TY - JOUR A1 - Kunze, Sebastian T1 - Konzessionsverträge – Handlungsoptionen für Kommunen JF - KWI-Schriften N2 - Inhalt: I. Einleitung – Befundnahme II. Vorüberlegungen der Rekommunalisierungsentscheidung in der Energiewirtschaft 1. Zieldreieck der Energiepolitik 2. Ziele kommunaler Energiepolitik 3. Zuordnung der Ziele unter besonderer Berücksichtigung des Energiemarktes 4. Mythos Eigenkapitalrendite im Netzbetrieb und Strompreisregulierung III. Konkrete Möglichkeiten der Konzessionsvergabe 1. Wegenutzungsvertrag mit dem bisherigen Netzbetreiber 2. Wegenutzungsvertrag mit einem neuen Netzbetreiber 3. Beteiligungsmodelle a) Klassisches Partnermodell b) Dienstleistermodell c) Pachtmodell IV. Risiken der Rekommunalisierung 1. Kaufpreis, Entflechtungs- und Einbindungskosten 2. Gewerbesteuer 3. Personal 4. Keine Vergleichbarkeit mit früheren Stadtwerkegründungen V. Auswirkungen regionaler Rekommunalisierungen VI. Fazit Y1 - 2012 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-62280 SN - 1867-951X SN - 1867-9528 IS - 6 SP - 99 EP - 109 PB - Universitätsverlag Potsdam CY - Potsdam ER - TY - JOUR A1 - Keller, Sebastian A1 - Kunze, Cindy A1 - Bommer, Martin A1 - Paetz, Christian A1 - Menezes, Riya C. A1 - Svatos, Ales A1 - Dobbek, Holger A1 - Schubert, Torsten T1 - Selective Utilization of Benzimidazolyl-Norcobamides as Cofactors by the Tetrachloroethene Reductive Dehalogenase of Sulfurospirillum multivorans JF - Journal of bacteriology N2 - The organohalide-respiring bacterium Sulfurospirillum multivorans produces a unique cobamide, namely, norpseudo-B-12, which serves as cofactor of the tetrachloroethene (PCE) reductive dehalogenase (PceA). As previously reported, a replacement of the adeninyl moiety, the lower base of the cofactor, by exogenously applied 5,6-dimethylbenzimidazole led to inactive PceA. To explore the general effect of benzimidazoles on the PCE metabolism, the susceptibility of the organism for guided biosynthesis of various singly substituted benzimidazolyl-norcobamides was investigated, and their use as cofactor by PceA was analyzed. Exogenously applied 5-methylbenzimidazole (5-MeBza), 5-hydroxybenzimidazole (5-OHBza), and 5-methoxybenzimidazole (5-OMeBza) were found to be efficiently incorporated as lower bases into norcobamides (NCbas). Structural analysis of the NCbas by nuclear magnetic resonance spectroscopy uncovered a regioselectivity in the utilization of these precursors for NCba biosynthesis. When 5-MeBza was added, a mixture of 5-MeBza-norcobamide and 6-MeBza-norcobamide was formed, and the PceA enzyme activity was affected. In the presence of 5-OHBza, almost exclusively 6-OHBza-norcobamide was produced, while in the presence of 5-OMeBza, predominantly 5-OMeBza-norcobamide was detected. Both NCbas were incorporated into PceA, and no negative effect on the PceA activity was observed. In crystal structures of PceA, both NCbas were bound in the base-off mode with the 6-OHBza and 5-OMeBza lower bases accommodated by the same solvent-exposed hydrophilic pocket that harbors the adenine as the lower base of authentic norpseudo-B-12. In this study, a selective production of different norcobamide isomers containing singly substituted benzimidazoles as lower bases is shown, and unique structural insights into their utilization as co-factors by a cobamide-containing enzyme are provided. IMPORTANCE Guided biosynthesis of norcobamides containing singly substituted benzimidazoles as lower bases by the organohalide-respiring epsilonproteobacterium Sulfurospirillum multivorans is reported. An unprecedented specificity in the formation of norcobamide isomers containing hydroxylated or methoxylated benzimidazoles was observed that implicated a strict regioselectivity of the norcobamide biosynthesis in the organism. In contrast to 5,6-dimethylbenzimidazolyl-norcobamide, the incorporation of singly substituted benzimidazolyl-norcobamides as a cofactor into the tetrachloroethene reductive dehalogenase was not impaired. The enzyme was found to be functional with different isomers and not limited to the use of adeninyl-norcobamide. Structural analysis of the enzyme equipped with either adeninyl-or benzimidazolyl-norcobamide cofactors visualized for the first time structurally different cobamides bound in base-off conformation to the cofactor-binding site of a cobamide-containing enzyme. KW - benzimidazoles KW - corrinoid-containing enzymes KW - reductive dehalogenase KW - vitamin B-12 Y1 - 2018 U6 - https://doi.org/10.1128/JB.00584-17 SN - 0021-9193 SN - 1098-5530 VL - 200 IS - 8 PB - American Society for Microbiology CY - Washington ER - TY - JOUR A1 - Köslin-Findeklee, Fabian A1 - Rizi, Vajiheh Safavi A1 - Becker, Martin A. A1 - Parra-Londono, Sebastian A1 - Arif, Muhammad A1 - Balazadeh, Salma A1 - Müller-Röber, Bernd A1 - Kunze, Reinhard A1 - Horst, Walter J. T1 - Transcriptomic analysis of nitrogen starvation- and cultivar-specific leaf senescence in winter oilseed rape (Brassica napus L.) JF - Plant science : an international journal of experimental plant biology N2 - High nitrogen (N) efficiency, characterized by high grain yield under N limitation, is an important agricultural trait in Brassica napus L. cultivars related to delayed senescence of older leaves during reproductive growth (a syndrome called stay-green). The aim of this study was thus to identify genes whose expression is specifically altered during N starvation-induced leaf senescence and that can be used as markers to distinguish cultivars at early stages of senescence prior to chlorophyll loss. To this end, the transcriptomes of leaves of two B. napus cultivars differing in stay-green characteristics and N efficiency were analyzed 4 days after the induction of senescence by either N starvation, leaf shading or detaching. In addition to N metabolism genes, N starvation mostly (and specifically) repressed genes related to photosynthesis, photorespiration and cell-wall structure, while genes related to mitochondrial electron transport and flavonoid biosynthesis were predominately up-regulated. A kinetic study over a period of 12 days with four B. napus cultivars differing in their stay-green characteristics confirmed the cultivar-specific regulation of six genes in agreement with their senescence behavior: the senescence regulator ANAC029, the anthocyanin synthesis-related genes ANS and DFR-like1, the ammonium transporter AMT1:4, the ureide transporter UPSS, and SPS1 involved in sucrose biosynthesis. The identified genes represent markers for the detection of cultivar-specific differences in N starvation-induced leaf senescence and can thus be employed as valuable tools in B. napus breeding. (C) 2015 Elsevier Ireland Ltd. All rights reserved. KW - Brassica napus KW - Genotypic differences KW - Leaf senescence KW - Molecular marker KW - N efficiency KW - Stay-green Y1 - 2015 U6 - https://doi.org/10.1016/j.plantsci.2014.11.018 SN - 0168-9452 VL - 233 SP - 174 EP - 185 PB - Elsevier CY - Clare ER - TY - BOOK A1 - Bauer, Hartmut A1 - Mühl-Jäckel, Margarete A1 - Becker, Ulrich A1 - Brüning, Christoph A1 - Schäfer, Roland A1 - Röber, Manfred A1 - Kunze, Sebastian ED - Bauer, Hartmut ED - Büchner, Christiane ED - Hajasch, Lydia T1 - Rekommunalisierung öffentlicher Daseinsvorsorge N2 - Mehr Privat statt Staat! Diese Kampfformel galt vielen noch vor kurzem als Schlüssel zur erfolgreichen Entlastung der angespannten kommunalen Haushalte. Immer mehr Kommunen beschritten den vermeintlichen Königsweg. So vielfältig wie die Gegenstände sind auch die in der Verwaltungspraxis zu beobachtenden Erscheinungsformen der Privatisierung: Vermögensprivatisierung, Organisationsprivatisierung, Aufgabenprivatisierung mit facettenreichen Mischformen namentlich der Public Private Partnerships. Zwar brachte der „Verkauf des Tafelsilbers“ den Kommunen kurzzeitig einen Geldsegen. Doch haben bei weitem nicht alle Privatisierungsmaßnahmen die in sie gesetzten Erwartungen erfüllt und es setzt sich zunehmend die Einsicht durch, dass die Privatwirtschaft nicht zwangsläufig besser, effizienter und kostengünstiger arbeitet als die Öffentliche Hand. Inzwischen deutet sich im kommunalen Bereich eine klare Trendumkehr in Richtung Rekommunalisierung an. Die 17. Fachtagung des Kommunalwissenschaftlichen Instituts (KWI) der Universität Potsdam greift in diese anlaufende Grundsatzdebatte ein und nimmt aktuelle Bestrebungen der Rekommunalisierung lokaler Aufgaben auf. Im Vordergrund stehen erste praktische Erfahrungen, Implementationsprobleme und nicht zuletzt die rechtlichen Rahmenbedingungen und normativen Direktiven für Rekommunalisierungen namentlich in Segmenten der öffentlichen Daseinsvorsorge. N2 - "Private-run better than state-run." Up until a short while ago this had been the battle slogan for a successful discharge of the tight municipal budgets. More and more municipalities chose this supposedly ideal solution. As diverse as the subjects of privatisation are, are its forms to be seen in administrative practice: privatising of property owned by public authorities, of organisations and of responsibilities with various mixed forms specially the Public Private Partnerships. The selling of significant municipal property brought a short welcome sum to the local governments. But by far not all of privatising measures fulfilled expectations and it is increasingly realised that private sector is working neither better, nor more efficiently, nor less expensively than public authorities. Meanwhile a trend towards remunicipalisation is clearly observed in municipal sector. The 17th Congress of the Institute of Local Government Studies of the University of Potsdam (KWI) interferes in this debate on fundamental principles and takes up actual efforts of remunicipalisation. Here, first practical experiences, problems of implementation and last but not least legal basic conditions and normative directives for remunicipalisation specially in parts of the general public service are most important. T3 - KWI-Schriften - 6 KW - Rekommunalisierung KW - öffentliche Daseinsvorsorge KW - Kommunalwirtschaft KW - Privatisierung KW - Konzessionsverträge Y1 - 2012 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-58064 SN - 978-3-86956-170-7 SN - 1867-9528 SN - 1867-951X PB - Universitätsvlerag Potsdam CY - Potsdam ER -