TY  - JOUR
A1  - Barchewitz, Tino
A1  - Guljamow, Arthur
A1  - Meißner, Sven
A1  - Timm, Stefan
A1  - Henneberg, Manja
A1  - Baumann, Otto
A1  - Hagemann, Martin
A1  - Dittmann, Elke
T1  - Non-canonical localization of RubisCO under high-light conditions in the toxic cyanobacterium Microcystis aeruginosa PCC7806
JF  - Environmental microbiology
N2  - The frequent production of the hepatotoxin microcystin (MC) and its impact on the lifestyle of bloom-forming cyanobacteria are poorly understood. Here, we report that MC interferes with the assembly and the subcellular localization of RubisCO, in Microcystis aeruginosa PCC7806. Immunofluorescence, electron microscopic and cellular fractionation studies revealed a pronounced heterogeneity in the subcellular localization of RubisCO. At high cell density, RubisCO particles are largely separate from carboxysomes in M. aeruginosa and relocate to the cytoplasmic membrane under high-light conditions. We hypothesize that the binding of MC to RubisCO promotes its membrane association and enables an extreme versatility of the enzyme. Steady-state levels of the RubisCO CO2 fixation product 3-phosphoglycerate are significantly higher in the MC-producing wild type. We also detected noticeable amounts of the RubisCO oxygenase reaction product secreted into the medium that may support the mutual interaction of M. aeruginosa with its heterotrophic microbial community.
Y1  - 2019
U6  - https://doi.org/10.1111/1462-2920.14837
SN  - 1462-2912
SN  - 1462-2920
VL  - 21
IS  - 12
SP  - 4836
EP  - 4851
PB  - Wiley
CY  - Hoboken
ER  -