TY  - JOUR
A1  - Holdt, Hans-Jürgen
A1  - Müller, Holger
A1  - Kelling, Alexandra
A1  - Drexler, Hans-Joachim
A1  - Müller, Thomas
A1  - Schwarze, Thomas
A1  - Schilde, Uwe
A1  - Starke, Ines
T1  - Mercury(II) chloride and iodide complexes of dithia- and tetrathiacrown ethers
N2  - The complexes [(HgCl2)(2)((ch)(2)30S(4)O(6))] (1), [HgCl,(mn21S(2)O(5))] (2), [HgCl2(ch18S(2)O(4))] (3) and [HgI(meb12S(2)O(2))](2)[Hg2I6] (4) have been synthesized, characterized and their crystal structures were determined. In [(HgCl2)(2)((ch)(2)3OS(4)O(6))] two HgCl2 units are discretely bonded within the ligand cavity of the 30-membered dichinoxaline-tetrathia-30-crown-10 ((ch)(2)30S(4)O(6)) forming a binuclear complex. HgCl2 forms I : I "in-cavity" complexes with the 21-membered maleonitrile-dithia-21-crown-7(mn21S(2)O(5)) ligand and the 18-membered chinoxaline- dithia-18-crown-6 (ch18S(2)O(4)) ligand, respectively. The 12-membered 4-methyl-benzo-dithia-12-crown-4 (meb12S(2)O(2)) ligand gave with two equivalents HgI2 the compound [HgI(meb12S(2)O(2))](2)[Hg2I6]. In the cation [HgI(meb12S(2)O(2))](+) meb12S(2)O(2) forms with the cation HgI+ a half-sandwich complex
Y1  - 2006
UR  - http://www3.interscience.wiley.com/cgi-bin/jhome/10005159
U6  - https://doi.org/10.1002/zaac.200500281
SN  - 0044-2313
ER  - 
TY  - JOUR
A1  - Warrington, Nicole
A1  - Beaumont, Robin
A1  - Horikoshi, Momoko
A1  - Day, Felix R.
A1  - Helgeland, Øyvind
A1  - Laurin, Charles
A1  - Bacelis, Jonas
A1  - Peng, Shouneng
A1  - Hao, Ke
A1  - Feenstra, Bjarke
A1  - Wood, Andrew R.
A1  - Mahajan, Anubha
A1  - Tyrrell, Jessica
A1  - Robertson, Neil R.
A1  - Rayner, N. William
A1  - Qiao, Zhen
A1  - Moen, Gunn-Helen
A1  - Vaudel, Marc
A1  - Marsit, Carmen
A1  - Chen, Jia
A1  - Nodzenski, Michael
A1  - Schnurr, Theresia M.
A1  - Zafarmand, Mohammad Hadi
A1  - Bradfield, Jonathan P.
A1  - Grarup, Niels
A1  - Kooijman, Marjolein N.
A1  - Li-Gao, Ruifang
A1  - Geller, Frank
A1  - Ahluwalia, Tarunveer Singh
A1  - Paternoster, Lavinia
A1  - Rueedi, Rico
A1  - Huikari, Ville
A1  - Hottenga, Jouke-Jan
A1  - Lyytikäinen, Leo-Pekka
A1  - Cavadino, Alana
A1  - Metrustry, Sarah
A1  - Cousminer, Diana L.
A1  - Wu, Ying
A1  - Thiering, Elisabeth Paula
A1  - Wang, Carol A.
A1  - Have, Christian Theil
A1  - Vilor-Tejedor, Natalia
A1  - Joshi, Peter K.
A1  - Painter, Jodie N.
A1  - Ntalla, Ioanna
A1  - Myhre, Ronny
A1  - Pitkänen, Niina
A1  - van Leeuwen, Elisabeth M.
A1  - Joro, Raimo
A1  - Lagou, Vasiliki
A1  - Richmond, Rebecca C.
A1  - Espinosa, Ana
A1  - Barton, Sheila J.
A1  - Inskip, Hazel M.
A1  - Holloway, John W.
A1  - Santa-Marina, Loreto
A1  - Estivill, Xavier
A1  - Ang, Wei
A1  - Marsh, Julie A.
A1  - Reichetzeder, Christoph
A1  - Marullo, Letizia
A1  - Hocher, Berthold
A1  - Lunetta, Kathryn L.
A1  - Murabito, Joanne M.
A1  - Relton, Caroline L.
A1  - Kogevinas, Manolis
A1  - Chatzi, Leda
A1  - Allard, Catherine
A1  - Bouchard, Luigi
A1  - Hivert, Marie-France
A1  - Zhang, Ge
A1  - Muglia, Louis J.
A1  - Heikkinen, Jani
A1  - Morgen, Camilla S.
A1  - van Kampen, Antoine H. C.
A1  - van Schaik, Barbera D. C.
A1  - Mentch, Frank D.
A1  - Langenberg, Claudia
A1  - Scott, Robert A.
A1  - Zhao, Jing Hua
A1  - Hemani, Gibran
A1  - Ring, Susan M.
A1  - Bennett, Amanda J.
A1  - Gaulton, Kyle J.
A1  - Fernandez-Tajes, Juan
A1  - van Zuydam, Natalie R.
A1  - Medina-Gomez, Carolina
A1  - de Haan, Hugoline G.
A1  - Rosendaal, Frits R.
A1  - Kutalik, Zoltán
A1  - Marques-Vidal, Pedro
A1  - Das, Shikta
A1  - Willemsen, Gonneke
A1  - Mbarek, Hamdi
A1  - Müller-Nurasyid, Martina
A1  - Standl, Marie
A1  - Appel, Emil V. R.
A1  - Fonvig, Cilius Esmann
A1  - Trier, Caecilie
A1  - van Beijsterveldt, Catharina E. M.
A1  - Murcia, Mario
A1  - Bustamante, Mariona
A1  - Bonàs-Guarch, Sílvia
A1  - Hougaard, David M.
A1  - Mercader, Josep M.
A1  - Linneberg, Allan
A1  - Schraut, Katharina E.
A1  - Lind, Penelope A.
A1  - Medland, Sarah Elizabeth
A1  - Shields, Beverley M.
A1  - Knight, Bridget A.
A1  - Chai, Jin-Fang
A1  - Panoutsopoulou, Kalliope
A1  - Bartels, Meike
A1  - Sánchez, Friman
A1  - Stokholm, Jakob
A1  - Torrents, David
A1  - Vinding, Rebecca K.
A1  - Willems, Sara M.
A1  - Atalay, Mustafa
A1  - Chawes, Bo L.
A1  - Kovacs, Peter
A1  - Prokopenko, Inga
A1  - Tuke, Marcus A.
A1  - Yaghootkar, Hanieh
A1  - Ruth, Katherine S.
A1  - Jones, Samuel E.
A1  - Loh, Po-Ru
A1  - Murray, Anna
A1  - Weedon, Michael N.
A1  - Tönjes, Anke
A1  - Stumvoll, Michael
A1  - Michaelsen, Kim Fleischer
A1  - Eloranta, Aino-Maija
A1  - Lakka, Timo A.
A1  - van Duijn, Cornelia M.
A1  - Kiess, Wieland
A1  - Koerner, Antje
A1  - Niinikoski, Harri
A1  - Pahkala, Katja
A1  - Raitakari, Olli T.
A1  - Jacobsson, Bo
A1  - Zeggini, Eleftheria
A1  - Dedoussis, George V.
A1  - Teo, Yik-Ying
A1  - Saw, Seang-Mei
A1  - Montgomery, Grant W.
A1  - Campbell, Harry
A1  - Wilson, James F.
A1  - Vrijkotte, Tanja G. M.
A1  - Vrijheid, Martine
A1  - de Geus, Eco J. C. N.
A1  - Hayes, M. Geoffrey
A1  - Kadarmideen, Haja N.
A1  - Holm, Jens-Christian
A1  - Beilin, Lawrence J.
A1  - Pennell, Craig E.
A1  - Heinrich, Joachim
A1  - Adair, Linda S.
A1  - Borja, Judith B.
A1  - Mohlke, Karen L.
A1  - Eriksson, Johan G.
A1  - Widen, Elisabeth E.
A1  - Hattersley, Andrew T.
A1  - Spector, Tim D.
A1  - Kaehoenen, Mika
A1  - Viikari, Jorma S.
A1  - Lehtimaeki, Terho
A1  - Boomsma, Dorret I.
A1  - Sebert, Sylvain
A1  - Vollenweider, Peter
A1  - Sorensen, Thorkild I. A.
A1  - Bisgaard, Hans
A1  - Bonnelykke, Klaus
A1  - Murray, Jeffrey C.
A1  - Melbye, Mads
A1  - Nohr, Ellen A.
A1  - Mook-Kanamori, Dennis O.
A1  - Rivadeneira, Fernando
A1  - Hofman, Albert
A1  - Felix, Janine F.
A1  - Jaddoe, Vincent W. V.
A1  - Hansen, Torben
A1  - Pisinger, Charlotta
A1  - Vaag, Allan A.
A1  - Pedersen, Oluf
A1  - Uitterlinden, Andre G.
A1  - Jarvelin, Marjo-Riitta
A1  - Power, Christine
A1  - Hypponen, Elina
A1  - Scholtens, Denise M.
A1  - Lowe, William L.
A1  - Smith, George Davey
A1  - Timpson, Nicholas J.
A1  - Morris, Andrew P.
A1  - Wareham, Nicholas J.
A1  - Hakonarson, Hakon
A1  - Grant, Struan F. A.
A1  - Frayling, Timothy M.
A1  - Lawlor, Debbie A.
A1  - Njolstad, Pal R.
A1  - Johansson, Stefan
A1  - Ong, Ken K.
A1  - McCarthy, Mark I.
A1  - Perry, John R. B.
A1  - Evans, David M.
A1  - Freathy, Rachel M.
T1  - Maternal and fetal genetic effects on birth weight and their relevance to cardio-metabolic risk factors
JF  - Nature genetics
N2  - Birth weight variation is influenced by fetal and maternal genetic and non-genetic factors, and has been reproducibly associated with future cardio-metabolic health outcomes. In expanded genome-wide association analyses of own birth weight (n = 321,223) and offspring birth weight (n = 230,069 mothers), we identified 190 independent association signals (129 of which are novel). We used structural equation modeling to decompose the contributions of direct fetal and indirect maternal genetic effects, then applied Mendelian randomization to illuminate causal pathways. For example, both indirect maternal and direct fetal genetic effects drive the observational relationship between lower birth weight and higher later blood pressure: maternal blood pressure-raising alleles reduce offspring birth weight, but only direct fetal effects of these alleles, once inherited, increase later offspring blood pressure. Using maternal birth weight-lowering genotypes to proxy for an adverse intrauterine environment provided no evidence that it causally raises offspring blood pressure, indicating that the inverse birth weight-blood pressure association is attributable to genetic effects, and not to intrauterine programming.
Y1  - 2019
SN  - 1061-4036
SN  - 1546-1718
VL  - 51
IS  - 5
SP  - 804
EP  - +
PB  - Nature Publ. Group
CY  - New York
ER  - 
TY  - JOUR
A1  - Laub, Julian
A1  - Roth, Volker
A1  - Buhmann, Joachim
A1  - Müller, Klaus-Robert
T1  - On the information and representation of non-Euclidean pairwise data
N2  - Two common data representations are mostly used in intelligent data analysis, namely the vectorial and the pairwise representation. Pairwise data which satisfy the restrictive conditions of Euclidean spaces can be faithfully translated into a Euclidean vectorial representation by embedding. Non-metric pairwise data with violations of symmetry, reflexivity or triangle inequality pose a substantial conceptual problem for pattern recognition since the amount of predictive structural information beyond what can be measured by embeddings is unclear. We show by systematic modeling of non-Euclidean pairwise data that there exists metric violations which can carry valuable problem specific information. Furthermore, Euclidean and non-metric data can be unified on the level of structural information contained in the data. Stable component analysis selects linear subspaces which are particularly insensitive to data fluctuations. Experimental results from different domains support our pattern recognition strategy.
Y1  - 2006
UR  - http://www.sciencedirect.com/science/journal/00313203
U6  - https://doi.org/10.1016/j.patcog.2006.04.016
SN  - 0031-3203
ER  - 
TY  - JOUR
A1  - Ludwig, Joachim
A1  - Müller, Kurt R.
T1  - Kompetenzentwicklung im Interessenfeld betrieblicher Modernisierung. Fallarbeit als Konzept zur Kompetenzentwicklung?
Y1  - 2004
ER  - 
TY  - GEN
A1  - Ludwig, Joachim
A1  - Müller, Kurt R.
T1  - Kompetenzentwicklung im Spannungsfeld betrieblicher Modernisierung : 'Fallarbeit' als Konzept zur Kompetenzentwicklung?
N2  - Inhalt: 1 Entwicklung der Fragestellung 2. Erscheinungsformen betrieblicher Modernisierung – Kompetenzentwicklung als interessiertes Handeln 3. Die Managementperspektive auf Kompetenzentwicklung – ‚Entwickeln’ als steuern und trainieren 4. Die Subjektperspektive auf Kompetenzentwicklung – ‚Entwickeln’ als sich verständigen, sich entfalten - Die lerntheoretische Rahmung der Subjektperspektive - ‚Fallarbeit’ als empirischer und verstehender Zugang zu subjektiven Selbstverständigungsprozessen (1) Fallerzählung und Nachfragen (2) Spuren suchen und Kernthemen finden (3) Kernthemen bearbeiten
T3  - Zweitveröffentlichungen der Universität Potsdam : Humanwissenschaftliche Reihe - paper 011 
Y1  - 2004
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-12676
ER  - 
TY  - JOUR
A1  - Wöhl-Bruhn, Stefanie
A1  - Badar, Muhammad
A1  - Bertz, Andreas
A1  - Tiersch, Brigitte
A1  - Koetz, Joachim
A1  - Menzel, Henning
A1  - Müller, Peter P.
A1  - Bunjes, Heike
T1  - Comparison of in vitro and in vivo protein release from hydrogel systems
JF  - Journal of controlled release
N2  - Hydrogel systems based on hydroxyethyl starch-polyethylene glycol methacrylate (HES-P(EG)(6)MA) or hydroxyethyl starch methacrylate (HES-MA) were used to assess the protein release behavior. Here, we analyzed the in vitro release of FITC-anti-human antibodies incorporated in either HES-P(EG)(6)MA or HES-MA hydrogel delivery systems in PBS or human serum. In addition, hydrogel disks and microparticles prepared from the two polymers were subcutaneously implanted in BALB/c mice. The in vivo release of FITC-IgG was non-invasively monitored by an in vivo imaging system (IVIS 200) over a time period of up to 3 months. The imaging system allowed to asses individual animals over time, therefore only a small number of animals was required to obtain high quality data. The reduction in fluorescence intensity at the site of administration was compared to in vitro release profiles. These investigations demonstrated a sustained release from HES-MA hydrogel disks compared to rapidly degrading HES-P(EG)(6)MA disks and microparticles. The sustained release from HES-MA disks could be further optimized by using increased polymer concentrations. Human serum as in vitro release medium reflected better the in vivo release from HES-P(EG)(6)MA systems than PBS, suggesting that the presence of organic substances like proteins or lipids may play a significant role for the release kinetics.
KW  - In vivo imaging system (IVIS)
KW  - Hydrogel disks
KW  - Hydrogel microparticles
KW  - Release
KW  - In vivo-in vitro correlation
KW  - Hydroxyethyl starch (HES)
Y1  - 2012
U6  - https://doi.org/10.1016/j.jconrel.2012.05.049
SN  - 0168-3659
VL  - 162
IS  - 1
SP  - 127
EP  - 133
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Mikelskis, Helmut F.
A1  - Böschen, Kerstin
A1  - Müller, Sabine Lenore
A1  - Gessinger, Joachim
A1  - Grözinger, Karl Erich
A1  - Gründel, Olaf
A1  - Drexler, Peter
A1  - Hassler, Gerda
A1  - Klinger, Sönke
A1  - Scherwath, Julia
A1  - Buhl, Claudia M.
A1  - Böwe, Heiko
A1  - Romeike, Ralf
A1  - Schwill, Andreas
T1  - Portal = Betrachtet: Profilbereich untersucht Kulturen im Vergleich
BT  - Die Potsdamer Universitätszeitung
N2  - Aus dem Inhalt:
- Betrachtet: Profilbereich untersucht Kulturen im Vergleich
- Frauenförderung mit mehr Erfolg
- AStA mit neuem Profil
- Universitätsverlag mit eigenem Know-how
T3  - Portal: Das Potsdamer Universitätsmagazin - 11-12/2003 
Y1  - 2003
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-439776
SN  - 1618-6893
IS  - 11-12/2003
ER  - 
TY  - JOUR
A1  - Ast, Sandra
A1  - Schwarze, Thomas
A1  - Müller, Holger
A1  - Sukhanov, Aleksey
A1  - Michaelis, Stefanie
A1  - Wegener, Joachim
A1  - Wolfbeis, Otto S.
A1  - Körzdörfer, Thomas
A1  - Dürkop, Axel
A1  - Holdt, Hans-Jürgen
T1  - A highly K+-Selective Phenylaza-[18]crown-6-Lariat-Ether-Based Fluoroionophore and its application in the sensing of K+ Ions with an optical sensor film and in cells
JF  - Chemistry - a European journal
N2  - Herein, we report the synthesis of two phenylaza-[18]crown-6 lariat ethers with a coumarin fluorophore (1 and 2) and we reveal that compound 1 is an excellent probe for K+ ions under simulated physiological conditions. The presence of a 2-methoxyethoxy lariat group at the ortho position of the anilino moiety is crucial to the substantially increased stability of compounds 1 and 2 over their lariat-free phenylaza-[18] crown-6 ether analogues. Probe 1 shows a high K+/Na+ selectivity and a 2.5-fold fluorescence enhancement was observed in the presence of 100 mm K+ ions. A fluorescent membrane sensor, which was prepared by incorporating probe 1 into a hydrogel, showed a fully reversible response, a response time of 150 s, and a signal change of 7.8% per 1 mm K+ within the range 1-10 mm K+. The membrane was easily fabricated (only a single sensing layer on a solid polyester support), yet no leaching was observed. Moreover, compound 1 rapidly permeated into cells, was cytocompatible, and was suitable for the fluorescent imaging of K+ ions on both the extracellular and intracellular levels.
KW  - crown compounds
KW  - fluorescence
KW  - gels
KW  - potassium
KW  - sensors
Y1  - 2013
U6  - https://doi.org/10.1002/chem.201302350
SN  - 0947-6539
SN  - 1521-3765
VL  - 19
IS  - 44
SP  - 14911
EP  - 14917
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Partenheimer, Lutz
A1  - Müller, Joachim
T1  - Der 11. Juni 1157 und seine Bedeutung für Stadt und Land Brandenburg : Versuch einer Würdigung zum 850. "Geburtsjahr" der Mark Brandenburg aus archäologischer und historischer Sicht
Y1  - 2008
ER  - 
TY  - JOUR
A1  - Ludwig, Joachim
A1  - Müller, Katja
T1  - Forschungsstand zu Alphabetisierung und Grundbildung
Y1  - 2012
SN  - 978-3-7639-5067-6
ER  - 
TY  - JOUR
A1  - Bauer, Joachim
A1  - Stamm, Axel
A1  - Virnich, Katharina
A1  - Wissing, Karen
A1  - Müller, Udo
A1  - Wirsching, Michael
A1  - Schaarschmidt, Uwe
T1  - Correlation between burnout syndrome and psychological and psychosomatic symptoms among teachers
N2  - Objectives: Psychosomatic disorders and symptoms that correlate with the so-called burnout syndrome turned out to be the main cause of increasing rates of premature retirement of school teachers. The aim of this study was to evaluate the relation between occupational burden and psychological strain of teachers who are still in work. Methods: A sample of 408 teachers at ten grammar schools (am: High school; German: Gymnasium) in south-western Germany was evaluated. To determine the styles of coping with occupational burden we used the measure of coping capacity questionnaire (MECCA). To analyse the psychopathological and psychosomatic symptom load we applied SCL 90 R questionnaire. Results: According to the MECCA questionnaire, 32.5% of the sample suffered from burnout (type B), 17.7% suffered severe strain (type A), 35.9% showed an unambitious (type S) and 13.8% showed a healthy-ambitious coping style (type G). Burnout was significantly higher among women, divorced teachers and teachers working part-time. As part of the MECCA, teachers were asked to rate what they regarded as the strongest factor resulting in occupational burden. Teachers indicated that, besides high numbers of pupils in one class, they regarded destructive and aggressive behaviour of pupils as the primary stress factor. According to the SCL 90 R, 20% of the sample showed a severe degree (defined as > 70 points in the SCL90R GSI) of psychological and psychosomatic symptoms. MECCA type B (burnout) correlated significantly with high psychological and psychosomatic symptom load according to the SCL90R. Conclusions: In school teachers, burnout syndrome, a construct that derived from occupational psychology and occupational medicine, is significantly correlated with psychological and psychosomatic symptoms. Teachers rate destructive and aggressive behaviour of pupils as the primary stress factor.
Y1  - 2006
UR  - http://www.springerlink.com/content/101165
U6  - https://doi.org/10.1007/s00420-005-0050-y
SN  - 0340-0131
ER  - 
TY  - JOUR
A1  - Broscheit, Frank
A1  - Bütow, Martin
A1  - Englert, Wolfgang
A1  - Jahn, Gert
A1  - Klohn, Werner
A1  - Knitschky, Wolfgang
A1  - Reinecke, Hans-Joachim
A1  - Müller, Helmut
A1  - Reinhardt, Karl Heinz
A1  - Schmidt, Marianne
A1  - Schmidtke, Kurt-Dietmar
A1  - Schöpflin, F.
A1  - Starke, Rainer
A1  - Vahldiek, B. W.
A1  - Wehrs, Klaus
A1  - Wetzel, Jürgen
T1  - Seydlitz Erdkunde 2
Y1  - 1996
PB  - Schroedel
CY  - Hannover
ER  - 
TY  - JOUR
A1  - Skirycz, Aleksandra
A1  - Reichelt, Michael
A1  - Burow, Meike
A1  - Birkemeyer, Claudia Sabine
A1  - Rolcik, Jacub
A1  - Kopka, Joachim
A1  - Zanor, Maria Ines
A1  - Gershenzon, Jonathan
A1  - Strnad, Miroslav
A1  - Szopa, Jan
A1  - Müller-Röber, Bernd
A1  - Witt, Isabell
T1  - DOF transcription factor AtDof1.1 (OBP2) is part of a regulatory network controlling glucosinolate biosynthesis in Arabidopsis
N2  - Glucosinolates are a group of secondary metabolites that function as defense substances against herbivores and micro-organisms in the plant order Capparales. Indole glucosinolates (IGS), derivatives of tryptophan, may also influence plant growth and development. In Arabidopsis thaliana, indole-3-acetaldoxime (IAOx) produced from tryptophan by the activity of two cytochrome P450 enzymes, CYP79B2 and CYP79B3, serves as a precursor for IGS biosynthesis but is also an intermediate in the biosynthetic pathway of indole-3-acetic acid (IAA). Another cytochrome P450 enzyme, CYP83B1, funnels IAOx into IGS. Although there is increasing information about the genes involved in this biochemical pathway, their regulation is not fully understood. OBP2 has recently been identified as a member of the DNA-binding-with-one- finger (DOF) transcription factors, but its function has not been studied in detail so far. Here we report that OBP2 is expressed in the vasculature of all Arabidopsis organs, including leaves, roots, flower stalks and petals. OBP2 expression is induced in response to a generalist herbivore, Spodoptera littoralis, and by treatment with the plant signalling molecule methyl jasmonate, both of which also trigger IGS accumulation. Constitutive and inducible over- expression of OBP2 activates expression of CYP83B1. In addition, auxin concentration is increased in leaves and seedlings of OBP2 over-expression lines relative to wild-type, and plant size is diminished due to a reduction in cell size. RNA interference-mediated OBP2 blockade leads to reduced expression of CYP83B1. Collectively, these data provide evidence that OBP2 is part of a regulatory network that regulates glucosinolate biosynthesis in Arabidopsis
Y1  - 2006
UR  - http://onlinelibrary.wiley.com/doi/10.1111/j.1365-313X.2006.02767.x/full
ER  - 
TY  - JOUR
A1  - Rauf, Mamoona
A1  - Arif, Muhammad
A1  - Fisahn, Joachim
A1  - Xue, Gang-Ping
A1  - Balazadeh, Salma
A1  - Müller-Röber, Bernd
T1  - NAC transcription factor speedy hyponastic growth regulates flooding-induced leaf movement in arabidopsis
JF  - The plant cell
N2  - In rosette plants, root flooding (waterlogging) triggers rapid upward (hyponastic) leaf movement representing an important architectural stress response that critically determines plant performance in natural habitats. The directional growth is based on localized longitudinal cell expansion at the lower (abaxial) side of the leaf petiole and involves the volatile phytohormone ethylene (ET). We report the existence of a transcriptional core unit underlying directional petiole growth in Arabidopsis thaliana, governed by the NAC transcription factor SPEEDY HYPONASTIC GROWTH (SHYG). Overexpression of SHYG in transgenic Arabidopsis thaliana enhances waterlogging-triggered hyponastic leaf movement and cell expansion in abaxial cells of the basal petiole region, while both responses are largely diminished in shyg knockout mutants. Expression of several EXPANSIN and XYLOGLUCAN ENDOTRANSGLYCOSYLASE/HYDROLASE genes encoding cell wall-loosening proteins was enhanced in SHYG overexpressors but lowered in shyg. We identified ACC OXIDASE5 (ACO5), encoding a key enzyme of ET biosynthesis, as a direct transcriptional output gene of SHYG and found a significantly reduced leaf movement in response to root flooding in aco5 T-DNA insertion mutants. Expression of SHYG in shoot tissue is triggered by root flooding and treatment with ET, constituting an intrinsic ET-SHYG-ACO5 activator loop for rapid petiole cell expansion upon waterlogging.
Y1  - 2013
U6  - https://doi.org/10.1105/tpc.113.117861
SN  - 1040-4651
SN  - 1532-298X
VL  - 25
IS  - 12
SP  - 4941
EP  - 4955
PB  - American Society of Plant Physiologists
CY  - Rockville
ER  - 
TY  - JOUR
A1  - Müller, Sandra Marie
A1  - Ebert, Franziska
A1  - Bornhorst, Julia
A1  - Galla, Hans-Joachim
A1  - Francesconi, Kevin A.
A1  - Schwerdtle, Tanja
T1  - Arsenic-containing hydrocarbons disrupt a model in vitro blood-cerebrospinal fluid barrier
JF  - Journal of trace elements in medicine and biology
N2  - Lipid-soluble arsenicals, so-called arsenolipids, have gained a lot of attention in the last few years because of their presence in many seafoods and reports showing substantial cytotoxicity emanating from arsenic-containing hydrocarbons (AsHCs), a prominent subgroup of the arsenolipids. More recent in vivo and in vitro studies indicate that some arsenolipids might have adverse effects on brain health. In the present study, we focused on the effects of selected arsenolipids and three representative metabolites on the blood-cerebrospinal fluid barrier (B-CSF-B), a brain-regulating interface. For this purpose, we incubated an in vitro model of the B-CSF-B composed of porcine choroid plexus epithelial cells (PCPECs) with three AsHCs, two arsenic-containing fatty acids (AsFAs) and three representative arsenolipid metabolites (dimethylarsinic acid, thio/oxo-dimethylpropanoic acid) to examine their cytotoxic potential and impact on barrier integrity. The toxic arsenic species arsenite was also tested in this way and served as a reference substance. While AsFAs and the metabolites showed no cytotoxic effects in the conducted assays, AsHCs showed a strong cytotoxicity, being up to 1.5-fold more cytotoxic than arsenite. Analysis of the in vitro B-CSF-B integrity showed a concentration dependent disruption of the barrier within 72 h. The correlation with the decreased plasma membrane surface area (measured as capacitance) indicates cytotoxic effects. These findings suggest exposure to elevated levels of certain arsenolipids may have detrimental consequences for the central nervous system.
KW  - Arsenolipids
KW  - Blood-liquor barrier
KW  - Blood-cerebrospinal fluid barrier
KW  - Arsenic-containing hydrocarbons
KW  - Arsenic-containing fatty acids
Y1  - 2018
U6  - https://doi.org/10.1016/j.jtemb.2018.01.020
SN  - 0946-672X
VL  - 49
SP  - 171
EP  - 177
PB  - Elsevier GMBH
CY  - München
ER  - 
TY  - JOUR
A1  - Müller, Sandra Marie
A1  - Ebert, Franziska
A1  - Raber, Georg
A1  - Meyer, Sören
A1  - Bornhorst, Julia
A1  - Hüwel, Stephan
A1  - Galla, Hans-Joachim
A1  - Francesconi, Kevin A.
A1  - Schwerdtle, Tanja
T1  - Effects of arsenolipids on in vitro blood-brain barrier model
JF  - Archives of toxicology : official journal of EUROTOX
N2  - Arsenic-containing hydrocarbons (AsHCs), a subgroup of arsenolipids (AsLs) occurring in fish and edible algae, possess a substantial neurotoxic potential in fully differentiated human brain cells. Previous in vivo studies indicating that AsHCs cross the blood–brain barrier of the fruit fly Drosophila melanogaster raised the question whether AsLs could also cross the vertebrate blood–brain barrier (BBB). In the present study, we investigated the impact of several representatives of AsLs (AsHC 332, AsHC 360, AsHC 444, and two arsenic-containing fatty acids, AsFA 362 and AsFA 388) as well as of their metabolites (thio/oxo-dimethylpropionic acid, dimethylarsinic acid) on porcine brain capillary endothelial cells (PBCECs, in vitro model for the blood–brain barrier). AsHCs exerted the strongest cytotoxic effects of all investigated arsenicals as they were up to fivefold more potent than the toxic reference species arsenite (iAsIII). In our in vitro BBB-model, we observed a slight transfer of AsHC 332 across the BBB after 6 h at concentrations that do not affect the barrier integrity. Furthermore, incubation with AsHCs for 72 h led to a disruption of the barrier at sub-cytotoxic concentrations. The subsequent immunocytochemical staining of three tight junction proteins revealed a significant impact on the cell membrane. Because AsHCs enhance the permeability of the in vitro blood–brain barrier, a similar behavior in an in vivo system cannot be excluded. Consequently, AsHCs might facilitate the transfer of accompanying foodborne toxicants into the brain.
KW  - Arsenolipids
KW  - Arsenic-containing hydrocarbons
KW  - Arsenic-containing fatty acids
KW  - In vitro blood-brain barrier model
Y1  - 2017
SN  - 0340-5761
SN  - 1432-0738
VL  - 92
IS  - 2
SP  - 823
EP  - 832
PB  - Springer
CY  - Heidelberg
ER  - 
TY  - JOUR
A1  - Mettler, Tabea
A1  - Mühlhaus, Timo
A1  - Hemme, Dorothea
A1  - Schöttler, Mark Aurel
A1  - Rupprecht, Jens
A1  - Idoine, Adam
A1  - Veyel, Daniel
A1  - Pal, Sunil Kumar
A1  - Yaneva-Roder, Liliya
A1  - Winck, Flavia Vischi
A1  - Sommer, Frederik
A1  - Vosloh, Daniel
A1  - Seiwert, Bettina
A1  - Erban, Alexander
A1  - Burgos, Asdrubal
A1  - Arvidsson, Samuel Janne
A1  - Schoenfelder, Stephanie
A1  - Arnold, Anne
A1  - Guenther, Manuela
A1  - Krause, Ursula
A1  - Lohse, Marc
A1  - Kopka, Joachim
A1  - Nikoloski, Zoran
A1  - Müller-Röber, Bernd
A1  - Willmitzer, Lothar
A1  - Bock, Ralph
A1  - Schroda, Michael
A1  - Stitt, Mark
T1  - Systems analysis of the response of photosynthesis, metabolism, and growth to an increase in irradiance in the photosynthetic model organism chlamydomonas reinhardtii
JF  - The plant cell
N2  - We investigated the systems response of metabolism and growth after an increase in irradiance in the nonsaturating range in the algal model Chlamydomonas reinhardtii. In a three-step process, photosynthesis and the levels of metabolites increased immediately, growth increased after 10 to 15 min, and transcript and protein abundance responded by 40 and 120 to 240 min, respectively. In the first phase, starch and metabolites provided a transient buffer for carbon until growth increased. This uncouples photosynthesis from growth in a fluctuating light environment. In the first and second phases, rising metabolite levels and increased polysome loading drove an increase in fluxes. Most Calvin-Benson cycle (CBC) enzymes were substrate-limited in vivo, and strikingly, many were present at higher concentrations than their substrates, explaining how rising metabolite levels stimulate CBC flux. Rubisco, fructose-1,6-biosphosphatase, and seduheptulose-1,7-bisphosphatase were close to substrate saturation in vivo, and flux was increased by posttranslational activation. In the third phase, changes in abundance of particular proteins, including increases in plastidial ATP synthase and some CBC enzymes, relieved potential bottlenecks and readjusted protein allocation between different processes. Despite reasonable overall agreement between changes in transcript and protein abundance (R-2 = 0.24), many proteins, including those in photosynthesis, changed independently of transcript abundance.
Y1  - 2014
U6  - https://doi.org/10.1105/tpc.114.124537
SN  - 1040-4651
SN  - 1532-298X
VL  - 26
IS  - 6
SP  - 2310
EP  - 2350
PB  - American Society of Plant Physiologists
CY  - Rockville
ER  - 
TY  - JOUR
A1  - Schmidt, Romy
A1  - Mieulet, Delphine
A1  - Hubberten, Hans-Michael
A1  - Obata, Toshihiro
A1  - Höfgen, Rainer
A1  - Fernie, Alisdair R.
A1  - Fisahn, Joachim
A1  - Segundo, Blanca San
A1  - Guiderdoni, Emmanuel
A1  - Schippers, Jos H. M.
A1  - Müller-Röber, Bernd
T1  - Salt-responsive ERF1 regulates reactive oxygen species-dependent signaling during the initial response to salt stress in rice
JF  - The plant cell
N2  - Early detection of salt stress is vital for plant survival and growth. Still, the molecular processes controlling early salt stress perception and signaling are not fully understood. Here, we identified SALT-RESPONSIVE ERF1 (SERF1), a rice (Oryza sativa) transcription factor (TF) gene that shows a root-specific induction upon salt and hydrogen peroxide (H2O2) treatment. Loss of SERF1 impairs the salt-inducible expression of genes encoding members of a mitogen-activated protein kinase (MAPK) cascade and salt tolerance-mediating TFs. Furthermore, we show that SERF1-dependent genes are H2O2 responsive and demonstrate that SERF1 binds to the promoters of MAPK KINASE KINASE6 (MAP3K6), MAPK5, DEHYDRATION-RESPONSIVE ELEMENT BINDING2A (DREB2A), and ZINC FINGER PROTEIN179 (ZFP179) in vitro and in vivo. SERF1 also directly induces its own gene expression. In addition, SERF1 is a phosphorylation target of MAPK5, resulting in enhanced transcriptional activity of SERF1 toward its direct target genes. In agreement, plants deficient for SERF1 are more sensitive to salt stress compared with the wild type, while constitutive overexpression of SERF1 improves salinity tolerance. We propose that SERF1 amplifies the reactive oxygen species-activated MAPK cascade signal during the initial phase of salt stress and translates the salt-induced signal into an appropriate expressional response resulting in salt tolerance.
Y1  - 2013
U6  - https://doi.org/10.1105/tpc.113.113068
SN  - 1040-4651
VL  - 25
IS  - 6
SP  - 2115
EP  - 2131
PB  - American Society of Plant Physiologists
CY  - Rockville
ER  - 
TY  - JOUR
A1  - Watanabe, Mutsumi
A1  - Balazadeh, Salma
A1  - Tohge, Takayuki
A1  - Erban, Alexander
A1  - Giavalisco, Patrick
A1  - Kopka, Joachim
A1  - Müller-Röber, Bernd
A1  - Fernie, Alisdair R.
A1  - Höfgen, Rainer
T1  - Comprehensive dissection of spatiotemporal metabolic shifts in primary, secondary, and lipid metabolism during developmental senescence in arabidopsis
JF  - Plant physiology : an international journal devoted to physiology, biochemistry, cellular and molecular biology, biophysics and environmental biology of plants
N2  - Developmental senescence is a coordinated physiological process in plants and is critical for nutrient redistribution from senescing leaves to newly formed sink organs, including young leaves and developing seeds. Progress has been made concerning the genes involved and the regulatory networks controlling senescence. The resulting complex metabolome changes during senescence have not been investigated in detail yet. Therefore, we conducted a comprehensive profiling of metabolites, including pigments, lipids, sugars, amino acids, organic acids, nutrient ions, and secondary metabolites, and determined approximately 260 metabolites at distinct stages in leaves and siliques during senescence in Arabidopsis (Arabidopsis thaliana). This provided an extensive catalog of metabolites and their spatiotemporal cobehavior with progressing senescence. Comparison with silique data provides clues to source-sink relations. Furthermore, we analyzed the metabolite distribution within single leaves along the basipetal sink-source transition trajectory during senescence. Ceramides, lysolipids, aromatic amino acids, branched chain amino acids, and stress-induced amino acids accumulated, and an imbalance of asparagine/aspartate, glutamate/glutamine, and nutrient ions in the tip region of leaves was detected. Furthermore, the spatiotemporal distribution of tricarboxylic acid cycle intermediates was already changed in the presenescent leaves, and glucosinolates, raffinose, and galactinol accumulated in the base region of leaves with preceding senescence. These results are discussed in the context of current models of the metabolic shifts occurring during developmental and environmentally induced senescence. As senescence processes are correlated to crop yield, the metabolome data and the approach provided here can serve as a blueprint for the analysis of traits and conditions linking crop yield and senescence.
Y1  - 2013
U6  - https://doi.org/10.1104/pp.113.217380
SN  - 0032-0889
VL  - 162
IS  - 3
SP  - 1290
EP  - 1310
PB  - American Society of Plant Physiologists
CY  - Rockville
ER  -