TY  - JOUR
A1  - Frasca, Stefano
A1  - Milan, Anabel Molero
A1  - Guiet, Amandine
A1  - Goebel, Caren
A1  - Perez-Caballero, Fernando
A1  - Stiba, Konstanze
A1  - Leimkühler, Silke
A1  - Fischer, Anna
A1  - Wollenberger, Ursula
T1  - Bioelectrocatalysis at mesoporous antimony doped tin oxide
 electrodes-Electrochemical characterization and direct enzyme
 communication
JF  - ELECTROCHIMICA ACTA
N2  - In this paper we report immobilization and bioelectrocatalysis of human sulfite oxidase (hSO) on nanostructured antimony doped tin oxide (ATO) thin film electrodes. Two types of ATO thin film electrodes were prepared via evaporation induced self-assembly of ATO nanoparticle sols. The use of a porogen results in different porosity and film thickness. Nevertheless both electrode types reveal similar quasi reversible electrochemical behavior for positive and negatively charged small mediators. Facile and durable immobilization of catalytically active enzyme in a direct electron transfer configuration was achieved without further chemical modification of the ATO surfaces. Interestingly, the binding of hSO onto the ATO surface seems to be not only of electrostatic nature, but also originates from a strong interaction between the histidine-tag of the enzyme and the supporting material. This is suggested from stable sulfite dependent bioelectrocatalytic signals at high ionic strength and imidazole desorption experiments. As such, ATO appears as a promising conductive platform for the immobilization of complex enzymes and their application in bioelectrocatalysis. (C) 2013 Elsevier Ltd. All rights reserved.
KW  - Antimony doped tin dioxide
KW  - Sulfite oxidase
KW  - Direct electrochemistry
KW  - Biosensor
KW  - Bioelectrocatalysis
Y1  - 2013
U6  - https://doi.org/10.1016/j.electacta.2013.03.144
SN  - 0013-4686
SN  - 1873-3859
VL  - 110
IS  - 2
SP  - 172
EP  - 180
PB  - PERGAMON-ELSEVIER SCIENCE LTD
CY  - OXFORD
ER  - 
TY  - JOUR
A1  - Sivanesan, Arumugam
A1  - Kalaivani, Govindasamy
A1  - Fischer, Anna
A1  - Stiba, Konstanze
A1  - Leimkühler, Silke
A1  - Weidinger, Inez M.
T1  - Complementary surface-enhanced resonance raman Spectroscopic Biodetection of mixed protein solutions by Chitosan- and Silica-Coated Plasmon-Tuned Silver Nanoparticles
JF  - Analytical chemistry
N2  - Silver nanoparticles with identical plasmonic properties but different surface functionalities are synthesized and tested as chemically selective surface-enhanced resonance Raman (SERR) amplifiers in a two-component protein solution. The surface plasmon resonances of the particles are tuned to 413 nm to match the molecular resonance of protein heme cofactors. Biocompatible functionalization of the nanoparticles with a thin film of chitosan yields selective SERR enhancement of the anionic protein cytochrome b(5), whereas functionalization with SiO2 amplifies only the spectra of the cationic protein cytochrome c. As a result, subsequent addition of the two differently functionalized particles yields complementary information on the same mixed protein sample solution. Finally, the applicability of chitosan-coated Ag nanoparticles for protein separation was tested by in situ resonance Raman spectroscopy.
Y1  - 2012
U6  - https://doi.org/10.1021/ac301001a
SN  - 0003-2700
VL  - 84
IS  - 13
SP  - 5759
EP  - 5764
PB  - American Chemical Society
CY  - Washington
ER  -