TY - JOUR A1 - Schulz, Irene A1 - Erle, Alexander A1 - Gräf, Ralph A1 - Krueger, Anne A1 - Lohmeier, Heiner A1 - Putzler, Sascha A1 - Samereier, Matthias A1 - Weidenthaler, Sebastian T1 - Identification and cell cycle-dependent localization of nine novel, genuine centrosomal components in Dictyostelium discoideum N2 - The centrosome is the main microtubule-organizing center and constitutes the largest protein complex in a eukaryotic cell. The Dictyostelium centrosome is an established model for acentriolar centrosomes and it consists of a layered core structure Surrounded by a so-called corona, which harbors microtubule nucleation complexes. We have identified 34 new centrosomal candidate proteins through mass spectrometrical analysis of the proteome of isolated Dictyostelium centrosomes. Here we present a characterization of 12 centrosomal candidate proteins all featuring coiled coil regions and low expression levels, which are the most common attributes of centrosomal proteins. We used GFP fusion proteins to localize the candidate proteins in whole cells and on microtubule-free, isolated centrosomes. Thus we were able to identify nine new genuine centrosomal proteins including a putative orthologue of Cep192, an interaction partner of polo-like kinase 4 in human centriole biogenesis. In this respect, centrosomal localization of the only polo-like kinase in Dictyostelium, Pik, is also shown in this work. Using confocal deconvolution microscopy, four components, CP39, CP55, CP75, and CP91 could be clearly assigned to the so far almost uncharacterized centrosomal core structure, while CP148 and Cep192 localized to a zone between that of corona marker and core proteins. Finally, CP103 and CP248 were constituents of the corona. In contrast, NE81 was localized at the nuclear envelope and three others, an orthologue of the spindle checkpoint component Mad1, the novel Cenp68, and the centrosomal CP248 were observed at the centromeres, which are clustered and linked to the centrosome throughout the entire cell cycle. Cell Motil. Cytoskeleton 66: 915-928, 2009. Y1 - 2009 UR - http://www3.interscience.wiley.com/cgi-bin/jhome/36113/ U6 - https://doi.org/10.1002/Cm.20384 SN - 0886-1544 ER - TY - JOUR A1 - Schulz, Irene A1 - Baumann, Otto A1 - Samereier, Matthias A1 - Zoglmeier, Christine A1 - Gräf, Ralph T1 - Dictyostelium Sun1 is a dynamic membrane protein of both nuclear membranes and required for centrosomal association with clustered centromeres N2 - Centrosomal attachment to nuclei is crucial for proper mitosis and nuclear positioning in various organisms, and generally involves Sun-family proteins located at the inner nuclear envelope. There is still no common scheme for the outer nuclear membrane proteins interacting with Sun I in centrosome/nucleus attachment. Here we propose a model in which Sun1 mediates a physical link between centrosomes and clustered centromeres through both nuclear membranes in Dictyostelium. For the first time we provide a detailed microscopic analysis of the centrosomal and nuclear envelope localization of endogenous Dictyostelium Sun1 during interphase and mitosis. By immunogold electron microscopy we show that Sun1 is a resident of both nuclear membranes. Disruption of Sun1 function by overexpression of full-length GFP-Sun1 or a GFP-Sun-domain deletion construct revealed not only the established function in centrosome/nucleus attachment and maintenance of ploidy, but also a requirement of Sun1 for the association of the centromere cluster with the centrosome. Live-cell imaging visualized the occurrence of mitotic defects, and demonstrated the requirement of microtubules for dynamic distance changes between centrosomes and nuclei. FRAP analysis revealed at least two populations of Sun1, with an immobile fraction associated with the centrosome, and a mobile fraction in the nuclear envelope. Y1 - 2009 UR - http://www.sciencedirect.com/science/journal/01719335 U6 - https://doi.org/10.1016/j.ejcb.2009.06.003 SN - 0171-9335 ER - TY - JOUR A1 - Schulz, Irene A1 - Erle, Alexander A1 - Gräf, Ralph A1 - Krueger, Anne A1 - Putzler, Sascha A1 - Samereier, Matthias A1 - Weidenthaler, Sebastian T1 - Cell cycle-dependent localization of novel centrosomal and centromeric proteins in Dictyostelium Y1 - 2009 UR - http://www.sciencedirect.com/science/journal/01719335 U6 - https://doi.org/10.1016/S0171-9335(09)00023-5 SN - 0171-9335 ER - TY - BOOK A1 - Rana, Kaushik A1 - Mohapatra, Durga Prasad A1 - Sidorova, Julia A1 - Lundberg, Lars A1 - Sköld, Lars A1 - Lopes Grim, Luís Fernando A1 - Sampaio Gradvohl, André Leon A1 - Cremerius, Jonas A1 - Siegert, Simon A1 - Weltzien, Anton von A1 - Baldi, Annika A1 - Klessascheck, Finn A1 - Kalancha, Svitlana A1 - Lichtenstein, Tom A1 - Shaabani, Nuhad A1 - Meinel, Christoph A1 - Friedrich, Tobias A1 - Lenzner, Pascal A1 - Schumann, David A1 - Wiese, Ingmar A1 - Sarna, Nicole A1 - Wiese, Lena A1 - Tashkandi, Araek Sami A1 - van der Walt, Estée A1 - Eloff, Jan H. P. A1 - Schmidt, Christopher A1 - Hügle, Johannes A1 - Horschig, Siegfried A1 - Uflacker, Matthias A1 - Najafi, Pejman A1 - Sapegin, Andrey A1 - Cheng, Feng A1 - Stojanovic, Dragan A1 - Stojnev Ilić, Aleksandra A1 - Djordjevic, Igor A1 - Stojanovic, Natalija A1 - Predic, Bratislav A1 - González-Jiménez, Mario A1 - de Lara, Juan A1 - Mischkewitz, Sven A1 - Kainz, Bernhard A1 - van Hoorn, André A1 - Ferme, Vincenzo A1 - Schulz, Henning A1 - Knigge, Marlene A1 - Hecht, Sonja A1 - Prifti, Loina A1 - Krcmar, Helmut A1 - Fabian, Benjamin A1 - Ermakova, Tatiana A1 - Kelkel, Stefan A1 - Baumann, Annika A1 - Morgenstern, Laura A1 - Plauth, Max A1 - Eberhard, Felix A1 - Wolff, Felix A1 - Polze, Andreas A1 - Cech, Tim A1 - Danz, Noel A1 - Noack, Nele Sina A1 - Pirl, Lukas A1 - Beilharz, Jossekin Jakob A1 - De Oliveira, Roberto C. L. A1 - Soares, Fábio Mendes A1 - Juiz, Carlos A1 - Bermejo, Belen A1 - Mühle, Alexander A1 - Grüner, Andreas A1 - Saxena, Vageesh A1 - Gayvoronskaya, Tatiana A1 - Weyand, Christopher A1 - Krause, Mirko A1 - Frank, Markus A1 - Bischoff, Sebastian A1 - Behrens, Freya A1 - Rückin, Julius A1 - Ziegler, Adrian A1 - Vogel, Thomas A1 - Tran, Chinh A1 - Moser, Irene A1 - Grunske, Lars A1 - Szárnyas, Gábor A1 - Marton, József A1 - Maginecz, János A1 - Varró, Dániel A1 - Antal, János Benjamin ED - Meinel, Christoph ED - Polze, Andreas ED - Beins, Karsten ED - Strotmann, Rolf ED - Seibold, Ulrich ED - Rödszus, Kurt ED - Müller, Jürgen T1 - HPI Future SOC Lab – Proceedings 2018 N2 - The “HPI Future SOC Lab” is a cooperation of the Hasso Plattner Institute (HPI) and industry partners. Its mission is to enable and promote exchange and interaction between the research community and the industry partners. The HPI Future SOC Lab provides researchers with free of charge access to a complete infrastructure of state of the art hard and software. This infrastructure includes components, which might be too expensive for an ordinary research environment, such as servers with up to 64 cores and 2 TB main memory. The offerings address researchers particularly from but not limited to the areas of computer science and business information systems. Main areas of research include cloud computing, parallelization, and In-Memory technologies. This technical report presents results of research projects executed in 2018. Selected projects have presented their results on April 17th and November 14th 2017 at the Future SOC Lab Day events. N2 - Das Future SOC Lab am HPI ist eine Kooperation des Hasso-Plattner-Instituts mit verschiedenen Industriepartnern. Seine Aufgabe ist die Ermöglichung und Förderung des Austausches zwischen Forschungsgemeinschaft und Industrie. Am Lab wird interessierten Wissenschaftler:innen eine Infrastruktur von neuester Hard- und Software kostenfrei für Forschungszwecke zur Verfügung gestellt. Dazu zählen Systeme, die im normalen Hochschulbereich in der Regel nicht zu finanzieren wären, bspw. Server mit bis zu 64 Cores und 2 TB Hauptspeicher. Diese Angebote richten sich insbesondere an Wissenschaftler:innen in den Gebieten Informatik und Wirtschaftsinformatik. Einige der Schwerpunkte sind Cloud Computing, Parallelisierung und In-Memory Technologien. In diesem Technischen Bericht werden die Ergebnisse der Forschungsprojekte des Jahres 2018 vorgestellt. Ausgewählte Projekte stellten ihre Ergebnisse am 17. April und 14. November 2018 im Rahmen des Future SOC Lab Tags vor. T3 - Technische Berichte des Hasso-Plattner-Instituts für Digital Engineering an der Universität Potsdam - 151 KW - Future SOC Lab KW - research projects KW - multicore architectures KW - in-memory technology KW - cloud computing KW - machine learning KW - artifical intelligence KW - Future SOC Lab KW - Forschungsprojekte KW - Multicore Architekturen KW - In-Memory Technologie KW - Cloud Computing KW - maschinelles Lernen KW - künstliche Intelligenz Y1 - 2022 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-563712 SN - 978-3-86956-547-7 SN - 1613-5652 SN - 2191-1665 IS - 151 PB - Universitätsverlag Potsdam CY - Potsdam ER -