phase peptide synthesis (SPPS) allows preparing the peptide dendron in high purity and establishing the possibility of automation. The presented peptide dendron is a promising candidate as multivalent ligand and was used for antibody B13-DE1 recognition. The binding affinity increases with higher dendron generation without loss of specificity. The analysis of biospecific interaction between the synthesized peptide dendron and the antibody was done via surface plasmon resonance (SPR) technique. The presented results show a promising tool for investigations of antigen-antibody reactions.
KW  - click chemistry
KW  - lysine dendron
KW  - peptide mimotopes
KW  - solid-phase peptide synthesis
KW  - surface plasmon resonance
Y1  - 2015
U6  - https://doi.org/10.1111/cbdd.12444
SN  - 1747-0277
SN  - 1747-0285
VL  - 85
IS  - 5
SP  - 565
EP  - 573
PB  - Wiley-Blackwell
CY  - Hoboken
ER  - 
TY  - JOUR
A1  - Anklam, Elke
A1  - Behler, Jörg
A1  - Dingermann, Theodor
A1  - Elsinghorst, Paul
A1  - Fischer, Jochen
A1  - Esselen, Melanie
A1  - Foerster, Christian
A1  - Fröhlich, Daniel
A1  - Goedel, Werner Andreas
A1  - Gregory, Peter
A1  - Grimme, Stefan
A1  - Hackenberger, Christian
A1  - Hansmann, Max
A1  - Heppekausen, Johannes
A1  - Hasenstab-Riedel, Sebastian
A1  - Kirchhoff, Erhard
A1  - Kratz, Karl-Ludwig
A1  - Krausz, Ferenc
A1  - Linker, Torsten
A1  - List, Benjamin
A1  - Ray, Kallol
A1  - Salzer, Reiner
A1  - Schubert, Ulrich
A1  - Schueth, Ferdi
A1  - Schwarz, Helmut
A1  - Schwietzke, Uta
A1  - Strey, Reinhard
A1  - Stumpf, Thorsten
A1  - Vaagt, Franziska
A1  - Volodkin, Dmitry
A1  - Wilke, Guenther
A1  - Zass, Engelbert
A1  - Zemb, Thomas
T1  - Awards
JF  - Nachrichten aus der Chemie : Zeitschrift der Gesellschaft Deutscher Chemiker
Y1  - 2013
U6  - https://doi.org/10.1002/nadc.201390372
SN  - 1439-9598
SN  - 1868-0054
VL  - 61
IS  - 11
SP  - 1145
EP  - 1148
PB  - Ges. Dt. Chemiker
CY  - Frankfurt, Main
ER  -