TY  - GEN
A1  - Hardy, John G.
A1  - Torres-Rendon, Jose Guillermo
A1  - Leal-Egaña, Aldo
A1  - Walther, Andreas
A1  - Schlaad, Helmut
A1  - Cölfen, Helmut
A1  - Scheibel, Thomas R.
T1  - Biomineralization of engineered spider silk protein-based composite materials for bone tissue engineering
N2  - Materials based on biodegradable polyesters, such as poly(butylene terephthalate) (PBT) or poly(butylene terephthalate-co-poly(alkylene glycol) terephthalate) (PBTAT), have potential application as pro-regenerative scaffolds for bone tissue engineering. Herein, the preparation of films composed of PBT or PBTAT and an engineered spider silk protein, (eADF4(C16)), that displays multiple carboxylic acid moieties capable of binding calcium ions and facilitating their biomineralization with calcium carbonate or calcium phosphate is reported. Human mesenchymal stem cells cultured on films mineralized with calcium phosphate show enhanced levels of alkaline phosphatase activity suggesting that such composites have potential use for bone tissue engineering.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 359 
KW  - spider silk
KW  - recombinant protein
KW  - biodegradable polymers
KW  - biomaterials
KW  - biomineralization
KW  - bone tissue engineering
Y1  - 2017
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-400519
ER  - 
TY  - JOUR
A1  - Hardy, John G.
A1  - Torres-Rendon, Jose Guillermo
A1  - Leal-Egana, Aldo
A1  - Walther, Andreas
A1  - Schlaad, Helmut
A1  - Coelfen, Helmut
A1  - Scheibel, Thomas R.
T1  - Biomineralization of Engineered Spider Silk Protein-Based Composite Materials for Bone Tissue Engineering
JF  - Materials
N2  - Materials based on biodegradable polyesters, such as poly(butylene terephthalate) (PBT) or poly(butylene terephthalate-co-poly(alkylene glycol) terephthalate) (PBTAT), have potential application as pro-regenerative scaffolds for bone tissue engineering. Herein, the preparation of films composed of PBT or PBTAT and an engineered spider silk protein, (eADF4(C16)), that displays multiple carboxylic acid moieties capable of binding calcium ions and facilitating their biomineralization with calcium carbonate or calcium phosphate is reported. Human mesenchymal stem cells cultured on films mineralized with calcium phosphate show enhanced levels of alkaline phosphatase activity suggesting that such composites have potential use for bone tissue engineering.
KW  - spider silk
KW  - recombinant protein
KW  - biodegradable polymers
KW  - biomaterials
KW  - biomineralization
KW  - bone tissue engineering
Y1  - 2016
U6  - https://doi.org/10.3390/ma9070560
SN  - 1996-1944
VL  - 9
SP  - 93
EP  - 108
PB  - MDPI
CY  - Basel
ER  - 
TY  - JOUR
A1  - Hardy, John G.
A1  - Bertin, Annabelle
A1  - Torres-Rendon, Jose Guillermo
A1  - Leal-Egana, Aldo
A1  - Humenik, Martin
A1  - Bauer, Felix
A1  - Walther, Andreas
A1  - Cölfen, Helmut
A1  - Schlaad, Helmut
A1  - Scheibel, Thomas R.
T1  - Facile photochemical modification of silk protein-based biomaterials
JF  - Macromolecular bioscience
N2  - Silk protein-based materials show promise for application as biomaterials for tissue engineering. The simple and rapid photochemical modification of silk protein-based materials composed of either Bombyx mori silkworm silk or engineered spider silk proteins (eADF4(C16)) is reported. Radicals formed on the silk-based materials initiate the polymerization of monomers (acrylic acid, methacrylic acid, or allylamine) which functionalize the surface of the silk materials with poly(acrylic acid) (PAA), poly(methacrylic acid) (PMAA), or poly(allylamine) (PAAm). To demonstrate potential applications of this type of modification, the polymer-modified silks are mineralized. The PAA- and PMAA-functionalized silks are mineralized with calcium carbonate, whereas the PAAm-functionalized silks are mineralized with silica, both of which provide a coating on the materials that may be useful for bone tissue engineering, which will be the subject of future investigations.
KW  - biomaterials
KW  - chemical modification
KW  - photochemistry
KW  - silkworm silk
KW  - spider silk
Y1  - 2018
U6  - https://doi.org/10.1002/mabi.201800216
SN  - 1616-5187
SN  - 1616-5195
VL  - 18
IS  - 11
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Fages, Antoine
A1  - Hanghoj, Kristian
A1  - Khan, Naveed
A1  - Gaunitz, Charleen
A1  - Seguin-Orlando, Andaine
A1  - Leonardi, Michela
A1  - Constantz, Christian McCrory
A1  - Gamba, Cristina
A1  - Al-Rasheid, Khaled A. S.
A1  - Albizuri, Silvia
A1  - Alfarhan, Ahmed H.
A1  - Allentoft, Morten
A1  - Alquraishi, Saleh
A1  - Anthony, David
A1  - Baimukhanov, Nurbol
A1  - Barrett, James H.
A1  - Bayarsaikhan, Jamsranjav
A1  - Benecke, Norbert
A1  - Bernaldez-Sanchez, Eloisa
A1  - Berrocal-Rangel, Luis
A1  - Biglari, Fereidoun
A1  - Boessenkool, Sanne
A1  - Boldgiv, Bazartseren
A1  - Brem, Gottfried
A1  - Brown, Dorcas
A1  - Burger, Joachim
A1  - Crubezy, Eric
A1  - Daugnora, Linas
A1  - Davoudi, Hossein
A1  - Damgaard, Peter de Barros
A1  - de Chorro y de Villa-Ceballos, Maria de los Angeles
A1  - Deschler-Erb, Sabine
A1  - Detry, Cleia
A1  - Dill, Nadine
A1  - Oom, Maria do Mar
A1  - Dohr, Anna
A1  - Ellingvag, Sturla
A1  - Erdenebaatar, Diimaajav
A1  - Fathi, Homa
A1  - Felkel, Sabine
A1  - Fernandez-Rodriguez, Carlos
A1  - Garcia-Vinas, Esteban
A1  - Germonpre, Mietje
A1  - Granado, Jose D.
A1  - Hallsson, Jon H.
A1  - Hemmer, Helmut
A1  - Hofreiter, Michael
A1  - Kasparov, Aleksei
A1  - Khasanov, Mutalib
A1  - Khazaeli, Roya
A1  - Kosintsev, Pavel
A1  - Kristiansen, Kristian
A1  - Kubatbek, Tabaldiev
A1  - Kuderna, Lukas
A1  - Kuznetsov, Pavel
A1  - Laleh, Haeedeh
A1  - Leonard, Jennifer A.
A1  - Lhuillier, Johanna
A1  - von Lettow-Vorbeck, Corina Liesau
A1  - Logvin, Andrey
A1  - Lougas, Lembi
A1  - Ludwig, Arne
A1  - Luis, Cristina
A1  - Arruda, Ana Margarida
A1  - Marques-Bonet, Tomas
A1  - Silva, Raquel Matoso
A1  - Merz, Victor
A1  - Mijiddorj, Enkhbayar
A1  - Miller, Bryan K.
A1  - Monchalov, Oleg
A1  - Mohaseb, Fatemeh A.
A1  - Morales, Arturo
A1  - Nieto-Espinet, Ariadna
A1  - Nistelberger, Heidi
A1  - Onar, Vedat
A1  - Palsdottir, Albina H.
A1  - Pitulko, Vladimir
A1  - Pitskhelauri, Konstantin
A1  - Pruvost, Melanie
A1  - Sikanjic, Petra Rajic
A1  - Papesa, Anita Rapan
A1  - Roslyakova, Natalia
A1  - Sardari, Alireza
A1  - Sauer, Eberhard
A1  - Schafberg, Renate
A1  - Scheu, Amelie
A1  - Schibler, Jorg
A1  - Schlumbaum, Angela
A1  - Serrand, Nathalie
A1  - Serres-Armero, Aitor
A1  - Shapiro, Beth
A1  - Seno, Shiva Sheikhi
A1  - Shevnina, Irina
A1  - Shidrang, Sonia
A1  - Southon, John
A1  - Star, Bastiaan
A1  - Sykes, Naomi
A1  - Taheri, Kamal
A1  - Taylor, William
A1  - Teegen, Wolf-Rudiger
A1  - Vukicevic, Tajana Trbojevic
A1  - Trixl, Simon
A1  - Tumen, Dashzeveg
A1  - Undrakhbold, Sainbileg
A1  - Usmanova, Emma
A1  - Vahdati, Ali
A1  - Valenzuela-Lamas, Silvia
A1  - Viegas, Catarina
A1  - Wallner, Barbara
A1  - Weinstock, Jaco
A1  - Zaibert, Victor
A1  - Clavel, Benoit
A1  - Lepetz, Sebastien
A1  - Mashkour, Marjan
A1  - Helgason, Agnar
A1  - Stefansson, Kari
A1  - Barrey, Eric
A1  - Willerslev, Eske
A1  - Outram, Alan K.
A1  - Librado, Pablo
A1  - Orlando, Ludovic
T1  - Tracking five millennia of horse management with extensive ancient genome time series
JF  - Cell
N2  - Horse domestication revolutionized warfare and accelerated travel, trade, and the geographic expansion of languages. Here, we present the largest DNA time series for a non-human organism to date, including genome-scale data from 149 ancient animals and 129 ancient genomes (>= 1-fold coverage), 87 of which are new. This extensive dataset allows us to assess the modem legacy of past equestrian civilisations. We find that two extinct horse lineages existed during early domestication, one at the far western (Iberia) and the other at the far eastern range (Siberia) of Eurasia. None of these contributed significantly to modern diversity. We show that the influence of Persian-related horse lineages increased following the Islamic conquests in Europe and Asia. Multiple alleles associated with elite-racing, including at the MSTN "speed gene," only rose in popularity within the last millennium. Finally, the development of modem breeding impacted genetic diversity more dramatically than the previous millennia of human management.
Y1  - 2019
U6  - https://doi.org/10.1016/j.cell.2019.03.049
SN  - 0092-8674
SN  - 1097-4172
VL  - 177
IS  - 6
SP  - 1419
EP  - 1435
PB  - Cell Press
CY  - Cambridge
ER  - 
TY  - JOUR
A1  - John, Helmut
A1  - Keßler, Christine
T1  - Interpretatorische Zugänge zu einem poetischen Text : Heiner Müller: Der glücklose Engel
Y1  - 1995
ER  - 
TY  - BOOK
A1  - Richter, Steffen
A1  - John, Helmut
T1  - Hermann Kasack 1896 - 1966 : eine Bibliographie
Y1  - 1996
PB  - Stadt- und Landesbibliothek
CY  - Potsdam
ER  - 
TY  - JOUR
A1  - Keßler, Christine
A1  - John, Helmut
T1  - Sprachskepsis in poetischen Texten
Y1  - 1997
ER  - 
TY  - JOUR
A1  - Moreno, Marcelo Spegiorin
A1  - Melnick, Daniel
A1  - Rosenau, M.
A1  - Bolte, John
A1  - Klotz, Jan
A1  - Echtler, Helmut Peter
A1  - Báez, Juan Carlos
A1  - Bataille, Klaus
A1  - Chen, J.
A1  - Bevis, M.
A1  - Hase, H.
A1  - Oncken, Onno
T1  - Heterogeneous plate locking in the South-Central Chile subduction zone  building up the next great earthquake
JF  - Earth & planetary science letters
N2  - We use Global Positioning System (GPS) velocities and kinematic Finite Element models (FE-models) to infer the state of locking between the converging Nazca and South America plates in South-Central Chile (36 degrees S -46 degrees S) and to evaluate its spatial and temporal variability. GPS velocities provide information on earthquake-cycle deformation over the last decade in areas affected by the megathrust events of 1960 (M-w = 9.5) and 2010 (M-w = 8.8). Our data confirm that a change in surface velocity patterns of these two seismotectonic segments can be related to their different stages in the seismic cycle: Accordingly, the northern (2010) segment was in a final stage of interseismic loading whereas the southern (1960) segment is still in a postseismic stage and undergoes a prolonged viscoelastic mantle relaxation. After correcting the signals for mantle relaxation, the residual GPS velocity pattern suggests that the plate interface accumulates slip deficit in a spatially and presumably temporally variable way towards the next great event. Though some similarity exist between locking and 1960 coseismic slip, extrapolating the current, decadal scale slip deficit accumulation towards the similar to 300-yr recurrence times of giant events here does neither yield the slip distribution nor the moment magnitude of the 1960 earthquake. This suggests that either the locking pattern is evolving in time (to reconcile a slip deficit distribution similar to the 1960 earthquake) or that some asperities are not persistent over multiple events. The accumulated moment deficit since 1960 suggests that highly locked patches in the 1960 segment are already capable of producing a M similar to 8 event if triggered to fail by stress transfer from the 2010 event.
KW  - GPS
KW  - Chile
KW  - Maule
KW  - locking degree
KW  - postseismic deformation
KW  - earthquake cycle
Y1  - 2011
U6  - https://doi.org/10.1016/j.epsl.2011.03.025
SN  - 0012-821X
VL  - 305
IS  - 3-4
SP  - 413
EP  - 424
PB  - Elsevier
CY  - Amsterdam
ER  -