TY  - CHAP
A1  - Holzlöhner, Pamela
A1  - Schliebs, Erik
A1  - Maier, Natalia
A1  - Füner, Jonas
A1  - Micheel, Burkhard
A1  - Heilmann, Katja
T1  - Production of monoclonal camelid antibodies by means of hybridoma technology
T2  - The journal of immunology
Y1  - 2013
SN  - 0022-1767
VL  - 190
PB  - American Assoc. of Immunologists
CY  - Bethesda
ER  - 
TY  - JOUR
A1  - Schlör, Anja
A1  - Holzlöhner, Pamela
A1  - Listek, Martin
A1  - Grieß, Cindy
A1  - Butze, Monique
A1  - Micheel, Burkhard
A1  - Hentschel, Christian
A1  - Sowa, Mandy
A1  - Roggenbuck, Dirk
A1  - Schierack, Peter
A1  - Füner, Jonas
A1  - Schliebs, Erik
A1  - Goihl, Alexander
A1  - Reinhold, Dirk
A1  - Hanack, Katja
T1  - Generation and validation of murine monoclonal and camelid recombinant single domain antibodies specific for human pancreatic glycoprotein 2
JF  - New biotechnology
N2  - Pancreatic secretory zymogen-granule membrane glycoprotein 2 (GP2) has been identified as a major autoantigenic target in Crohn’s disease patients. It was reported recently that a long (GP2a) and a short (GP2b) isoform of GP2 exist and that in the outcome of inflammatory bowel diseases (IBD) GP2-specific autoantibodies probably appear as new serological markers for diagnosis and therapeutic monitoring. To investigate this further and in order to establish diagnostic tools for the discrimination of both GP2 isoforms, a set of different murine monoclonal and camelid recombinant single domain antibodies (camelid VHH) was generated and validated in various enzyme-linked immunosorbent assay (ELISA) formats, immunofluorescence on transgenic cell lines and immunohistochemistry on monkey pancreas tissue sections. Out of six binders identified, one was validated as highly specific for GP2a. This murine monoclonal antibody (mAb) was used as capture antibody in construction of a sandwich ELISA for the detection of GP2a. Camelid VHHs or a second murine mAb served as detection antibodies in this system. All antibodies were also able to stain GP2a or GP2b on transgenic cell lines as well as on pancreatic tissue in immunohistochemistry. The KD values measured for the camelid VHHs were between 7 nM and 23pM. This set of specific binders will enable the development of suitable diagnostic tools for GP2-related studies in IBD.
KW  - glycoprotein GP2
KW  - Monoclonal antibodies
KW  - Camelid single domain antibodies
Y1  - 2018
U6  - https://doi.org/10.1016/j.nbt.2018.03.006
SN  - 1871-6784
SN  - 1876-4347
VL  - 45
SP  - 60
EP  - 68
PB  - Elsevier
CY  - Amsterdam
ER  -