TY  - JOUR
A1  - Baessler, Olivia Y.
A1  - Weiss, Julia
A1  - Wienkoop, Stefanie
A1  - Lehmann, Karola
A1  - Scheler, Christian
A1  - Doelle, Sabine
A1  - Schwarz, Dietmar
A1  - Franken, Philipp
A1  - George, Eckhard
A1  - Worm, Margitta
A1  - Weckwerth, Wolfram
T1  - Evidence for novel tomato seed allergens : IgE-reactive legumin and vicilin proteins identified by multidimensional protein fractionation-mass spectrometry and in silico epitope modeling
N2  - Tomato fruit and seed allergens were detected by IgE-immunoblotting using sera from 18 adult tomato-sensitized patients selected based on a positive history skin prick test (SPT) and specific Immunglobulin (Ig) E-levels. Isolated tomato seed total protein showed high SPT activity comparable or even higher than tomato fruit protein. For the molecular characterization of tomato seed allergens, a multidimensional protein fractionation strategy and LC-MS/MS was used. Two legumin- and vicilin-proteins were purified and showed strong IgE-reactivity in immunoblots. Individual patient sera exhibited varying IgE-sensitivity against the purified proteins. In silico structural modeling indicates high homology between epitopes of known walnut allergens and the detected IgE-crossreactive tomato proteins.
Y1  - 2009
UR  - http://pubs.acs.org/journal/jprobs
U6  - https://doi.org/10.1021/Pr800186d
SN  - 1535-3893
ER  -