TY - JOUR A1 - Li, Mingjun A1 - Gao, Lingyan A1 - Schlaich, Christoph A1 - Zhang, Jianguang A1 - Donskyi, Ievgen S. A1 - Yu, Guozhi A1 - Li, Wenzhong A1 - Tu, Zhaoxu A1 - Rolff, Jens A1 - Schwerdtle, Tanja A1 - Haag, Rainer A1 - Ma, Nan T1 - Construction of Functional Coatings with Durable and Broad-Spectrum Antibacterial Potential Based on Mussel-Inspired Dendritic Polyglycerol and in Situ-Formed Copper Nanoparticles JF - ACS applied materials & interfaces N2 - A novel surface coating with durable broad-spectrum antibacterial ability was prepared based on mussel inspired dendritic polyglycerol (MI-dPG) embedded with copper nanoparticles (Cu NPs). The functional surface coating is fabricated via a facile dip-coating process followed by in situ reduction of copper ions with a MI-dPG coating to introduce Cu NPs into the coating matrix. This coating has been demonstrated to possess efficient long-term antibacterial properties against Escherichia coli (E. coli), Staphylococcus aureus (S. aureus), and kanamycin-resistant E. coli through an "attract-kill-release" strategy. The synergistic antibacterial activity of the coating was shown by the combination of two functions of the contact killing, reactive oxygen species production and Cu ions released from the coating. Furthermore, this coating inhibited biofilm formation and showed good compatibility to eukaryotic cells. Thus, this newly developed Cu NP-incorporated MI-dPG surface coating may find potential application in the design of antimicrobial coating, such as implantable devices. KW - Cu NP-incorporated MI-dPG coating KW - universal coating KW - in situ chemical reduction KW - antibacterial effect KW - drug-resistant bacteria Y1 - 2017 U6 - https://doi.org/10.1021/acsami.7b10541 SN - 1944-8244 VL - 9 SP - 35411 EP - 35418 PB - American Chemical Society CY - Washington ER - TY - JOUR A1 - Li, Mingjun A1 - Schlaich, Christoph A1 - Kulka, Michael Willem A1 - Donskyi, Ievgen S. A1 - Schwerdtle, Tanja A1 - Unger, Wolfgang E. S. A1 - Haag, Rainer T1 - Mussel-inspired coatings with tunable wettability, for enhanced antibacterial efficiency and reduced bacterial adhesion JF - Journal of materials chemistry : B, Materials for biology and medicine N2 - Over the last few decades, there has been a tremendous increase in research on antibacterial surface coatings as an alternative strategy against bacterial infections. Although there are several examples of effective strategies to prevent bacterial adhesion, the effect of the wetting properties on the coating was rarely considered as a crucial factor. Here we report an in-depth study on the effect of extreme wettability on the antibacterial efficiency of a silver nanoparticles ( AgNPs)-based coating. By controlling surface polymerization of mussel-inspired dendritic polyglycerol ( MI-dPG) and post-functionalization, surfaces with wetting properties ranging from superhydrophilic to superhydrophobic were fabricated. Subsequently, AgNPs were embedded into the coatings by applying in situ reduction using the free catechols-moieties present in the MI-dPG coating. The resulting polymer coatings exhibited excellent antibacterial ability against planktonic Escherichia coli ( E. coli) DH5a and Staphylococcus aureus ( S. aureus) SH1000. The antibacterial efficiency of the coatings was analyzed by using inductively coupled plasma mass spectrometry ( ICP-MS) and bacterial viability tests. Furthermore, the antifouling properties of the coatings in relation to the antibacterial properties were evaluated. Y1 - 2019 U6 - https://doi.org/10.1039/c9tb00534j SN - 2050-750X SN - 2050-7518 VL - 7 IS - 21 SP - 3438 EP - 3445 PB - Royal Society of Chemistry CY - Cambridge ER - TY - JOUR A1 - Li, Mingjun A1 - Schlaich, Christoph A1 - Zhang, Jianguang A1 - Donskyi, Ievgen A1 - Schwibbert, Karin A1 - Schreiber, Frank A1 - Xia, Yi A1 - Radnik, Jörg A1 - Schwerdtle, Tanja A1 - Haag, Rainer T1 - Mussel-inspired multifunctional coating for bacterial infection prevention and osteogenic induction JF - Journal of materials science & technology : JMST ; an international journal / spons. by the Chinese Society for Metals (CSM), the Chinese Materials Research Society (CMRS), Institute of Metal Research, Chinese Academy of Sciences N2 - Bacterial infection and osteogenic integration are the two main problems that cause severe complications after surgeries. In this study, the antibacterial and osteogenic properties were simultaneously introduced in biomaterials, where copper nanoparticles (CuNPs) were generated by in situ reductions of Cu ions into a mussel-inspired hyperbranched polyglycerol (MI-hPG) coating via a simple dip-coating method. This hyperbranched polyglycerol with 10 % catechol groups' modification presents excellent antifouling property, which could effectively reduce bacteria adhesion on the surface. In this work, polycaprolactone (PCL) electrospun fiber membrane was selected as the substrate, which is commonly used in biomedical implants in bone regeneration and cardiovascular stents because of its good biocompatibility and easy post-modification. The as-fabricated CuNPs-incorporated PCL membrane [PCL-(MI-hPG)-CuNPs] was confirmed with effective antibacterial performance via in vitro antibacterial tests against Staphylococcus aureus (S. aureus), Escherichia coli (E. coli), and multi-resistant E. coli. In addition, the in vitro results demonstrated that osteogenic property of PCL-(MI-hPG)-CuNPs was realized by upregulating the osteoblast-related gene expressions and protein activity. This study shows that antibacterial and osteogenic properties can be balanced in a surface coating by introducing CuNPs. KW - Mussel-inspired coating KW - CuNPs KW - Multi-resistant bacteria KW - Antibacterial KW - Antifouling KW - Osteogenesis Y1 - 2021 U6 - https://doi.org/10.1016/j.jmst.2020.08.011 SN - 1005-0302 SN - 1941-1162 VL - 68 SP - 160 EP - 171 PB - Elsevier CY - Amsterdam [u.a.] ER -