TY  - JOUR
A1  - Cederkvist, F. Henning
A1  - Zamfir, Alina D.
A1  - Bahrke, Sven
A1  - Eijsink, Vincent G. H.
A1  - Sorlie, Morten
A1  - Peter-Katalinic, Jasna
A1  - Peter, Martin G.
T1  - Identification of a high-affinity-binding oligosaccharide by (+) nanoelectrospray quadrupole time-of-flight tandem mass spectrometry of a noncovalent enzyme-ligand complex
Y1  - 2006
UR  - http://www3.interscience.wiley.com/cgi-bin/jhome/26737/
U6  - https://doi.org/10.1002/anie.200503168
SN  - 1433-7851
ER  - 
TY  - JOUR
A1  - Einarsson, Jon M.
A1  - Bahrke, Sven
A1  - Sigurdsson, Bjarni Thor
A1  - Ng, Chuen-How
A1  - Petersen, Petur Henry
A1  - Sigurjonsson, Olafur E.
A1  - Jonsson, Halldor
A1  - Gislason, Johannes
A1  - Thormodsson, Finnbogi R.
A1  - Peter, Martin G.
T1  - Partially acetylated chitooligosaccharides bind to YKL-40 and stimulate growth of human osteoarthritic chondrocytes
JF  - Biochemical and biophysical research communications
N2  - Recent evidences indicating that cellular kinase signaling cascades are triggered by oligomers of N-acetylglucosamine (ChOS) and that condrocytes of human osteoarthritic cartilage secrete the inflammation associated chitolectin YKL-40, prompted us to study the binding affinity of partially acetylated ChOS to YKL-40 and their effect on primary chondrocytes in culture. Extensive chitinase digestion and filtration of partially deacetylated chitin yielded a mixture of ChOS (Oligomin(TM)) and further ultrafiltration produced T-ChOS(TM), with substantially smaller fraction of the smallest sugars. YKL-40 binding affinity was determined for the different sized homologues, revealing micromolar affinities of the larger homologues to YKL-40. The response of osteoarthritic chondrocytes to Oligomin(TM) and T-ChOS(TM) was determined, revealing 2- to 3-fold increases in cell number. About 500 mu g/ml was needed for Oligomin(TM) and around five times lower concentration for T-ChOS(TM), higher concentrations abolished this effect for both products. Addition of chitotriose inhibited cellular responses mediated by larger oligosaccharides. These results, and the fact that the partially acetylated T-ChOS(TM) homologues should resist hydrolysis, point towards a new therapeutic concept for treating inflammatory joint diseases.
KW  - Cell culture
KW  - Chitolectin
KW  - Chitooligosaccharides
KW  - Chondrocytes
KW  - High affinity binding
KW  - Rheumatoid arthritis
KW  - YKL-40
Y1  - 2013
U6  - https://doi.org/10.1016/j.bbrc.2013.02.122
SN  - 0006-291X
VL  - 434
IS  - 2
SP  - 298
EP  - 304
PB  - Elsevier
CY  - San Diego
ER  -