TY  - JOUR
A1  - Vierheller, Janine
ED  - Lambrecht, Anna-Lena
ED  - Margaria, Tiziana
T1  - Exploratory Data Analysis
JF  - Process Design for Natural Scientists: an agile model-driven approach
N2  - In bioinformatics the term exploratory data analysis refers to different methods to get an overview of large biological data sets. Hence, it helps to create a framework for further analysis and hypothesis testing. The workflow facilitates this first important step of the data analysis created by high-throughput technologies. The results are different plots showing the structure of the measurements. The goal of the workflow is the automatization of the exploratory data analysis, but also the flexibility should be guaranteed. The basic tool is the free software R.
Y1  - 2014
SN  - 978-3-662-45005-5
SN  - 1865-0929
IS  - 500
SP  - 110
EP  - 126
PB  - Axel Springer Verlag
CY  - Berlin
ER  - 
TY  - JOUR
A1  - Bordag, Natalie
A1  - Klie, Sebastian
A1  - Jürchott, Kathrin
A1  - Vierheller, Janine
A1  - Schiewe, Hajo
A1  - Albrecht, Valerie
A1  - Tonn, Jörg-Christian
A1  - Schwartz, Christoph
A1  - Schichor, Christian
A1  - Selbig, Joachim
T1  - Glucocorticoid (dexamethasone)-induced metabolome changes in healthy males suggest prediction of response and side effects
JF  - Scientific reports
N2  - Glucocorticoids are indispensable anti-inflammatory and decongestant drugs with high prevalence of use at (similar to)0.9% of the adult population. Better holistic insights into glucocorticoid-induced changes are crucial for effective use as concurrent medication and management of adverse effects. The profiles of 214 metabolites from plasma of 20 male healthy volunteers were recorded prior to and after ingestion of a single dose of 4 mg dexamethasone (+20 mg pantoprazole). Samples were drawn at three predefined time points per day: seven untreated (day 1 midday - day 3 midday) and four treated (day 3 evening - day 4 evening) per volunteer. Statistical analysis revealed tremendous impact of dexamethasone on the metabolome with 150 of 214 metabolites being significantly deregulated on at least one time point after treatment (ANOVA, Benjamini-Hochberg corrected, q < 0.05). Inter-person variability was high and remained uninfluenced by treatment. The clearly visible circadian rhythm prior to treatment was almost completely suppressed and deregulated by dexamethasone. The results draw a holistic picture of the severe metabolic deregulation induced by single-dose, short-term glucocorticoid application. The observed metabolic changes suggest a potential for early detection of severe side effects, raising hope for personalized early countermeasures increasing quality of life and reducing health care costs.
Y1  - 2015
U6  - https://doi.org/10.1038/srep15954
SN  - 2045-2322
VL  - 5
PB  - Nature Publ. Group
CY  - London
ER  -