TY - JOUR A1 - Krämer, Nadine A1 - Ravindran, Ethiraj A1 - Zaqout, Sami A1 - Neubert, Gerda A1 - Schindler, Detlev A1 - Ninnemann, Olaf A1 - Gräf, Ralph A1 - Seiler, Andrea E. M. A1 - Kaindl, Angela M. T1 - Loss of CDK5RAP2 affects neural but not non-neural mESC differentiation into cardiomyocytes JF - Cell cycle N2 - Biallelic mutations in the gene encoding centrosomal CDK5RAP2 lead to autosomal recessive primary microcephaly (MCPH), a disorder characterized by pronounced reduction in volume of otherwise architectonical normal brains and intellectual deficit. The current model for the microcephaly phenotype in MCPH invokes a premature shift from symmetric to asymmetric neural progenitor-cell divisions with a subsequent depletion of the progenitor pool. The isolated neural phenotype, despite the ubiquitous expression of CDK5RAP2, and reports of progressive microcephaly in individual MCPH cases prompted us to investigate neural and non-neural differentiation of Cdk5rap2-depleted and control murine embryonic stem cells (mESC). We demonstrate an accumulating proliferation defect of neurally differentiating Cdk5rap2-depleted mESC and cell death of proliferative and early postmitotic cells. A similar effect does not occur in non-neural differentiation into beating cardiomyocytes, which is in line with the lack of non-central nervous system features in MCPH patients. Our data suggest that MCPH is not only caused by premature differentiation of progenitors, but also by reduced propagation and survival of neural progenitors. KW - CDK5RAP2 KW - MCPH KW - mental retardation KW - neural differentiation KW - primary microcephaly KW - stem cell Y1 - 2015 U6 - https://doi.org/10.1080/15384101.2015.1044169 SN - 1538-4101 SN - 1551-4005 VL - 14 IS - 13 SP - 2044 EP - 2057 PB - Taylor & Francis Group CY - Philadelphia ER - TY - GEN A1 - Kaufmann, Yvonne M. A1 - Maiwald, Lisa A1 - Schindler, Svenja A1 - Weck, Florian T1 - Wie wirkt sich mehrmaliges Kompetenz-Feedback auf psychotherapeutische Behandlungen aus? T1 - How does multiple competence feedback affect psychotherapeutic treatment? BT - eine qualitative Analyse BT - a qualitative analysis T2 - Postprints der Universität Potsdam : Humanwissenschaftliche Reihe N2 - Theoretischer Hintergrund: Einflüsse von therapeutenorientiertem Kompetenz-Feedback in der Psychotherapieausbildung wurden bislang wenig untersucht. Fragestellung: Wie gehen Ausbildungstherapeuten mit Feedback um? Welchen Einfluss hat ein regelmäßiges Kompetenz-Feedback auf die Qualität psychotherapeutischer Behandlungen (insbesondere Therapiesitzungen, therapeutische Beziehung, Person des Therapeuten, Supervision)? Methode: Elf Therapeuten wurden mithilfe eines halbstrukturierten Interviewleitfadens befragt. Die Auswertung erfolgte mittels qualitativer Inhaltsanalyse nach Mayring (2015). Ergebnisse: Das auf Basis der Interviews erstellte Kategoriensystem umfasste die Kategorien „Erwartungen an das Feedback“, „Wahrnehmung des Feedbacks“, „Verarbeitung von und Umgang mit Feedback“, „Folgen, Auswirkungen und Veränderungen durch Feedback“ sowie „Verbesserungswünsche“. Schlussfolgerungen: Therapeuten streben eine Umsetzung des Feedbacks an, welches sich auf die Behandlung, die Supervision, die eigene Person und die therapeutische Beziehung auswirkt. N2 - Background: To date, the influence of therapist-oriented feedback in psychotherapy training has rarely been investigated. Objective: How do therapists in training deal with competence feedback? What influence does a regular competence feedback have on treatment quality (especially therapy sessions, therapeutic alliance, the therapist, supervision)? Method: We interviewed 11 therapists using a semistructured interview guide. The text material was analyzed with qualitative content analysis (Mayring, 2015). Results: The interview-based system of categories consists of: expectations toward feedback; perception of feedback; processing of feedback; consequences, effects, and changes due to feedback; and suggestions for improvement. Conclusion: Therapists strive to implement feedback. Competence feedback has an impact on treatment, supervision, the therapist, and the therapeutic alliance. T3 - Zweitveröffentlichungen der Universität Potsdam : Humanwissenschaftliche Reihe - 614 KW - Feedback KW - Psychotherapeutische Ausbildung KW - Psychotherapeutische Kompetenzen KW - Qualitative Inhaltsanalyse KW - Supervision KW - feedback KW - psychotherapy training KW - psychotherapeutic competencies KW - qualitative content analysis KW - clinical supervision Y1 - 2020 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-434066 SN - 1866-8364 IS - 614 SP - 96 EP - 106 ER - TY - JOUR A1 - Salmen, T. A1 - Ermakova, Tatiana A1 - Schindler, A. A1 - Ko, S-R. A1 - Göktas, O. A1 - Gross, M. A1 - Nawka, T. A1 - Caffier, P. P. T1 - Efficacy of microsurgery in Reinke's oedema evaluated by traditional voice assessment integrated with the Vocal Extent Measure (VEM) JF - Acta Otorhinolaryngol Italica N2 - There are few data analysing to what specific extent phonomicrosurgery improves vocal function in patients suffering from Reinke's oedema (RE). The recently introduced parameter vocal extent measure (VEM) seems to be suitable to objectively quantify vocal performance. The purpose of this clinical prospective study was to investigate the outcomes of phonomicrosurgery in 60 RE patients (6 male, 54 female; 56 ± 8 years ([mean ± SD]) by analysing its effect on subjective and objective vocal parameters with particular regard to VEM. Treatment efficacy was evaluated at three months after surgery by comparing pre- and postoperative videolaryngostroboscopy (VLS), auditory-perceptual assessment (RBH-status), voice range profile (VRP), acoustic-aerodynamic analysis and patient's self-assessment using the voice handicap index (VHI-9i). Phonomicrosurgically, all RE were carefully ablated. VLS revealed removal or substantial reduction of oedema with restored periodic vocal fold vibration. All subjective and most objective acoustic and aerodynamic parameters significantly improved. The VEM increased on average from 64 ± 37 to 88 ± 25 (p #x003C; 0.001) and the dysphonia severity index (DSI) from 0.5 ± 3.4 to 2.9 ± 1.9. Both parameters correlated significantly with each other (rs = 0.70). RBH-status revealed less roughness, breathiness and overall grade of hoarseness (2.0 ± 0.7 vs 1.3 ± 0.7). The VHI-9i-score decreased from 18 ± 8 to 12 ± 9 points. The average total vocal range enlarged by 4 ± 7 semitones, and the mean speaking pitch rose by 2 ± 4 semitones. These results confirm that: (1) the use of VEM in RE patients objectifies and quantifies their vocal capacity as documented in the VRP, and (2) phonomicrosurgery is an effective, objectively and subjectively satisfactory therapy to improve voice in RE patients. KW - Voice function diagnostics KW - Voice range profile quantification KW - Vocal Extent Measure (VEM) KW - Reinke’s oedema KW - Phonomicrosurgery Y1 - 2018 U6 - https://doi.org/10.14639/0392-100X-1544 SN - 0392-100X SN - 1827-675X VL - 38 IS - 3 SP - 194 EP - 203 PB - Pacini editore CY - Pisa ER - TY - JOUR A1 - Rothe, Heinz-Jürgen A1 - Schindler, M. T1 - Expertiese und Wissen Y1 - 1996 ER - TY - JOUR A1 - Hagen, Sven A1 - Baumann, Tobias A1 - Wagner, Hanna J. A1 - Morath, Volker A1 - Kaufmann, Beate A1 - Fischer, Adrian A1 - Bergmann, Stefan A1 - Schindler, Patrick A1 - Arndt, Katja Maren A1 - Mueller, Kristian M. T1 - Modular adeno-associated virus (rAAV) vectors used for cellular virus-directed enzyme prodrug therapy JF - Scientific reports N2 - The pre-clinical and clinical development of viral vehicles for gene transfer increased in recent years, and a recombinant adeno-associated virus (rAAV) drug took center stage upon approval in the European Union. However, lack of standardization, inefficient purification methods and complicated retargeting limit general usability. We address these obstacles by fusing rAAV-2 capsids with two modular targeting molecules (DARPin or Affibody) specific for a cancer cell-surface marker (EGFR) while simultaneously including an affinity tag (His-tag) in a surface-exposed loop. Equipping these particles with genes coding for prodrug converting enzymes (thymidine kinase or cytosine deaminase) we demonstrate tumor marker specific transduction and prodrug-dependent apoptosis of cancer cells. Coding terminal and loop modifications in one gene enabled specific and scalable purification. Our genetic parts for viral production adhere to a standardized cloning strategy facilitating rapid prototyping of virus directed enzyme prodrug therapy (VDEPT). Y1 - 2014 U6 - https://doi.org/10.1038/srep03759 SN - 2045-2322 VL - 4 PB - Nature Publ. Group CY - London ER - TY - GEN A1 - Schindler, Sebastian A1 - Wolff, Wanja A1 - Kissler, Johanna M. A1 - Brand, Ralf T1 - Cerebral correlates of faking BT - evidence from a brief implicit association test on doping attitudes T2 - Postprints der Universität Potsdam : Humanwissenschaftliche Reihe N2 - Direct assessment of attitudes toward socially sensitive topics can be affected by deception attempts. Reaction-time based indirect measures, such as the Implicit Association Test (IAT), are less susceptible to such biases. Neuroscientific evidence shows that deception can evoke characteristic ERP differences. However, the cerebral processes involved in faking an IAT are still unknown. We randomly assigned 20 university students (15 females, 24.65 +/- 3.50 years of age) to a counterbalanced repeated-measurements design, requesting them to complete a Brief-IAT (BIAT) on attitudes toward doping without deception instruction, and with the instruction to fake positive and negative doping attitudes. Cerebral activity during BIAT completion was assessed using high-density EEG. Event-related potentials during faking revealed enhanced frontal and reduced occipital negativity, starting around 150 ms after stimulus presentation. Further, a decrease in the P300 and LPP components was observed. Source analyses showed enhanced activity in the right inferior frontal gyrus between 150 and 200 ms during faking, thought to reflect the suppression of automatic responses. Further, more activity was found for faking in the bilateral middle occipital gyri and the bilateral temporoparietal junction. Results indicate that faking reaction-time based tests alter brain processes from early stages of processing and reveal the cortical sources of the effects. Analyzing the EEG helps to uncover response patterns in indirect attitude tests and broadens our understanding of the neural processes involved in such faking. This knowledge might be useful for uncovering faking in socially sensitive contexts, where attitudes are likely to be concealed. T3 - Zweitveröffentlichungen der Universität Potsdam : Humanwissenschaftliche Reihe - 419 KW - EEG/ERP KW - implicit association test (IAT) KW - faking KW - deception KW - indirect tests KW - anti-doping KW - right inferior frontal gyrus Y1 - 2018 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-406251 IS - 419 ER - TY - JOUR A1 - Schindler, Sebastian A1 - Wolff, Wanja A1 - Kissler, Johanna M. A1 - Brand, Ralf T1 - Cerebral correlates of faking BT - evidence from a brief implicit association test on doping attitudes JF - Frontiers in Behavioral Neuroscience N2 - Direct assessment of attitudes toward socially sensitive topics can be affected by deception attempts. Reaction-time based indirect measures, such as the Implicit Association Test (IAT), are less susceptible to such biases. Neuroscientific evidence shows that deception can evoke characteristic ERP differences. However, the cerebral processes involved in faking an IAT are still unknown. We randomly assigned 20 university students (15 females, 24.65 +/- 3.50 years of age) to a counterbalanced repeated-measurements design, requesting them to complete a Brief-IAT (BIAT) on attitudes toward doping without deception instruction, and with the instruction to fake positive and negative doping attitudes. Cerebral activity during BIAT completion was assessed using high-density EEG. Event-related potentials during faking revealed enhanced frontal and reduced occipital negativity, starting around 150 ms after stimulus presentation. Further, a decrease in the P300 and LPP components was observed. Source analyses showed enhanced activity in the right inferior frontal gyrus between 150 and 200 ms during faking, thought to reflect the suppression of automatic responses. Further, more activity was found for faking in the bilateral middle occipital gyri and the bilateral temporoparietal junction. Results indicate that faking reaction-time based tests alter brain processes from early stages of processing and reveal the cortical sources of the effects. Analyzing the EEG helps to uncover response patterns in indirect attitude tests and broadens our understanding of the neural processes involved in such faking. This knowledge might be useful for uncovering faking in socially sensitive contexts, where attitudes are likely to be concealed. KW - EEG/ERP KW - implicit association test (IAT) KW - faking KW - deception KW - indirect tests KW - anti-doping KW - right inferior frontal gyrus Y1 - 2015 U6 - https://doi.org/10.3389/fnbeh.2015.00139 SN - 1662-5153 VL - 9 SP - 1 EP - 13 PB - Frontiers Research Foundation CY - Lausanne ER -