TY  - JOUR
A1  - Henning, Thorsten
A1  - Kochlik, Bastian Max
A1  - Kusch, Paula
A1  - Strauss, Matthias
A1  - Juric, Viktorija
A1  - Pignitter, Marc
A1  - Marusch, Frank
A1  - Grune, Tilman
A1  - Weber, Daniela
T1  - Pre-Operative assessment of micronutrients, amino acids, phospholipids and oxidative stress in bariatric surgery candidates
JF  - Antioxidants : open access journal
N2  - Obesity has been linked to lower concentrations of fat-soluble micronutrients and higher concentrations of oxidative stress markers as well as an altered metabolism of branched chain amino acids and phospholipids. In the context of morbid obesity, the aim of this study was to investigate whether and to which extent plasma status of micronutrients, amino acids, phospholipids and oxidative stress differs between morbidly obese (n = 23) and non-obese patients (n = 13). In addition to plasma, malondialdehyde, retinol, cholesterol and triglycerides were assessed in visceral and subcutaneous adipose tissue in both groups. Plasma gamma-tocopherol was significantly lower (p < 0.011) in the obese group while other fat-soluble micronutrients showed no statistically significant differences between both groups. Branched-chain amino acids (all p < 0.008) and lysine (p < 0.006) were significantly higher in morbidly obese patients compared to the control group. Malondialdehyde concentrations in both visceral (p < 0.016) and subcutaneous (p < 0.002) adipose tissue were significantly higher in the morbidly obese group while plasma markers of oxidative stress showed no significant differences between both groups. Significantly lower plasma concentrations of phosphatidylcholine, phosphatidylethanolamine, lyso-phosphatidylethanolamine (all p < 0.05) and their corresponding ether-linked analogs were observed, which were all reduced in obese participants compared to the control group. Pre-operative assessment of micronutrients in patients undergoing bariatric surgery is recommended for early identification of patients who might be at higher risk to develop a severe micronutrient deficiency post-surgery. Assessment of plasma BCAAs and phospholipids in obese patients might help to differentiate between metabolic healthy patients and those with metabolic disorders.
KW  - obesity
KW  - micronutrients
KW  - oxidative stress
KW  - amino acids
KW  - phospholipids
KW  - vitamins
KW  - plasma
Y1  - 2022
U6  - https://doi.org/10.3390/antiox11040774
SN  - 2076-3921
VL  - 11
IS  - 4
PB  - MDPI
CY  - Basel
ER  - 
TY  - JOUR
A1  - Nowotny, Kerstin
A1  - Castro, Jose Pedro
A1  - Hugo, Martin
A1  - Braune, Sabine
A1  - Weber, Daniela
A1  - Pignitter, Marc
A1  - Somoza, Veronika
A1  - Bornhorst, Julia
A1  - Schwerdtle, Tanja
A1  - Grune, Tilman
T1  - Oxidants produced by methylglyoxal-modified collagen trigger ER stress and apoptosis in skin fibroblasts
JF  - Free radical biology and medicine : the official journal of the Oxygen Society, a constituent member of the International Society for Free Radical Research
N2  - Methylglyoxal (MG), a highly reactive dicarbonyl, interacts with proteins to form advanced glycation end products (AGEs). AGEs include a variety of compounds which were shown to have damaging potential and to accumulate in the course of different conditions such as diabetes mellitus and aging. After confirming collagen as a main target for MG modifications in vivo within the extracellular matrix, we show here that MG-collagen disrupts fibroblast redox homeostasis and induces endoplasmic reticulum (ER) stress and apoptosis. In particular, MG-collagen-induced apoptosis is associated with the activation of the PERK-eIF2 alpha pathway and caspase-12. MG-collagen contributes to altered redox homeostasis by directly generating hydrogen peroxide and oxygen-derived free radicals. The induction of ER stress in human fibroblasts was confirmed using collagen extracts isolated from old mice in which MG-derived AGEs were enriched. In conclusion, MG-derived AGEs represent one factor contributing to diminished fibroblast function during aging.
KW  - Advanced glycation end products
KW  - Aging
KW  - Apoptosis
KW  - Collagen
KW  - ER stress
KW  - Methylglyoxal
KW  - Redox homeostasis
Y1  - 2018
U6  - https://doi.org/10.1016/j.freeradbiomed.2018.03.022
SN  - 0891-5849
SN  - 1873-4596
VL  - 120
SP  - 102
EP  - 113
PB  - Elsevier
CY  - New York
ER  -