TY  - JOUR
A1  - Bier, Frank Fabian
A1  - Ehrentreich-Förster, Eva
A1  - Scheller, Frieder W.
A1  - Makower, Alexander
A1  - Eremenko, A. V.
A1  - Wollenberger, Ursula
A1  - Bauer, Christian G.
A1  - Pfeiffer, Dorothea
A1  - Micheel, Burkhard
T1  - Ultrasensitive biosensors
Y1  - 1996
ER  - 
TY  - JOUR
A1  - Ghindilis, A. L.
A1  - Makower, Alexander
A1  - Bauer, Christian G.
A1  - Bier, Frank Fabian
A1  - Scheller, Frieder W.
T1  - Determination of p-aminophenol and catecholamines at picomolar concentrations based on recycling enzyme amplification
Y1  - 1995
ER  - 
TY  - JOUR
A1  - Scheller, Frieder W.
A1  - Makower, Alexander
A1  - Bier, Frank Fabian
A1  - Wollenberger, Ursula
A1  - Ghindilis, A. L.
A1  - Eremenko, A. V.
A1  - Pfeiffer, Dorothea
T1  - Enzymsensoren zur Bestimmung subnanomolarer Konzentrationen
Y1  - 1995
ER  - 
TY  - JOUR
A1  - Bier, Frank Fabian
A1  - Ehrentreich-Förster, Eva
A1  - Makower, Alexander
A1  - Scheller, Frieder W.
T1  - An enzymatic amplification cycle for high sensitive immunoassay
Y1  - 1996
ER  - 
TY  - JOUR
A1  - Song, Min Ik
A1  - Bier, Frank Fabian
A1  - Scheller, Frieder W.
T1  - A method to detect superoxide radicals using teflon membrane and superoxide dismutase
Y1  - 1995
ER  - 
TY  - JOUR
A1  - Scheller, Frieder W.
A1  - Bier, Frank Fabian
A1  - Pfeiffer, Dorothea
T1  - Biosensoren : Grundlagen und Anwendungen
Y1  - 1995
ER  - 
TY  - JOUR
A1  - Scheller, Frieder W.
A1  - Makower, Alexander
A1  - Ghindilis, A. L.
A1  - Bier, Frank Fabian
A1  - Ehrentreich-Förster, Eva
A1  - Wollenberger, Ursula
A1  - Bauer, Christian G.
A1  - Micheel, Burkhard
A1  - Pfeiffer, Dorothea
A1  - Szeponik, Jan
A1  - Michael, N.
A1  - Kaden, H.
T1  - Enzyme sensors for subnanomolar concentrations
Y1  - 1995
ER  - 
TY  - JOUR
A1  - Jin, Wen
A1  - Wollenberger, Ursula
A1  - Bier, Frank Fabian
A1  - Scheller, Frieder W.
T1  - Construction and characterization of multi-layer-enzyme electrode : covalent binding of quinoprotein glucose dehydrogenase onto gold electrodes
Y1  - 1995
ER  - 
TY  - GEN
A1  - Bier, Frank Fabian
A1  - Yin, Wen
A1  - Kleinjung, Frank
A1  - Scheller, Frieder W.
T1  - Molekulare Schichten zur Analyse biochemischer Bindungen und Umsatzreaktionen
Y1  - 1995
ER  - 
TY  - JOUR
A1  - Kleinjung, Frank
A1  - Bier, Frank Fabian
A1  - Scheller, Frieder W.
T1  - Messungen an Nukleinsäuren mittels evaneszenten Feldes
Y1  - 1995
ER  - 
TY  - JOUR
A1  - Bier, Frank Fabian
A1  - Scheller, Frieder W.
A1  - Klingbeil, Mandy
A1  - Oßwald, U.
T1  - Biosensoren und Teststreifen für die Umwelt- und Lebensmittelanalytik : eine Übersicht
Y1  - 1993
ER  - 
TY  - JOUR
A1  - Scheller, Frieder W.
A1  - Bier, Frank Fabian
A1  - Neumann, B.
T1  - Bioindikation in aquatischen Ökosystemen : Bioindikation in limnischen und küstennahen Ökosystemen ; Grundlagen, Verfahren und Methoden
Y1  - 1994
PB  - Fischer
CY  - Jena
ER  - 
TY  - JOUR
A1  - Dechtrirat, Decha
A1  - Gajovic-Eichelmann, Nenad
A1  - Wojcik, Felix
A1  - Hartmann, Laura
A1  - Bier, Frank Fabian
A1  - Scheller, Frieder W.
T1  - Electrochemical displacement sensor based on ferrocene boronic acid tracer and immobilized glycan for saccharide binding proteins and E. coli
JF  - Biosensors and bioelectronics : the principal international journal devoted to research, design development and application of biosensors and bioelectronics
N2  - Pathogens such as viruses and bacteria use their envelope proteins and their adhesin lectins to recognize the glycan residues presented on the cell surface of the target tissues. This principle of recognition is used in a new electrochemical displacement sensor for the protein concanavalin A (ConA). A gold electrode was first modified with a self-assembled monolayer of a thiolated mannose/OEG conjugate and a ferrocene boroxol derivative was pre-assembled as reporter molecule onto the mannose surface. The novel tracer molecule based on a 2-hydroxymethyl phenyl boronic acid derivative binds even at neutral pH to the saccharides which could expand the application towards biological samples (i.e., urine and feces). Upon the binding of ConA, the tracer was displaced and washed away from the sensor surface leading to a decrease in the electrochemical signal. Using square wave voltammetry (SWV), the concentration of ConA in the sample solution could be determined in the dynamic concentration range established from 38 nmol L-1 to 5.76 mu mol L-1 with a reproducible detection limit of 1 mu g mL(-1) (38 nmol L-1) based on the signal-to-noise ratio (S/N=3) with fast response of 15 min. The new reporter molecule showed a reduced non-specific displacement by BSA and ribonuclease A. The sensor was also successfully transferred to the first proof of principle for the detection of Escherichia coli exhibiting a detection limit of approximately 6 x 102 cells/mL Specificity of the displacement by target protein ConA and E. coli was demonstrated since the control proteins (i.e., BSA and RNaseA) and the control E. coli strain, which lack of type 1 fimbriae, were ineffective. (C) 2014 Elsevier B.V. All rights reserved.
KW  - Ferrocene benzoboroxol biosensor
KW  - Concanavalin A
KW  - Displacement
KW  - Escherichia coli
KW  - Ferrocene boronic acid
KW  - Self-assembled monolayer
Y1  - 2014
U6  - https://doi.org/10.1016/j.bios.2014.02.028
SN  - 0956-5663
SN  - 1873-4235
VL  - 58
SP  - 1
EP  - 8
PB  - Elsevier
CY  - Oxford
ER  - 
TY  - JOUR
A1  - Scheller, Frieder W.
A1  - Yarman, Aysu
A1  - Bachmann, Till
A1  - Hirsch, Thomas
A1  - Kubick, Stefan
A1  - Renneberg, Reinhard
A1  - Schumacher, Soeren
A1  - Wollenberger, Ursula
A1  - Teller, Carsten
A1  - Bier, Frank Fabian
ED  - Gu, MB
ED  - Kim, HS
T1  - Future of biosensors: a personal view
JF  - Advances in biochemical engineering, biotechnology
JF  - Advances in Biochemical Engineering-Biotechnology
N2  - Biosensors representing the technological counterpart of living senses have found routine application in amperometric enzyme electrodes for decentralized blood glucose measurement, interaction analysis by surface plasmon resonance in drug development, and to some extent DNA chips for expression analysis and enzyme polymorphisms. These technologies have already reached a highly advanced level and need minor improvement at most. The dream of the "100-dollar' personal genome may come true in the next few years provided that the technological hurdles of nanopore technology or of polymerase-based single molecule sequencing can be overcome. Tailor-made recognition elements for biosensors including membrane-bound enzymes and receptors will be prepared by cell-free protein synthesis. As alternatives for biological recognition elements, molecularly imprinted polymers (MIPs) have been created. They have the potential to substitute antibodies in biosensors and biochips for the measurement of low-molecular-weight substances, proteins, viruses, and living cells. They are more stable than proteins and can be produced in large amounts by chemical synthesis. Integration of nanomaterials, especially of graphene, could lead to new miniaturized biosensors with high sensitivity and ultrafast response. In the future individual therapy will include genetic profiling of isoenzymes and polymorphic forms of drug-metabolizing enzymes especially of the cytochrome P450 family. For defining the pharmacokinetics including the clearance of a given genotype enzyme electrodes will be a useful tool. For decentralized online patient control or the integration into everyday "consumables' such as drinking water, foods, hygienic articles, clothing, or for control of air conditioners in buildings and cars and swimming pools, a new generation of "autonomous' biosensors will emerge.
KW  - Biosensors
KW  - Molecularly imprinted polymers
KW  - Personalized medicine
Y1  - 2014
SN  - 978-3-642-54143-8; 978-3-642-54142-1
U6  - https://doi.org/10.1007/10_2013_251
SN  - 0724-6145
VL  - 140
SP  - 1
EP  - 28
PB  - Springer
CY  - Berlin
ER  - 
TY  - JOUR
A1  - Stöcklein, Walter F. M.
A1  - Warsinke, Axel
A1  - Scheller, Frieder W.
T1  - Organic solvent modified enzyme-liked immunoassay for the detection of triazine herbicides
Y1  - 1997
ER  - 
TY  - JOUR
A1  - Scheller, Frieder W.
A1  - Schubert, Frank
A1  - Federowitz, J.
T1  - Present state and frontiers in biosensorics
Y1  - 1997
ER  - 
TY  - JOUR
A1  - Stöcklein, Walter F. M.
A1  - Scheller, Frieder W.
T1  - Enzymes and antibodies in organic media : analytical applications
Y1  - 1997
ER  - 
TY  - JOUR
A1  - Wollenberger, Ursula
A1  - Lisdat, Fred
A1  - Scheller, Frieder W.
T1  - Enzymatic substrade recycling electrodes
Y1  - 1997
ER  - 
TY  - JOUR
A1  - Eremenko, A. V.
A1  - Makower, Alexander
A1  - Bauer, Christian G.
A1  - Kurochkin, I. N.
A1  - Scheller, Frieder W.
T1  - A bienzyme electrode for tyrosine containing peptides determination
Y1  - 1997
ER  - 
TY  - JOUR
A1  - Xie, B.
A1  - Tang, X.
A1  - Wollenberger, Ursula
A1  - Johansson, G.
A1  - Gorton, Lo
A1  - Scheller, Frieder W.
A1  - Danielsson, B.
T1  - Hybrid biosensor for simultaneous electrochemical and thermal detection
Y1  - 1997
ER  -