TY - JOUR A1 - Eckert, Silvia A1 - Herden, Jasmin A1 - Stift, Marc A1 - Joshi, Jasmin Radha A1 - van Kleunen, Mark T1 - Manipulation of cytosine methylation does not remove latitudinal clines in two invasive goldenrod species in Central Europe JF - Molecular ecology N2 - Invasive species frequently differentiate phenotypically in novel environments within a few generations, often even with limited genetic variation. For the invasive plants Solidago canadensis and S. gigantea, we tested whether such differentiation might have occurred through heritable epigenetic changes in cytosine methylation. In a 2-year common-garden experiment, we grew plants from seeds collected along a latitudinal gradient in their non-native Central European range to test for trait differentiation and whether differentiation disappeared when seeds were treated with the demethylation agent zebularine. Microsatellite markers revealed no population structure along the latitudinal gradient in S. canadensis, but three genetic clusters in S. gigantea. Solidago canadensis showed latitudinal clines in flowering phenology and growth. In S. gigantea, the number of clonal offspring decreased with latitude. Although zebularine had a significant effect on early growth, probably through effects on cytosine methylation, latitudinal clines remained (or even got stronger) in plants raised from seeds treated with zebularine. Thus, our experiment provides no evidence that epigenetic mechanisms by selective cytosine methylation contribute to the observed phenotypic differentiation in invasive goldenrods in Central Europe. KW - common‐garden experiment KW - epigenetic variation KW - microsatellites KW - Solidago canadensis KW - Solidago gigantea KW - zebularine Y1 - 2020 U6 - https://doi.org/10.1111/mec.15722 SN - 0962-1083 SN - 1365-294X VL - 30 IS - 1 SP - 222 EP - 236 PB - Wiley CY - Hoboken ER - TY - GEN A1 - Eckert, Silvia A1 - Herden, Jasmin A1 - Stift, Marc A1 - Joshi, Jasmin Radha A1 - van Kleunen, Mark T1 - Manipulation of cytosine methylation does not remove latitudinal clines in two invasive goldenrod species in Central Europe T2 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe N2 - Invasive species frequently differentiate phenotypically in novel environments within a few generations, often even with limited genetic variation. For the invasive plants Solidago canadensis and S. gigantea, we tested whether such differentiation might have occurred through heritable epigenetic changes in cytosine methylation. In a 2-year common-garden experiment, we grew plants from seeds collected along a latitudinal gradient in their non-native Central European range to test for trait differentiation and whether differentiation disappeared when seeds were treated with the demethylation agent zebularine. Microsatellite markers revealed no population structure along the latitudinal gradient in S. canadensis, but three genetic clusters in S. gigantea. Solidago canadensis showed latitudinal clines in flowering phenology and growth. In S. gigantea, the number of clonal offspring decreased with latitude. Although zebularine had a significant effect on early growth, probably through effects on cytosine methylation, latitudinal clines remained (or even got stronger) in plants raised from seeds treated with zebularine. Thus, our experiment provides no evidence that epigenetic mechanisms by selective cytosine methylation contribute to the observed phenotypic differentiation in invasive goldenrods in Central Europe. T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 1378 KW - common‐garden experiment KW - epigenetic variation KW - microsatellites KW - Solidago canadensis KW - Solidago gigantea KW - zebularine Y1 - 2020 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-569528 SN - 1866-8372 IS - 1 ER - TY - JOUR A1 - Herden, Jasmin A1 - Eckert, Silvia A1 - Stift, Marc A1 - Joshi, Jasmin Radha A1 - van Kleunen, Mark T1 - No evidence for local adaptation and an epigenetic underpinning in native and non-native ruderal plant species in Germany JF - Ecology and evolution N2 - Many invasive species have rapidly adapted to different environments in their new ranges. This is surprising, as colonization is usually associated with reduced genetic variation. Heritable phenotypic variation with an epigenetic basis may explain this paradox. Here, we assessed the contribution of DNA methylation to local adaptation in native and naturalized non-native ruderal plant species in Germany. We reciprocally transplanted offspring from natural populations of seven native and five non-native plant species between the Konstanz region in the south and the Potsdam region in the north of Germany. Before the transplant, half of the seeds were treated with the demethylation agent zebularine. We recorded survival, flowering probability, and biomass production as fitness estimates. Contrary to our expectations, we found little evidence for local adaptation, both among the native and among the non-native plant species. Zebularine treatment had mostly negative effects on overall plant performance, regardless of whether plants were local or not, and regardless of whether they were native or non-native. Synthesis. We conclude that local adaptation, at least at the scale of our study, plays no major role in the success of non-native and native ruderal plants. Consequently, we found no evidence yet for an epigenetic basis of local adaptation. KW - biological invasions KW - epigenetics KW - local adaptation KW - reciprocal transplant experiment KW - ruderal plant species KW - zebularine Y1 - 2019 U6 - https://doi.org/10.1002/ece3.5325 SN - 2045-7758 VL - 9 IS - 17 SP - 9412 EP - 9426 PB - Wiley CY - Hoboken ER - TY - JOUR A1 - Eckert, Silvia A1 - Herden, Jasmin A1 - Stift, Marc A1 - Durka, Walter A1 - Kleunen, Mark van A1 - Joshi, Jasmin Radha T1 - Traces of genetic but not epigenetic adaptation in the invasive goldenrod Solidago canadensis despite the absence of population structure JF - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe N2 - Biological invasions may result from multiple introductions, which might compensate for reduced gene pools caused by bottleneck events, but could also dilute adaptive processes. A previous common-garden experiment showed heritable latitudinal clines in fitness-related traits in the invasive goldenrod Solidago canadensis in Central Europe. These latitudinal clines remained stable even in plants chemically treated with zebularine to reduce epigenetic variation. However, despite the heritability of traits investigated, genetic isolation-by-distance was non-significant. Utilizing the same specimens, we applied a molecular analysis of (epi)genetic differentiation with standard and methylation-sensitive (MSAP) AFLPs. We tested whether this variation was spatially structured among populations and whether zebularine had altered epigenetic variation. Additionally, we used genome scans to mine for putative outlier loci susceptible to selection processes in the invaded range. Despite the absence of isolation-by-distance, we found spatial genetic neighborhoods among populations and two AFLP clusters differentiating northern and southern Solidago populations. Genetic and epigenetic diversity were significantly correlated, but not linked to phenotypic variation. Hence, no spatial epigenetic patterns were detected along the latitudinal gradient sampled. Applying genome-scan approaches (BAYESCAN, BAYESCENV, RDA, and LFMM), we found 51 genetic and epigenetic loci putatively responding to selection. One of these genetic loci was significantly more frequent in populations at the northern range. Also, one epigenetic locus was more frequent in populations in the southern range, but this pattern was lost under zebularine treatment. Our results point to some genetic, but not epigenetic adaptation processes along a large-scale latitudinal gradient of S. canadensis in its invasive range. T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 1271 KW - AFLP KW - MSAP KW - cytosine methylation KW - spatial autocorrelation KW - genome scan Y1 - 2022 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-566758 SN - 1866-8372 SP - 1 EP - 17 PB - Universitätsverlag Potsdam CY - Potsdam ER - TY - JOUR A1 - Guerrero-Ramirez, Nathaly Rokssana A1 - Craven, Dylan A1 - Reich, Peter B. A1 - Ewel, John J. A1 - Isbell, Forest A1 - Koricheva, Julia A1 - Parrotta, John A. A1 - Auge, Harald A1 - Erickson, Heather E. A1 - Forrester, David I. A1 - Hector, Andy A1 - Joshi, Jasmin Radha A1 - Montagnini, Florencia A1 - Palmborg, Cecilia A1 - Piotto, Daniel A1 - Potvin, Catherine A1 - Roscher, Christiane A1 - van Ruijven, Jasper A1 - Tilman, David A1 - Wilsey, Brian A1 - Eisenhauer, Nico T1 - Diversity-dependent temporal divergence of ecosystem functioning in experimental ecosystems JF - Nature ecology & evolution N2 - The effects of biodiversity on ecosystem functioning generally increase over time, but the underlying processes remain unclear. Using 26 long-term grassland and forest experimental ecosystems, we demonstrate that biodiversity-ecosystem functioning relationships strengthen mainly by greater increases in functioning in high-diversity communities in grasslands and forests. In grasslands, biodiversity effects also strengthen due to decreases in functioning in low-diversity communities. Contrasting trends across grasslands are associated with differences in soil characteristics. Y1 - 2017 U6 - https://doi.org/10.1038/s41559-017-0325-1 SN - 2397-334X VL - 1 IS - 11 SP - 1639 EP - 1642 PB - Nature Publ. Group CY - London ER - TY - JOUR A1 - Eugenia Tietze, Hedwig Selma A1 - Joshi, Jasmin Radha A1 - Pugnaire, Francisco Ignacio A1 - Dechoum, Michele de Sa T1 - Seed germination and seedling establishment of an invasive tropical tree species under different climate change scenarios JF - Austral ecology N2 - Increasing air temperature and atmospheric CO2 levels may affect the distribution of invasive species. Whereas there is wide knowledge on the effect of global change on temperate species, responses of tropical invasive species to these two global change drivers are largely unknown. We conducted a greenhouse experiment on Terminalia catappa L. (Combretaceae), an invasive tree species on Brazilian coastal areas, to evaluate the effects of increased air temperature and CO2 concentration on seed germination and seedling growth on the island of Santa Catarina (Florianopolis, Brazil). Seeds of the invasive tree were subjected to two temperature levels (ambient and +1.6 degrees C) and two CO2 levels (ambient and 650 ppmv) with a factorial design. Increased temperature enhanced germination rate and shortened germination time of T. catappa seeds. It also increased plant height, number of leaves and above-ground biomass. By contrast, increased atmospheric CO2 concentration had no significant effects, and the interaction between temperature and CO2 concentration did not affect any of the measured traits. Terminalia catappa adapts to a relatively broad range of environmental conditions, being able to tolerate cooler temperatures in its invasive range. As T. catappa is native to tropical areas, global warming might favour its establishment along the coast of subtropical South America, while increased CO2 levels seem not to have significant effects on seed germination or seedling growth. KW - CO2 concentration KW - coastal dunes KW - establishment KW - invasive plant KW - plant invasion KW - temperature KW - Terminalia catappa Y1 - 2019 U6 - https://doi.org/10.1111/aec.12809 SN - 1442-9985 SN - 1442-9993 VL - 44 IS - 8 SP - 1351 EP - 1358 PB - Wiley CY - Hoboken ER - TY - JOUR A1 - Schwarzer, Christian A1 - Joshi, Jasmin Radha T1 - Ecotypic differentiation, hybridization and clonality facilitate the persistence of a cold-adapted sedge in European bogs JF - Biological journal of the Linnean Society : a journal of evolution N2 - Recent research has shown that many cold-adapted species survived the last glacial maximum (LGM) in northern refugia. Whether this evolutionary history has had consequences for their genetic diversity and adaptive potential remains unknown. We sampled 14 populations of Carex limosa, a sedge specialized to bog ecosystems, along a latitudinal gradient from its Scandinavian core to the southern lowland range-margin in Germany. Using microsatellite and experimental common-garden data, we evaluated the impacts of global climate change along this gradient and assessed the conservation status of the southern marginal populations. Microsatellite data revealed two highly distinct genetic groups and hybrid individuals. In our common-garden experiment, the two groups showed divergent responses to increased nitrogen/phosphorus (N/P) availability, suggesting ecotypic differentiation. Each group formed genetically uniform populations at both northern and southern sampling areas. Mixed populations occurred throughout our sampling area, an area that was entirely glaciated during the LGM. The fragmented distribution implies allopatric divergence at geographically separated refugia that putatively differed in N/P availability. Molecular data and an observed low hybrid fecundity indicate the importance of clonal reproduction for hybrid populations. At the southern range-margin, however, all populations showed effects of clonality, lowered fecundity and low competitiveness, suggesting abiotic and biotic constraints to population persistence. KW - biogeography KW - bog/mire plants KW - Carex limosa KW - climate change KW - glacial divergence KW - global change KW - leading/trailing edge KW - population differentiation KW - sexual/asexual reproduction Y1 - 2019 U6 - https://doi.org/10.1093/biolinnean/blz141 SN - 0024-4066 SN - 1095-8312 VL - 128 IS - 4 SP - 909 EP - 925 PB - Oxford Univ. Press CY - Oxford ER - TY - JOUR A1 - Shi, Jun A1 - Joshi, Jasmin Radha A1 - Tielboerger, Katja A1 - Verhoeven, Koen J. F. A1 - Macel, Mirka T1 - Costs and benefits of admixture between foreign genotypes and local populations in the field JF - Ecology and evolution N2 - Admixture is the hybridization between populations within one species. It can increase plant fitness and population viability by alleviating inbreeding depression and increasing genetic diversity. However, populations are often adapted to their local environments and admixture with distant populations could break down local adaptation by diluting the locally adapted genomes. Thus, admixed genotypes might be selected against and be outcompeted by locally adapted genotypes in the local environments. To investigate the costs and benefits of admixture, we compared the performance of admixed and within-population F1 and F2 generations of the European plant Lythrum salicaria in a reciprocal transplant experiment at three European field sites over a 2-year period. Despite strong differences between site and plant populations for most of the measured traits, including herbivory, we found limited evidence for local adaptation. The effects of admixture depended on experimental site and plant population, and were positive for some traits. Plant growth and fruit production of some populations increased in admixed offspring and this was strongest with larger parental distances. These effects were only detected in two of our three sites. Our results show that, in the absence of local adaptation, admixture may boost plant performance, and that this is particularly apparent in stressful environments. We suggest that admixture between foreign and local genotypes can potentially be considered in nature conservation to restore populations and/or increase population viability, especially in small inbred or maladapted populations. KW - heterosis KW - inbreeding depression KW - local adaptation KW - Lythrum salicaria KW - outbreeding depression Y1 - 2018 U6 - https://doi.org/10.1002/ece3.3946 SN - 2045-7758 VL - 8 IS - 7 SP - 3675 EP - 3684 PB - Wiley CY - Hoboken ER - TY - JOUR A1 - Fischer, Markus A1 - Pfisterer, A. A1 - Joshi, Jasmin Radha A1 - Schmid, Bernhard T1 - Rapid decay of diversity-productivity relationships after invasion of experimental plant communities Y1 - 2004 ER - TY - JOUR A1 - Estendorfer, Jennifer A1 - Stempfhuber, Barbara A1 - Haury, Paula A1 - Vestergaard, Gisle A1 - Rillig, Matthias C. A1 - Joshi, Jasmin Radha A1 - Schröder, Peter A1 - Schloter, Michael T1 - The Influence of Land Use Intensity on the Plant-Associated Microbiome of Dactylis glomerata L. JF - Frontiers in plant science N2 - In this study, we investigated the impact of different land use intensities (LUI) on the root-associated microbiome of Dactylis glomerata (orchardgrass). For this purpose, eight sampling sites with different land use intensity levels but comparable soil properties were selected in the southwest of Germany. Experimental plots covered land use levels from natural grassland up to intensively managed meadows. We used 16S rRNA gene based barcoding to assess the plant-associated community structure in the endosphere, rhizosphere and bulk soil of D. glomerata. Samples were taken at the reproductive stage of the plant in early summer. Our data indicated that roots harbor a distinct bacterial community, which clearly differed from the microbiome of the rhizosphere and bulk soil. Our results revealed Pseudomonadaceae, Enterobacteriaceae and Comamonadaceae as the most abundant endophytes independently of land use intensity. Rhizosphere and bulk soil were dominated also by Proteobacteria, but the most abundant families differed from those obtained from root samples. In the soil, the effect of land use intensity was more pronounced compared to root endophytes leading to a clearly distinct pattern of bacterial communities under different LUI from rhizosphere and bulk soil vs. endophytes. Overall, a change of community structure on the plant-soil interface was observed, as the number of shared OTUs between all three compartments investigated increased with decreasing land use intensity. Thus, our findings suggest a stronger interaction of the plant with its surrounding soil under low land use intensity. Furthermore, the amount and quality of available nitrogen was identified as a major driver for shifts in the microbiome structure in all compartments. KW - Dactylis glomerata KW - land use change KW - endophytes KW - rhizosphere KW - soil microbiome KW - biodiversity Y1 - 2017 U6 - https://doi.org/10.3389/fpls.2017.00930 SN - 1664-462X VL - 8 PB - Frontiers Research Foundation CY - Lausanne ER -