TY - GEN A1 - Szarzynska, Bogna A1 - Sobkowiak, Lukasz A1 - Pant, Bikram Datt A1 - Balazadeh, Salma A1 - Scheible, Wolf-Rüdiger A1 - Müller-Röber, Bernd A1 - Jarmolowski, Artur A1 - Szweykowska-Kulinska, Zofia T1 - Gene structures and processing of Arabidopsis thaliana HYL1-dependent pri-miRNAs N2 - Arabidopsis thaliana HYL1 is a nuclear doublestranded RNA-binding protein involved in the maturation of pri-miRNAs. A quantitative real-time PCR platform for parallel quantification of 176 primiRNAs was used to reveal strong accumulation of 57 miRNA precursors in the hyl1 mutant that completely lacks HYL1 protein. This approach enabled us for the first time to pinpoint particular members of MIRNA family genes that require HYL1 activity for efficient maturation of their precursors. Moreover, the accumulation of miRNA precursors in the hyl1 mutant gave us the opportunity to carry out 3’ and 5’ RACE experiments which revealed that some of these precursors are of unexpected length. The alignment of HYL1- dependent miRNA precursors to A. thaliana genomic sequences indicated the presence of introns in 12 out of 20 genes studied. Some of the characterized intron-containing pri-miRNAs undergo alternative splicing such as exon skipping or usage of alternative 5’ splice sites suggesting that this process plays a role in the regulation of miRNA biogenesis. In the hyl1 mutant intron-containing pri-miRNAs accumulate alongside spliced primiRNAs suggesting the recruitment of HYL1 into the miRNA precursor maturation pathway before their splicing occurs. T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - paper 138 KW - Binding-protein hyl1 KW - Abscisic-acid KW - Flowering time KW - Micro-RNA KW - Serrate Y1 - 2009 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-45085 ER - TY - JOUR A1 - Rössler, Patrick A1 - Bomhoff, Jana A1 - Haschke, Josef Ferdinand A1 - Kersten, Jan A1 - Müller, Rüdiger T1 - Selection and impact of press photography BT - an empirical study on the basis of photo news factors JF - Communications : the European journal of communication research N2 - The selection of 'good' pictures has increasingly become a crucial factor when transmitting news to the recipients. Every day thousands of events are happening and millions of pictures are taken. By choosing photographs for newspapers and magazines, photographic editorial departments want to attract the recipients' attention, evoke emotions and get them to read their stories. But what exactly is a good picture that meets these expectations? Which criteria are decisive for selecting pictures and what effects of this selection can be measured on the recipients' side? This article presents the results of a research project carried out at the University of Erfurt in 2008 and conducted in collaboration with the German weekly magazine stern. It deals with the selection and impact of press photography by introducing the concept 'photo news factors'. Applying the traditional news value theory to pictures, photo news factors are defined as selection criteria that, on the part of the communicator, decide whether the press photos are worth publishing. Furthermore, they are assumed to exert an influence on the intensity of attention that a picture arouses. KW - press photography KW - news value theory KW - photo news factors selectivity KW - media effects Y1 - 2011 U6 - https://doi.org/10.1515/COMM.2011.021 SN - 0341-2059 VL - 36 IS - 4 SP - 415 EP - 439 PB - De Gruyter Mouton CY - Berlin ER - TY - GEN A1 - Rössler, Patrick A1 - Bomhoff, Jana A1 - Haschke, Josef Ferdinand A1 - Kersten, Jan A1 - Müller, Rüdiger T1 - Selection and impact of press photography BT - an empirical study on the basis of photo news factors T2 - Postprints der Universität Potsdam : Philosophische Reihe N2 - The selection of ‘good’ pictures has increasingly become a crucial factor when transmitting news to the recipients. Every day thousands of events are happening and millions of pictures are taken. By choosing photographs for newspapers and magazines, photographic editorial departments want to attract the recipients’ attention, evoke emotions and get them to read their stories. But what exactly is a good picture that meets these expectations? Which criteria are decisive for selecting pictures and what effects of this selection can be measured on the recipients’ side? This article presents the results of a research project carried out at the University of Erfurt in 2008 and conducted in collaboration with the German weekly magazine stern. It deals with the selection and impact of press photography by introducing the concept ‘photo news factors’. Applying the traditional news value theory to pictures, photo news factors are defined as selection criteria that, on the part of the communicator, decide whether the press photos are worth publishing. Furthermore, they are assumed to exert an influence on the intensity of attention that a picture arouses. T3 - Zweitveröffentlichungen der Universität Potsdam : Philosophische Reihe - 103 KW - press photography KW - news value theory KW - photo news factors KW - selectivity KW - media effects Y1 - 2016 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-93694 SN - 1866-8380 IS - 103 ER - TY - BOOK A1 - Schwarzer, Ingo A1 - Weiß-Saoumi, Said A1 - Kittel, Roland A1 - Friedrich, Tobias A1 - Kaynak, Koraltan A1 - Durak, Cemil A1 - Isbarn, Andreas A1 - Diestel, Jörg A1 - Knittel, Jens A1 - Franz, Marquart A1 - Morra, Carlos A1 - Stahnke, Susanne A1 - Braband, Jens A1 - Dittmann, Johannes A1 - Griebel, Stephan A1 - Krampf, Andreas A1 - Link, Martin A1 - Müller, Matthias A1 - Radestock, Jens A1 - Strub, Leo A1 - Bleeke, Kai A1 - Jehl, Leander A1 - Kapitza, Rüdiger A1 - Messadi, Ines A1 - Schmidt, Stefan A1 - Schwarz-Rüsch, Signe A1 - Pirl, Lukas A1 - Schmid, Robert A1 - Friedenberger, Dirk A1 - Beilharz, Jossekin Jakob A1 - Boockmeyer, Arne A1 - Polze, Andreas A1 - Röhrig, Ralf A1 - Schäbe, Hendrik A1 - Thiermann, Ricky T1 - RailChain BT - Abschlussbericht N2 - The RailChain project designed, implemented, and experimentally evaluated a juridical recorder that is based on a distributed consensus protocol. That juridical blockchain recorder has been realized as distributed ledger on board the advanced TrainLab (ICE-TD 605 017) of Deutsche Bahn. For the project, a consortium consisting of DB Systel, Siemens, Siemens Mobility, the Hasso Plattner Institute for Digital Engineering, Technische Universität Braunschweig, TÜV Rheinland InterTraffic, and Spherity has been formed. These partners not only concentrated competencies in railway operation, computer science, regulation, and approval, but also combined experiences from industry, research from academia, and enthusiasm from startups. Distributed ledger technologies (DLTs) define distributed databases and express a digital protocol for transactions between business partners without the need for a trusted intermediary. The implementation of a blockchain with real-time requirements for the local network of a railway system (e.g., interlocking or train) allows to log data in the distributed system verifiably in real-time. For this, railway-specific assumptions can be leveraged to make modifications to standard blockchains protocols. EULYNX and OCORA (Open CCS On-board Reference Architecture) are parts of a future European reference architecture for control command and signalling (CCS, Reference CCS Architecture – RCA). Both architectural concepts outline heterogeneous IT systems with components from multiple manufacturers. Such systems introduce novel challenges for the approved and safety-relevant CCS of railways which were considered neither for road-side nor for on-board systems so far. Logging implementations, such as the common juridical recorder on vehicles, can no longer be realized as a central component of a single manufacturer. All centralized approaches are in question. The research project RailChain is funded by the mFUND program and gives practical evidence that distributed consensus protocols are a proper means to immutably (for legal purposes) store state information of many system components from multiple manufacturers. The results of RailChain have been published, prototypically implemented, and experimentally evaluated in large-scale field tests on the advanced TrainLab. At the same time, the project showed how RailChain can be integrated into the road-side and on-board architecture given by OCORA and EULYNX. Logged data can now be analysed sooner and also their trustworthiness is being increased. This enables, e.g., auditable predictive maintenance, because it is ensured that data is authentic and unmodified at any point in time. N2 - Das Projekt RailChain hat einen verteilten Juridical Recorder entworfen, implementiert und experimentell evaluiert, der auf einem echtzeitfähigen verteilten Konsensprotokoll basiert. Dieser Juridical Blockchain Recorder wurde als distributed ledger an Bord des advanced TrainLabs der Deutschen Bahn (ICE-TD 605 017) umgesetzt. Für das Projekt hat sich ein Konsortium aus DB Systel, Siemens, Siemens Mobility, dem Hasso-Plattner-Institut für Digital Engineering, der Technischen Universität Braunschweig, sowie TÜV Rheinland InterTraffic und Spherity formiert und dabei Kompetenzen aus den Bereichen Bahnbetrieb, Informatik und Zulassungswesen gebündelt. Die Partner kombinieren Erfahrungen aus der Industrie und die akademische Forschung mit der Aufbruchstimmung aus dem Start-Up-Umfeld. Distributed-Ledger-Technologien (DLTs) definieren verteilte Datenbanken und stellen ein digitales Protokoll für Transaktionen zwischen Geschäftspartnern dar, ohne dass ein Mittelsmann beteiligt sein müsste. Die Implementierung einer Blockchain mit Echtzeitanforderungen für das lokale Netzwerk einer Eisenbahnanlage (z. B. Stellwerk oder Zug) erlaubt es, die im verteilten System entstehenden Daten nachweislich in Echtzeit zu protokollieren. Dabei können eisenbahnspezifische Randbedingungen ausgenutzt werden, um Standard-Blockchain-Protokolle anzupassen. EULYNX und OCORA (Open CCS On-board Reference Architecture) sind Bestandteile einer zukünftigen europäischen Referenzarchitektur für das Leit- und Sicherungssystem (Reference CCS Architecture – RCA, Control Command and Signalling – CCS). Beide Architekturkonzepte skizzieren herstellerübergreifende, komponentenbasierende heterogene IT-Systeme. Solche Systeme bergen neue Herausforderungen, die bislang im Kontext der zugelassenen, sicherheitsrelevanten Leit- und Sicherungstechnik der Bahn weder strecken- noch fahrzeugseitig adressiert werden mussten. Logbuch-Implementierungen, wie der gängige Juridical Recorder auf Fahrzeugen, können nun nicht mehr als zentrale Systemkomponente eines einzelnen Herstellers umgesetzt werden. Alle zentralisierten Lösungsansätze sind in Frage gestellt. Das mFUND-geförderte Forschungsprojekt erbringt den praktischen Nachweis, dass Zustandsinformationen über eine Vielzahl von Systemkomponenten herstellerübergreifend und gerichtsfest mittels verteilten Konsensprotokollen gespeichert werden können. Ergebnisse von RailChain wurden publiziert, prototypisch implementiert und in großen Feldtests auf dem advanced TrainLab experimentell evaluiert. Gleichzeitig wurde aufgezeigt, wie sich RailChain in den mit OCORA und EULYNX vorgegebenen fahrzeug- und streckenseitigen Architekturentwurf integrieren lässt. Daten können dadurch zeitnaher ausgewertet werden und gleichzeitig wird ihre Vertrauenswürdigkeit erhöht. Dies ermöglicht u. a. nachvollziehbare zustandsorientierte Wartung, denn es kann jederzeit sichergestellt werden, dass die Daten authentisch sind und auch nicht verändert wurden. T3 - Technische Berichte des Hasso-Plattner-Instituts für Digital Engineering an der Universität Potsdam - 152 KW - Distributed-Ledger-Technologie (DLT) KW - juridical recording KW - Konsensprotokolle KW - consensus protocols KW - Digitalisierung KW - digitalization KW - Bahnwesen KW - railways KW - Blockchain KW - asset management KW - selbstbestimmte Identitäten KW - self-sovereign identity KW - dezentrale Identitäten KW - decentral identities KW - überprüfbare Nachweise KW - verifiable credentials KW - Echtzeit KW - real-time KW - Standardisierung KW - standardization KW - Verlässlichkeit KW - dependability KW - Fehlertoleranz KW - fault tolerance Y1 - 2023 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-577409 SN - 978-3-86956-550-7 SN - 1613-5652 SN - 2191-1665 IS - 152 PB - Universitätsverlag Potsdam CY - Potsdam ER -