TY  - GEN
A1  - Schmälzlin, Elmar
A1  - Dongen, Joost T. van
A1  - Klimant, Ingo
A1  - Marmodée, Bettina
A1  - Steup, Martin
A1  - Fishahn, Joachim
A1  - Geigenberger, Peter
A1  - Löhmannsröben, Hans-Gerd
T1  - An optical multifrequency phase-modulation method using microbeads for measuring intracellular oxygen concentrations in plants
N2  - A technique has been developed to measure absolute intracellular oxygen concentrations in green plants. Oxygen-sensitive phosphorescent microbeads were injected into the cells and an optical multifrequency phase-modulation technique was used to discriminate the sensor signal from the strong autofluorescence of the plant tissue. The method was established using photosynthesis-competent cells of the giant algae Chara corallina L., and was validated by application to various cell types of other plant species.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - paper 007 
Y1  - 2005
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-12232
ER  - 
TY  - GEN
A1  - Löhmannsröben, Hans-Gerd
A1  - Beck, Michael
A1  - Hildebrandt, Niko
A1  - Schmälzlin, Elmar
A1  - van Dongen, Joost T.
T1  - New challenges in biophotonics : laser-based fluoroimmuno analysis and in-vivo optical oxygen monitoring
N2  - Two examples of our biophotonic research utilizing nanoparticles are presented, namely laser-based fluoroimmuno analysis and in-vivo optical oxygen monitoring. Results of the work include significantly enhanced sensitivity of a homogeneous fluorescence immunoassay and markedly improved spatial resolution of oxygen gradients in root nodules of a legume species.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - paper 018 
KW  - Sauerstoff
KW  - Quantenpunkt
KW  - Lumineszenz
KW  - Immunoassay
KW  - Energietransfer
KW  - Fluoreszenz-Resonanz-Energie-Transfer
KW  - Nanopartikel
KW  - Lanthanoide
KW  - Optode
KW  - Förster Resonanz Energie Transfer
KW  - Biophotonik
KW  - biophotonics
KW  - nanoparticles
KW  - immunoassay
KW  - oxygen
KW  - optode
Y1  - 2006
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-10120
ER  -