TY  - GEN
A1  - Krüger, Johanna
A1  - Foerster, Verena Elisabeth
A1  - Trauth, Martin H.
A1  - Hofreiter, Michael
A1  - Tiedemann, Ralph
T1  - Exploring the Past Biosphere of Chew Bahir/Southern Ethiopia: Cross-Species Hybridization Capture of Ancient Sedimentary DNA from a Deep Drill Core
T2  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe
N2  - Eastern Africa has been a prime target for scientific drilling because it is rich in key paleoanthropological sites as well as in paleolakes, containing valuable paleoclimatic information on evolutionary time scales. The Hominin Sites and Paleolakes Drilling Project (HSPDP) explores these paleolakes with the aim of reconstructing environmental conditions around critical episodes of hominin evolution. Identification of biological taxa based on their sedimentary ancient DNA (sedaDNA) traces can contribute to understand past ecological and climatological conditions of the living environment of our ancestors. However, sedaDNA recovery from tropical environments is challenging because high temperatures, UV irradiation, and desiccation result in highly degraded DNA. Consequently, most of the DNA fragments in tropical sediments are too short for PCR amplification. We analyzed sedaDNA in the upper 70 m of the composite sediment core of the HSPDP drill site at Chew Bahir for eukaryotic remnants. We first tested shotgun high throughput sequencing which leads to metagenomes dominated by bacterial DNA of the deep biosphere, while only a small fraction was derived from eukaryotic, and thus probably ancient, DNA. Subsequently, we performed cross-species hybridization capture of sedaDNA to enrich ancient DNA (aDNA) from eukaryotic remnants for paleoenvironmental analysis, using established barcoding genes (cox1 and rbcL for animals and plants, respectively) from 199 species that may have had relatives in the past biosphere at Chew Bahir. Metagenomes yielded after hybridization capture are richer in reads with similarity to cox1 and rbcL in comparison to metagenomes without prior hybridization capture. Taxonomic assignments of the reads from these hybridization capture metagenomes also yielded larger fractions of the eukaryotic domain. For reads assigned to cox1, inferred wet periods were associated with high inferred relative abundances of putative limnic organisms (gastropods, green algae), while inferred dry periods showed increased relative abundances for insects. These findings indicate that cross-species hybridization capture can be an effective approach to enhance the information content of sedaDNA in order to explore biosphere changes associated with past environmental conditions, enabling such analyses even under tropical conditions.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 1244 
KW  - Chew Bahir
KW  - hybridization capture
KW  - ICDP
KW  - paleoclimate
KW  - past biosphere
KW  - sedaDNA
KW  - sediment core
Y1  - 2022
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-550071
SN  - 1866-8372
SP  - 1
EP  - 20
PB  - Universitätsverlag Potsdam
CY  - Potsdam
ER  - 
TY  - GEN
A1  - Cohen, Abby
A1  - Campisano, Christopher
A1  - Arrowsmith, J. Ramon
A1  - Asrat, Asfawossen
A1  - Behrensmeyer, A. K.
A1  - Deino, A.
A1  - Feibel, C.
A1  - Hill, A.
A1  - Johnson, R.
A1  - Kingston, J.
A1  - Lamb, Henry F.
A1  - Lowenstein, T.
A1  - Noren, A.
A1  - Olago, D.
A1  - Owen, Richard Bernhart
A1  - Potts, R.
A1  - Reed, Kate
A1  - Renaut, R.
A1  - Schäbitz, F.
A1  - Tiercelin, J.-J.
A1  - Trauth, Martin  H.
A1  - Wynn, J.
A1  - Ivory, S.
A1  - Brady, K.
A1  - O’Grady, R.
A1  - Rodysill, J.
A1  - Githiri, J.
A1  - Russell, Joellen
A1  - Foerster, Verena
A1  - Dommain, René
A1  - Rucina, J. S.
A1  - Deocampo, D.
A1  - Russell, J.
A1  - Billingsley, A.
A1  - Beck, C.
A1  - Dorenbeck, G.
A1  - Dullo, L.
A1  - Feary, D.
A1  - Garello, D.
A1  - Gromig, R.
A1  - Johnson, T.
A1  - Junginger, Annett
A1  - Karanja, M.
A1  - Kimburi, E.
A1  - Mbuthia, A.
A1  - McCartney, Tannis
A1  - McNulty, E.
A1  - Muiruri, V.
A1  - Nambiro, E.
A1  - Negash, E. W.
A1  - Njagi, D.
A1  - Wilson, J. N.
A1  - Rabideaux, N.
A1  - Raub, Timothy
A1  - Sier, Mark Jan
A1  - Smith, P.
A1  - Urban, J.
A1  - Warren, M.
A1  - Yadeta, M.
A1  - Yost, Chad
A1  - Zinaye, B.
T1  - The Hominin Sites and Paleolakes Drilling Project
BT  - inferring the environmental context of human evolution from eastern African rift lake deposits
T2  - Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe
N2  - The role that climate and environmental history may have played in influencing human evolution has been the focus of considerable interest and controversy among paleoanthropologists for decades. Prior attempts to understand the environmental history side of this equation have centered around the study of outcrop sediments and fossils adjacent to where fossil hominins (ancestors or close relatives of modern humans) are found, or from the study of deep sea drill cores. However, outcrop sediments are often highly weathered and thus are unsuitable for some types of paleoclimatic records, and deep sea core records come from long distances away from the actual fossil and stone tool remains. The Hominin Sites and Paleolakes Drilling Project (HSPDP) was developed to address these issues. The project has focused its efforts on the eastern African Rift Valley, where much of the evidence for early hominins has been recovered. We have collected about 2 km of sediment drill core from six basins in Kenya and Ethiopia, in lake deposits immediately adjacent to important fossil hominin and archaeological sites. Collectively these cores cover in time many of the key transitions and critical intervals in human evolutionary history over the last 4 Ma, such as the earliest stone tools, the origin of our own genus Homo, and the earliest anatomically modern Homo sapiens. Here we document the initial field, physical property, and core description results of the 2012-2014 HSPDP coring campaign.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 611 
KW  - Turkana-Basin
KW  - Adar formation
KW  - climate-change
KW  - olorgesailie formation
KW  - Southern Ethiopia
KW  - global climate
KW  - Kenya Rift
KW  - Pleistocene
KW  - variability
KW  - patterns
Y1  - 2019
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-412498
IS  - 611
ER  -