TY  - GEN
A1  - Schwarz, Maria
A1  - Lossow, Kristina
A1  - Kopp, Johannes F.
A1  - Schwerdtle, Tanja
A1  - Kipp, Anna Patricia
T1  - Crosstalk of Nrf2 with the Trace Elements Selenium, Iron, Zinc, and Copper
T2  - Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe
N2  - Trace elements, like Cu, Zn, Fe, or Se, are important for the proper functioning of antioxidant enzymes. However, in excessive amounts, they can also act as pro-oxidants. Accordingly, trace elements influence redox-modulated signaling pathways, such as the Nrf2 pathway. Vice versa, Nrf2 target genes belong to the group of transport and metal binding proteins. In order to investigate whether Nrf2 directly regulates the systemic trace element status, we used mice to study the effect of a constitutive, whole-body Nrf2 knockout on the systemic status of Cu, Zn, Fe, and Se. As the loss of selenoproteins under Se-deprived conditions has been described to further enhance Nrf2 activity, we additionally analyzed the combination of Nrf2 knockout with feeding diets that provide either suboptimal, adequate, or supplemented amounts of Se. Experiments revealed that the Nrf2 knockout partially affected the trace element concentrations of Cu, Zn, Fe, or Se in the intestine, liver, and/or plasma. However, aside from Fe, the other three trace elements were only marginally modulated in an Nrf2-dependent manner. Selenium deficiency mainly resulted in increased plasma Zn levels. One putative mediator could be the metal regulatory transcription factor 1, which was up-regulated with an increasing Se supply and downregulated in Se-supplemented Nrf2 knockout mice.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 1081 
KW  - Nrf2
KW  - selenium
KW  - iron
KW  - copper
KW  - zinc
KW  - homeostasis
Y1  - 2021
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-472873
SN  - 1866-8372
IS  - 1081
ER  - 
TY  - JOUR
A1  - Schwarz, Maria
A1  - Lossow, Kristina
A1  - Kopp, Johannes Florian
A1  - Schwerdtle, Tanja
A1  - Kipp, Anna Patricia
T1  - Crosstalk of Nrf2 with the Trace Elements Selenium, Iron, Zinc, and Copper
JF  - Nutrients
N2  - Trace elements, like Cu, Zn, Fe, or Se, are important for the proper functioning of antioxidant enzymes. However, in excessive amounts, they can also act as pro-oxidants. Accordingly, trace elements influence redox-modulated signaling pathways, such as the Nrf2 pathway. Vice versa, Nrf2 target genes belong to the group of transport and metal binding proteins. In order to investigate whether Nrf2 directly regulates the systemic trace element status, we used mice to study the effect of a constitutive, whole-body Nrf2 knockout on the systemic status of Cu, Zn, Fe, and Se. As the loss of selenoproteins under Se-deprived conditions has been described to further enhance Nrf2 activity, we additionally analyzed the combination of Nrf2 knockout with feeding diets that provide either suboptimal, adequate, or supplemented amounts of Se. Experiments revealed that the Nrf2 knockout partially affected the trace element concentrations of Cu, Zn, Fe, or Se in the intestine, liver, and/or plasma. However, aside from Fe, the other three trace elements were only marginally modulated in an Nrf2-dependent manner. Selenium deficiency mainly resulted in increased plasma Zn levels. One putative mediator could be the metal regulatory transcription factor 1, which was up-regulated with an increasing Se supply and downregulated in Se-supplemented Nrf2 knockout mice.
KW  - Nrf2
KW  - selenium
KW  - iron
KW  - copper
KW  - zinc
KW  - homeostasis
Y1  - 2019
U6  - https://doi.org/10.3390/nu11092112
SN  - 2072-6643
VL  - 11
IS  - 9
PB  - MDPI
CY  - Basel
ER  - 
TY  - JOUR
A1  - Finke, Hannah
A1  - Winkelbeiner, Nicola Lisa
A1  - Lossow, Kristina
A1  - Hertel, Barbara
A1  - Wandt, Viktoria Klara Veronika
A1  - Schwarz, Maria
A1  - Pohl, Gabriele
A1  - Kopp, Johannes Florian
A1  - Ebert, Franziska
A1  - Kipp, Anna Patricia
A1  - Schwerdtle, Tanja
T1  - Effects of a Cumulative, Suboptimal Supply of Multiple Trace Elements in Mice
BT  - trace element status, genomic stability, inflammation, and epigenetics
JF  - Molecular nutrition & food research
N2  - Scope: Trace element (TE) deficiencies often occur accumulated, as nutritional intake is inadequate for several TEs, concurrently. Therefore, the impact of a suboptimal supply of iron, zinc, copper, iodine, and selenium on the TE status, health parameters, epigenetics, and genomic stability in mice are studied. Methods and results: Male mice receive reduced or adequate amounts of TEs for 9 weeks. The TE status is analyzed mass‐spectrometrically in serum and different tissues. Furthermore, gene and protein expression of TE biomarkers are assessed with focus on liver. Iron concentrations are most sensitive toward a reduced supply indicated by increased serum transferrin levels and altered hepatic expression of iron‐related genes. Reduced TE supply results in smaller weight gain but higher spleen and heart weights. Additionally, inflammatory mediators in serum and liver are increased together with hepatic genomic instability. However, global DNA (hydroxy)methylation is unaffected by the TE modulation. Conclusion: Despite homeostatic regulation of most TEs in response to a low intake, this condition still has substantial effects on health parameters. It appears that the liver and immune system react particularly sensitive toward changes in TE intake. The reduced Fe status might be the primary driver for the observed effects.
Y1  - 2020
U6  - https://doi.org/10.1002/mnfr.202000325
SN  - 1613-4125
VL  - 64
IS  - 16
PB  - Wiley-VCH
CY  - Weinheim
ER  -