TY  - JOUR
A1  - Bier, Frank Fabian
A1  - Ehrentreich-Förster, Eva
A1  - Scheller, Frieder W.
A1  - Makower, Alexander
A1  - Eremenko, A. V.
A1  - Wollenberger, Ursula
A1  - Bauer, Christian G.
A1  - Pfeiffer, Dorothea
A1  - Micheel, Burkhard
T1  - Ultrasensitive biosensors
Y1  - 1996
ER  - 
TY  - JOUR
A1  - Ghindilis, A. L.
A1  - Makower, Alexander
A1  - Bauer, Christian G.
A1  - Bier, Frank Fabian
A1  - Scheller, Frieder W.
T1  - Determination of p-aminophenol and catecholamines at picomolar concentrations based on recycling enzyme amplification
Y1  - 1995
ER  - 
TY  - JOUR
A1  - Scheller, Frieder W.
A1  - Makower, Alexander
A1  - Bier, Frank Fabian
A1  - Wollenberger, Ursula
A1  - Ghindilis, A. L.
A1  - Eremenko, A. V.
A1  - Pfeiffer, Dorothea
T1  - Enzymsensoren zur Bestimmung subnanomolarer Konzentrationen
Y1  - 1995
ER  - 
TY  - JOUR
A1  - Bier, Frank Fabian
A1  - Ehrentreich-Förster, Eva
A1  - Makower, Alexander
A1  - Scheller, Frieder W.
T1  - An enzymatic amplification cycle for high sensitive immunoassay
Y1  - 1996
ER  - 
TY  - JOUR
A1  - Song, Min Ik
A1  - Bier, Frank Fabian
A1  - Scheller, Frieder W.
T1  - A method to detect superoxide radicals using teflon membrane and superoxide dismutase
Y1  - 1995
ER  - 
TY  - JOUR
A1  - Scheller, Frieder W.
A1  - Bier, Frank Fabian
A1  - Pfeiffer, Dorothea
T1  - Biosensoren : Grundlagen und Anwendungen
Y1  - 1995
ER  - 
TY  - JOUR
A1  - Scheller, Frieder W.
A1  - Makower, Alexander
A1  - Ghindilis, A. L.
A1  - Bier, Frank Fabian
A1  - Ehrentreich-Förster, Eva
A1  - Wollenberger, Ursula
A1  - Bauer, Christian G.
A1  - Micheel, Burkhard
A1  - Pfeiffer, Dorothea
A1  - Szeponik, Jan
A1  - Michael, N.
A1  - Kaden, H.
T1  - Enzyme sensors for subnanomolar concentrations
Y1  - 1995
ER  - 
TY  - JOUR
A1  - Jin, Wen
A1  - Wollenberger, Ursula
A1  - Bier, Frank Fabian
A1  - Scheller, Frieder W.
T1  - Construction and characterization of multi-layer-enzyme electrode : covalent binding of quinoprotein glucose dehydrogenase onto gold electrodes
Y1  - 1995
ER  - 
TY  - GEN
A1  - Bier, Frank Fabian
A1  - Yin, Wen
A1  - Kleinjung, Frank
A1  - Scheller, Frieder W.
T1  - Molekulare Schichten zur Analyse biochemischer Bindungen und Umsatzreaktionen
Y1  - 1995
ER  - 
TY  - JOUR
A1  - Kleinjung, Frank
A1  - Bier, Frank Fabian
A1  - Scheller, Frieder W.
T1  - Messungen an Nukleinsäuren mittels evaneszenten Feldes
Y1  - 1995
ER  - 
TY  - JOUR
A1  - Bier, Frank Fabian
A1  - Scheller, Frieder W.
A1  - Klingbeil, Mandy
A1  - Oßwald, U.
T1  - Biosensoren und Teststreifen für die Umwelt- und Lebensmittelanalytik : eine Übersicht
Y1  - 1993
ER  - 
TY  - JOUR
A1  - Scheller, Frieder W.
A1  - Bier, Frank Fabian
A1  - Neumann, B.
T1  - Bioindikation in aquatischen Ökosystemen : Bioindikation in limnischen und küstennahen Ökosystemen ; Grundlagen, Verfahren und Methoden
Y1  - 1994
PB  - Fischer
CY  - Jena
ER  - 
TY  - JOUR
A1  - Dechtrirat, Decha
A1  - Gajovic-Eichelmann, Nenad
A1  - Wojcik, Felix
A1  - Hartmann, Laura
A1  - Bier, Frank Fabian
A1  - Scheller, Frieder W.
T1  - Electrochemical displacement sensor based on ferrocene boronic acid tracer and immobilized glycan for saccharide binding proteins and E. coli
JF  - Biosensors and bioelectronics : the principal international journal devoted to research, design development and application of biosensors and bioelectronics
N2  - Pathogens such as viruses and bacteria use their envelope proteins and their adhesin lectins to recognize the glycan residues presented on the cell surface of the target tissues. This principle of recognition is used in a new electrochemical displacement sensor for the protein concanavalin A (ConA). A gold electrode was first modified with a self-assembled monolayer of a thiolated mannose/OEG conjugate and a ferrocene boroxol derivative was pre-assembled as reporter molecule onto the mannose surface. The novel tracer molecule based on a 2-hydroxymethyl phenyl boronic acid derivative binds even at neutral pH to the saccharides which could expand the application towards biological samples (i.e., urine and feces). Upon the binding of ConA, the tracer was displaced and washed away from the sensor surface leading to a decrease in the electrochemical signal. Using square wave voltammetry (SWV), the concentration of ConA in the sample solution could be determined in the dynamic concentration range established from 38 nmol L-1 to 5.76 mu mol L-1 with a reproducible detection limit of 1 mu g mL(-1) (38 nmol L-1) based on the signal-to-noise ratio (S/N=3) with fast response of 15 min. The new reporter molecule showed a reduced non-specific displacement by BSA and ribonuclease A. The sensor was also successfully transferred to the first proof of principle for the detection of Escherichia coli exhibiting a detection limit of approximately 6 x 102 cells/mL Specificity of the displacement by target protein ConA and E. coli was demonstrated since the control proteins (i.e., BSA and RNaseA) and the control E. coli strain, which lack of type 1 fimbriae, were ineffective. (C) 2014 Elsevier B.V. All rights reserved.
KW  - Ferrocene benzoboroxol biosensor
KW  - Concanavalin A
KW  - Displacement
KW  - Escherichia coli
KW  - Ferrocene boronic acid
KW  - Self-assembled monolayer
Y1  - 2014
U6  - https://doi.org/10.1016/j.bios.2014.02.028
SN  - 0956-5663
SN  - 1873-4235
VL  - 58
SP  - 1
EP  - 8
PB  - Elsevier
CY  - Oxford
ER  - 
TY  - JOUR
A1  - Scheller, Frieder W.
A1  - Yarman, Aysu
A1  - Bachmann, Till
A1  - Hirsch, Thomas
A1  - Kubick, Stefan
A1  - Renneberg, Reinhard
A1  - Schumacher, Soeren
A1  - Wollenberger, Ursula
A1  - Teller, Carsten
A1  - Bier, Frank Fabian
ED  - Gu, MB
ED  - Kim, HS
T1  - Future of biosensors: a personal view
JF  - Advances in biochemical engineering, biotechnology
JF  - Advances in Biochemical Engineering-Biotechnology
N2  - Biosensors representing the technological counterpart of living senses have found routine application in amperometric enzyme electrodes for decentralized blood glucose measurement, interaction analysis by surface plasmon resonance in drug development, and to some extent DNA chips for expression analysis and enzyme polymorphisms. These technologies have already reached a highly advanced level and need minor improvement at most. The dream of the "100-dollar' personal genome may come true in the next few years provided that the technological hurdles of nanopore technology or of polymerase-based single molecule sequencing can be overcome. Tailor-made recognition elements for biosensors including membrane-bound enzymes and receptors will be prepared by cell-free protein synthesis. As alternatives for biological recognition elements, molecularly imprinted polymers (MIPs) have been created. They have the potential to substitute antibodies in biosensors and biochips for the measurement of low-molecular-weight substances, proteins, viruses, and living cells. They are more stable than proteins and can be produced in large amounts by chemical synthesis. Integration of nanomaterials, especially of graphene, could lead to new miniaturized biosensors with high sensitivity and ultrafast response. In the future individual therapy will include genetic profiling of isoenzymes and polymorphic forms of drug-metabolizing enzymes especially of the cytochrome P450 family. For defining the pharmacokinetics including the clearance of a given genotype enzyme electrodes will be a useful tool. For decentralized online patient control or the integration into everyday "consumables' such as drinking water, foods, hygienic articles, clothing, or for control of air conditioners in buildings and cars and swimming pools, a new generation of "autonomous' biosensors will emerge.
KW  - Biosensors
KW  - Molecularly imprinted polymers
KW  - Personalized medicine
Y1  - 2014
SN  - 978-3-642-54143-8; 978-3-642-54142-1
U6  - https://doi.org/10.1007/10_2013_251
SN  - 0724-6145
VL  - 140
SP  - 1
EP  - 28
PB  - Springer
CY  - Berlin
ER  - 
TY  - JOUR
A1  - Stöcklein, Walter F. M.
A1  - Warsinke, Axel
A1  - Scheller, Frieder W.
T1  - Organic solvent modified enzyme-liked immunoassay for the detection of triazine herbicides
Y1  - 1997
ER  - 
TY  - JOUR
A1  - Scheller, Frieder W.
A1  - Schubert, Frank
A1  - Federowitz, J.
T1  - Present state and frontiers in biosensorics
Y1  - 1997
ER  - 
TY  - JOUR
A1  - Stöcklein, Walter F. M.
A1  - Scheller, Frieder W.
T1  - Enzymes and antibodies in organic media : analytical applications
Y1  - 1997
ER  - 
TY  - JOUR
A1  - Wollenberger, Ursula
A1  - Lisdat, Fred
A1  - Scheller, Frieder W.
T1  - Enzymatic substrade recycling electrodes
Y1  - 1997
ER  - 
TY  - JOUR
A1  - Eremenko, A. V.
A1  - Makower, Alexander
A1  - Bauer, Christian G.
A1  - Kurochkin, I. N.
A1  - Scheller, Frieder W.
T1  - A bienzyme electrode for tyrosine containing peptides determination
Y1  - 1997
ER  - 
TY  - JOUR
A1  - Xie, B.
A1  - Tang, X.
A1  - Wollenberger, Ursula
A1  - Johansson, G.
A1  - Gorton, Lo
A1  - Scheller, Frieder W.
A1  - Danielsson, B.
T1  - Hybrid biosensor for simultaneous electrochemical and thermal detection
Y1  - 1997
ER  - 
TY  - JOUR
A1  - Ehrentreich-Förster, Eva
A1  - Scheller, Frieder W.
A1  - McNeil, C. J.
T1  - Biosensor zur in vivo Messung von Superoxidradikalen
Y1  - 1997
ER  - 
TY  - JOUR
A1  - Szeponik, Jan
A1  - Möller, B.
A1  - Pfeiffer, Dorothea
A1  - Lisdat, Fred
A1  - Wollenberger, Ursula
A1  - Makower, Alexander
A1  - Scheller, Frieder W.
T1  - Ultrasensitive bienzyme sensor for adrenaline
Y1  - 1997
ER  - 
TY  - JOUR
A1  - Markower, Alexander
A1  - Wollenberger, Ursula
A1  - Hörtnagel, H.
A1  - Pfeiffer, Dorothea
A1  - Scheller, Frieder W.
T1  - Catecholamine detection using enzymatic amplification
Y1  - 1997
ER  - 
TY  - JOUR
A1  - Welzel, H.-P.
A1  - Kossmehl, G.
A1  - Engelmann, G.
A1  - Neumann, B.
A1  - Wollenberger, Ursula
A1  - Scheller, Frieder W.
T1  - Electrochemical polymerization of functionalized thiohene derivatives for immobilization of proteins
Y1  - 1997
ER  - 
TY  - JOUR
A1  - Gajovic, Nenad
A1  - Warsinke, Axel
A1  - Scheller, Frieder W.
T1  - Comparsion of two enzyme sequences for a novel L-malate biosensor
Y1  - 1997
ER  - 
TY  - JOUR
A1  - Katterle, Martin
A1  - Wollenberger, Ursula
A1  - Scheller, Frieder W.
T1  - Electrochemistry of hemoglobin at modified silver electrodes is not a redox-process of iron protoporhyrin IX
Y1  - 1997
ER  - 
TY  - JOUR
A1  - Ehrentreich-Förster, Eva
A1  - Scheller, Frieder W.
T1  - Charakterisierung antioxidativer Substanzen mit einem Superoxidsensor
Y1  - 1997
ER  - 
TY  - JOUR
A1  - Kulys, J.
A1  - Drungiliene, A.
A1  - Wollenberger, Ursula
A1  - Krikstopaitis, K.
A1  - Scheller, Frieder W.
T1  - Electroanalytical determination of peroxidases and laccases on carbon paste electrodes
Y1  - 1997
ER  - 
TY  - JOUR
A1  - Kleinjung, Frank
A1  - Beier, Frank F.
A1  - Warsinke, Axel
A1  - Scheller, Frieder W.
T1  - Fibre-optic genosensor for specific determination of femtomolar DNA oligomers
Y1  - 1997
ER  - 
TY  - JOUR
A1  - Pfeiffer, Dorothea
A1  - Yang, L.
A1  - Scheller, Frieder W.
A1  - Kissinger, P. T.
T1  - Continous measurement of lactate in microdialysate
Y1  - 1997
ER  - 
TY  - JOUR
A1  - Wollenberger, Ursula
A1  - Drungiliene, A.
A1  - Stöcklein, Walter F. M.
A1  - Kulys, J.
A1  - Scheller, Frieder W.
T1  - Direct electrocatalytic determination of dissolved peroxidases
Y1  - 1996
ER  - 
TY  - JOUR
A1  - Kaisheva, A.
A1  - Iliev, I.
A1  - Kazareva, R.
A1  - Christov, S.
A1  - Wollenberger, Ursula
A1  - Scheller, Frieder W.
T1  - Enzyme/gas diffusion electrodes for determination of phenol
Y1  - 1996
ER  - 
TY  - JOUR
A1  - Makower, Alexander
A1  - Eremenko, A. V.
A1  - Streffer, Katrin
A1  - Wollenberger, Ursula
A1  - Scheller, Frieder W.
T1  - Tyrosinase-glucose dehydrogenase substrate-recycling biosensor : a highly sensitive measurement of phenolic compounds
Y1  - 1996
ER  - 
TY  - JOUR
A1  - Spricigo, Roberto
A1  - Richter, Claudia
A1  - Leimkühler, Silke
A1  - Gorton, Lo
A1  - Scheller, Frieder W.
A1  - Wollenberger, Ursula
T1  - Sulfite biosensor based on osmium redox polymer wired sulfite oxidase
N2  - A biosensor, based on a redoxactive osmium polymer and sulfite oxidase on screen-printed electrodes, is presented here as a promising method for the detection of sulfite. A catalytic oxidative current was generated when a sample containing sulfite was pumped over the carbon screen-printed electrode modified with osmium redox polymer wired sulfite oxidase. A stationary value was reached after approximately 50 s and a complete measurement lasted no more than 3 min. The electrode polarized at -0.1 V (vs. Ag vertical bar AgCl 1M KCl) permits minimizing the influence of interfering substances, since these compounds can be unspecific oxidized at higher potentials. Because of the good stability of the protein film on the electrode surface, a well functioning biosensor-flow system was possible to construct. The working stability and reproducibility were further enhanced by the addition of bovine serum albumin generating a more long-term stable and biocompatible protein environment. The optimized biosensor showed a stable signal for more than a week of operation and a coefficient of variation of 4.8% for 12 successive measurements. The lower limit of detection of the sensor was 0.5 mu M sulfite and the response was linear until 100 mu M. The high sensitivity permitted a 1:500 dilution of wine samples. The immobilization procedure and the operational conditions granted minimized interferences. Additionally, repeating the immobilization procedure to form several layers of wired SO further increased the sensitivity of such a sensor. Finally. the applicability of the developed sulfite biosensor was tested on real samples, such as white and red wines.
Y1  - 2010
UR  - http://www.sciencedirect.com/science/journal/09277757
U6  - https://doi.org/10.1016/j.colsurfa.2009.09.001
SN  - 0927-7757
ER  - 
TY  - JOUR
A1  - Pfeiffer, Dorothea
A1  - Scheller, Frieder W.
A1  - McNeil, C. J.
A1  - Schulmeister, Thomas
T1  - Cascade-like exponential substrate amplification in enzyme sensors
Y1  - 1995
ER  - 
TY  - JOUR
A1  - Paeschke, Manfred
A1  - Hintsche, Rainer
A1  - Wollenberger, Ursula
A1  - Jin, Wen
A1  - Scheller, Frieder W.
T1  - Dynamic redox recycling of cytochrome c
Y1  - 1995
SN  - 0022-0728
ER  - 
TY  - JOUR
A1  - Scheller, Frieder W.
A1  - Pfeiffer, Dorothea
T1  - Biosensoren : ein wirtschaftlicher Faktor für die Zukunft
Y1  - 1994
ER  - 
TY  - JOUR
A1  - Scheller, Frieder W.
T1  - Biosensoren : Konzepte, Technologien, Perspektiven
Y1  - 1994
ER  - 
TY  - JOUR
A1  - Scheller, Frieder W.
A1  - Kirstein, Dieter
A1  - Pfeiffer, Dorothea
T1  - Biosensoren, Konzepte, Technologien, Perspektiven
Y1  - 1994
ER  - 
TY  - JOUR
A1  - Scheller, Frieder W.
A1  - Heiduschka, P.
T1  - Preparation of an electrode surface with a high density of binding sites by an electrochemical reduction of a poly (nitrophenol) film
Y1  - 1994
ER  - 
TY  - JOUR
A1  - Schmid, Rolf D.
A1  - Scheller, Frieder W.
T1  - Biosensoren
Y1  - 1994
ER  - 
TY  - JOUR
A1  - Lisdat, Fred
A1  - Dronov, Roman
A1  - Möhwald, Helmuth
A1  - Scheller, Frieder W.
A1  - Kurth, Dirk G.
T1  - Self-assembly of electro-active protein architectures on electrodes for the construction of biomimetic signal chains
N2  - The layer-by-layer adsorption technique based on the consecutive deposition of oppositely charged species is for the preparation of protein multilayers with fully electro-active protein molecules. The methodology was established with cytochrome c and the polyelectrolyte sulfonated polyaniline (PASA). The technique is also useful for the construction of bi-protein architectures confining protein-protein communication to an electrode. Following natural examples of protein complexes with defined signal transfer, cytochrome c was arranged with enzymes such as xanthine oxidase, bilirubin oxidase, laccase, and sulfite oxidase in self-assembled multilayer architectures. Thus, biomimetic signal chains from the enzyme substrate via the enzyme and cytochrome c towards the electrode can be established. Communication between proteins immobilised in multiple layers on the electrode can be achieved by in situ generation of small shuttle molecules or more advantageously by direct interprotein electron transfer. This allows the construction of new sensing electrodes, the properties of which can be tuned by the number of deposited protein layers. The mechanism of electron transfer within such protein assemblies on gold electrodes will be discussed.
Y1  - 2009
UR  - http://xlink.rsc.org/jumptojournal.cfm?journal_code=CC
U6  - https://doi.org/10.1039/B813559b
SN  - 1359-7345
ER  - 
TY  - JOUR
A1  - Loew, Noya
A1  - Wollenberger, Ursula
A1  - Scheller, Frieder W.
A1  - Katterle, Martin
T1  - Direct electrochemistry and spectroelectrochemistry of osmium substituted horseradish peroxidase
N2  - In this contribution the substitution of the central protoporphyrin IX iron complex of horseradish peroxidase by the respective osmium porphyrin complex is described. The direct electrochemical reduction of the Os containing horseradish peroxidase (OsHRP) was achieved at ITO and modified glassy carbon electrodes and in combination with spectroscopy revealed the three redox couples (OsHRP)-H-III/(OsHRP)-H-IV, (OsHRP)-H-IV/(OsHRP)-H-V and (OsHRP)-H-V/ (OsHRP)-H-VI. The midpoint potentials differ dependent on the electrode material used with E-1/2 (Os-III/IV) of -0.4 V (ITO) and -0.25 V (GC), E-1/2 (Os-IV/V) of -0.16 V (ITO) and +0.10 V (GC), and E-1/2 (Os-V/VI)of +018 V (ITO), respectively Moreover, with immobilised OsHRP the direct electrocatalytic reduction of hydrogen peroxide and tert-butyl hydroperoxide was observed. In comparison to electrodes modified with native HRP the sensitivity of the OsHRP-electrode for tert-butyl hydroperoxide is higher.
Y1  - 2009
UR  - http://www.sciencedirect.com/science/journal/15675394
U6  - https://doi.org/10.1016/j.bioelechem.2009.03.015
SN  - 1567-5394
ER  - 
TY  - JOUR
A1  - Schulmeister, Thomas
A1  - Scheller, Frieder W.
T1  - The mathematics of exponential signal amplification in amperometric three enzyme electrodes
Y1  - 1996
ER  - 
TY  - JOUR
A1  - Stancik, L.
A1  - Macholán, L.
A1  - Scheller, Frieder W.
T1  - Biosensing of tyrosinase inhibitors in nonaqueous solvents
Y1  - 1995
ER  - 
TY  - JOUR
A1  - Stöcklein, Walter F. M.
A1  - Scheller, Frieder W.
A1  - Abuknesha, Rhamadan
T1  - Effects of organic solvents on semicontinuous immunochemical detection of coumarin derivatives
Y1  - 1995
ER  - 
TY  - JOUR
A1  - Stöcklein, Walter F. M.
A1  - Scheller, Frieder W.
T1  - Einsatz von Enzymen und Antikörpern in organischen Lösungsmitteln
Y1  - 1995
ER  - 
TY  - JOUR
A1  - Wollenberger, Ursula
A1  - Hintsche, R.
A1  - Scheller, Frieder W.
T1  - Biosensors for analytical microsystems
Y1  - 1995
ER  - 
TY  - JOUR
A1  - Eremenko, A. V.
A1  - Makower, Alexander
A1  - Scheller, Frieder W.
T1  - Measurement of nanomolar diphenols by substrate recycling coupled to a pH- sensitive electrode
Y1  - 1995
ER  - 
TY  - JOUR
A1  - Gajovic, Nenad
A1  - Warsinke, Axel
A1  - Scheller, Frieder W.
T1  - A novel multienzyme electrode for the determination of citrate
Y1  - 1995
ER  - 
TY  - JOUR
A1  - Ghindilis, A. L.
A1  - Makower, Alexander
A1  - Scheller, Frieder W.
T1  - Laccase - glucose dehydrogenase recycling enzyme electrode based on potentiometric mediatorless electrocatalytic detection
Y1  - 1995
ER  - 
TY  - JOUR
A1  - Ghindilis, A. L.
A1  - Makower, Alexander
A1  - Scheller, Frieder W.
T1  - Nanomolar determination of the ferrocene derivatives using a recycling enzyme electrode : development of the redox label immunoassay
Y1  - 1995
ER  - 
TY  - JOUR
A1  - Ghindilis, A. L.
A1  - Makower, Alexander
A1  - Scheller, Frieder W.
T1  - Potentiometric enzyme electrodes based on substrate recycling and mediatorless bioelectrocatalysis
Y1  - 1995
ER  - 
TY  - JOUR
A1  - Lisdat, Fred
A1  - Wollenberger, Ursula
A1  - Paeschke, Manfred
A1  - Scheller, Frieder W.
T1  - Sensitive catecholamine measurement using a monoenzymatic recycling system
Y1  - 1998
ER  - 
TY  - JOUR
A1  - Stancík, L.
A1  - Macholán, L.
A1  - Pluhacek, I.
A1  - Scheller, Frieder W.
T1  - Biosensing of rapeseed glucosinolates using amperometric enzyme electrodes based on membrane-bound glucose oxidase or tyrosinase
Y1  - 1995
ER  - 
TY  - JOUR
A1  - Eremenko, A. V.
A1  - Makower, Alexander
A1  - Jin, Wen
A1  - Rüger, P.
A1  - Scheller, Frieder W.
T1  - Biosensor based on an enzyme modified electrode for highly - sensitive measurement of polyphenols
Y1  - 1995
ER  - 
TY  - JOUR
A1  - Iliev, I.
A1  - Kaisheva, A.
A1  - Scheller, Frieder W.
A1  - Pfeiffer, Dorothea
T1  - Amperometric gas-diffusion / enzyme electrode
Y1  - 1995
ER  - 
TY  - JOUR
A1  - Riedel, K.
A1  - Beyersdorf-Radeck, Baerbel
A1  - Neumann, B.
A1  - Scheller, Frieder W.
A1  - Schmid, Rolf D.
T1  - Microbial sensors for determination of aromatics and their chloro derivatives. Part III: Determination of chlorinated phenols using a biosensor containing Trichosporon beigelii (cutaneum)
Y1  - 1995
ER  - 
TY  - JOUR
A1  - Shumyantseva, V. V.
A1  - Ivanov, Y. D.
A1  - Bistolas, Nikitas
A1  - Scheller, Frieder W.
A1  - Archakov, Alexander I.
A1  - Wollenberger, Ursula
T1  - Direct electron transfer of cytochrome P450 2B4 at electrodes modified with non-ionic detergent and colloidal clay nanoparticles
N2  - A method for construction of biosensors with membranous cytochrome P450 isoenzymes was developed based on clay/ detergent/protein mixed films. Thin films of sodium montmorillonite colloid with incorporated cytochrome P450 2134 (CYP2B4) with nonionic detergent were prepared on glassy carbon electrodes. The modified electrodes were electrochemically characterized, and bio-electrocatalytic reactions were followed. CYP2B4 can be reduced fast on clay- modified glassy carbon electrodes in the presence of the nonionic detergent Tween 80. In anaerobic solutions, reversible oxidation and reduction is obtained with a formal potential between -0.292 and - 0.305 V vs Ag/AgCl 1 M KCl depending on the preparation of the biosensor. In air-saturated solution, bio-electrocatalytic reduction currents can be obtained with the CYP2B4-modified electrode on addition of typical substrates such as aminopyrine and benzphetamine. This reaction was suppressed when methyrapone, an inhibitor of P450 reactions, was present. Measurement of product formation also indicates the bioelectrocatialysis by CYP2B4
Y1  - 2004
ER  - 
TY  - JOUR
A1  - Frasca, Stefano
A1  - von Graberg, Till
A1  - Feng, Jiu-Ju
A1  - Thomas, Arne
A1  - Smarsly, Bernd M.
A1  - Weidinger, Inez M.
A1  - Scheller, Frieder W.
A1  - Hildebrandt, Peter
A1  - Wollenberger, Ursula
T1  - Mesoporous indium tin oxide as a novel platform for bioelectronics
N2  - Stable immobilization and reversible electrochemistry of cytochrome c in a tranparent indium tin oxide film with a well-defined mesoporosity (mpITO) is demonstrated. the transparency and good conductivity, in combination with the large surface area of mpITO, allow the incorporation of a high amount of elelctroactive biomolecules and their electrochemical and spectroscopic investigation. UV/Vis and resonance Raman spectroscopy, in combination with direct protein voltammetry are employed for the characterization of cytochrome c immobilized in the mpITO and reveal no perturbant of the structural of the integrity of the redox protein. The potential of this modified material as a biosensor detection of superoxide anions is also demonstrated.
Y1  - 2010
UR  - http://www3.interscience.wiley.com/journal/122208635/home
U6  - https://doi.org/10.1002/cctc.201000047
SN  - 1867-3880
ER  - 
TY  - JOUR
A1  - Stöcklein, Walter F. M.
A1  - Warsinke, Axel
A1  - Micheel, Burkhard
A1  - Höhne, Wolfgang
A1  - Woller, Jochen
A1  - Kempter, Gerhard
A1  - Scheller, Frieder W.
T1  - Characterization of a monoclonal antibody and its Fab fragment against diphenylurea hapten with BIA
Y1  - 1998
SN  - 3-8154-3540-4
ER  - 
TY  - JOUR
A1  - Warsinke, Axel
A1  - Stancik, L.
A1  - Macholán, L.
A1  - Pfeiffer, Dorothea
A1  - Scheller, Frieder W.
T1  - Biosensors for food analysis : application of biosensors to food requirements
Y1  - 1998
SN  - 0-85404-750-6
ER  - 
TY  - JOUR
A1  - Stöcklein, Walter F. M.
A1  - Warsinke, Axel
A1  - Micheel, Burkhard
A1  - Höhne, Wolfgang
A1  - Woller, Jochen
A1  - Kempter, Gerhard
A1  - Scheller, Frieder W.
T1  - Detection of diphenylurea derivatives with biospecific interaction analysis (BIA) : Kinetic investigations
Y1  - 1997
ER  - 
TY  - JOUR
A1  - Spricigo, Roberto
A1  - Dronov, Roman
A1  - Lisdat, Fred
A1  - Leimkühler, Silke
A1  - Scheller, Frieder W.
A1  - Wollenberger, Ursula
T1  - Electrocatalytic sulfite biosensor with human sulfite oxidase co-immobilized with cytochrome c in a polyelectrolyte-containing multilayer
N2  - An efficient electrocatalytic biosensor for sulfite detection was developed by co-immobilizing sulfite oxidase and cytochrome c with polyaniline sulfonic acid in a layer-by-layer assembly. QCM, UV-Vis spectroscopy and cyclic voltammetry revealed increasing loading of electrochemically active protein with the formation of multilayers. The sensor operates reagentless at low working potential. A catalytic oxidation current was detected in the presence of sulfite at the modified gold electrode, polarized at +0.1 V ( vs. Ag/AgCl 1 M KCl). The stability of the biosensor performance was characterized and optimized. A 17-bilayer electrode has a linear range between 1 and 60 mu M sulfite with a sensitivity of 2.19 mA M-1 sulfite and a response time of 2 min. The electrode retained a stable response for 3 days with a serial reproducibility of 3.8% and lost 20% of sensitivity after 5 days of operation. It is possible to store the sensor in a dry state for more than 2 months. The multilayer electrode was used for determination of sulfite in unspiked and spiked samples of red and white wine. The recovery and the specificity of the signals were evaluated for each sample.
Y1  - 2009
UR  - http://www.springerlink.com/content/100417
U6  - https://doi.org/10.1007/s00216-008-2432-y
SN  - 1618-2642
ER  - 
TY  - JOUR
A1  - Ehrentreich-Förster, Eva
A1  - Shishniashvili, D.
A1  - Song, Min Ik
A1  - Scheller, Frieder W.
T1  - Study of antioxidative substances by means of a ssuperoxide sensor
Y1  - 1998
ER  - 
TY  - JOUR
A1  - Kulys, J.
A1  - Drungiliene, A.
A1  - Wollenberger, Ursula
A1  - Scheller, Frieder W.
T1  - Membrane covered carbon paste electrode for the electrochemical determination of perioxidase and microperoxidase in a flow system
Y1  - 1998
ER  - 
TY  - JOUR
A1  - Jin, Wen
A1  - Bernhardt, Rita
A1  - Stöcklein, Walter F. M.
A1  - Scheller, Frieder W.
T1  - Direct electron transfer of adrenodoxin-a [2Fe-2S] protein-- and its mutants on modified gold electrode
Y1  - 1998
ER  - 
TY  - JOUR
A1  - Wollenberger, Ursula
A1  - Neumann, B.
A1  - Scheller, Frieder W.
T1  - Development of a biomimetic alkane sensor f
Y1  - 1998
ER  - 
TY  - JOUR
A1  - Scheller, Frieder W.
T1  - Biosensorik
Y1  - 1998
ER  - 
TY  - JOUR
A1  - Scheller, Frieder W.
A1  - Pfeiffer, Dorothea
A1  - Lisdat, Fred
A1  - Bauer, Christian G.
A1  - Gajovic, Nenad
T1  - Enzyme biosensors based on oxygen detection
Y1  - 1998
ER  - 
TY  - JOUR
A1  - Bogdanovskaya, V. A.
A1  - Fridman, Vadim
A1  - Tarasevich, M. R.
A1  - Scheller, Frieder W.
T1  - Bioelectrocatalysis by immobilized peroxidase : the reaction mechanism and the possibility of electroanalytical detection of both inhibitors and activators of enzyme
Y1  - 1994
ER  - 
TY  - JOUR
A1  - Kirstein, Dieter
A1  - Kirstein, Lincoln
A1  - Scheller, Frieder W.
A1  - Dieckmann, St.
A1  - Ronnenberg, J.
A1  - Beckmann, Dieter
A1  - Weckenbrock, E.
T1  - Elektroenzymatische Reduktion von Nitrat
Y1  - 1994
ER  - 
TY  - JOUR
A1  - Wollenberger, Ursula
A1  - Neumann, B.
A1  - Riedel, K.
A1  - Scheller, Frieder W.
T1  - Enzyme and microbial sensors for phosphate, phenols, pesticides and peroxides
Y1  - 1994
ER  - 
TY  - JOUR
A1  - Yarman, Aysu
A1  - Scheller, Frieder W.
T1  - Coupling biocatalysis with molecular imprinting in a biomimetic sensor
JF  - Angewandte Chemie : a journal of the Gesellschaft Deutscher Chemiker ; International edition
KW  - biomimetic sensors
KW  - electropolymers
KW  - enzymes
KW  - hierarchical structures
KW  - molecularly imprinted polymers
Y1  - 2013
U6  - https://doi.org/10.1002/anie.201305368
SN  - 1433-7851
SN  - 1521-3773
VL  - 52
IS  - 44
SP  - 11521
EP  - 11525
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Scheller, Frieder W.
A1  - Pfeiffer, Dorothea
T1  - Commercial devices based on amperometric biosensors
Y1  - 1997
ER  - 
TY  - JOUR
A1  - Schulmeister, Thomas
A1  - Rose, Jürgen
A1  - Scheller, Frieder W.
T1  - Mathematical modelling of exponential amplification in membrane-based enzyme sensors
Y1  - 1997
ER  - 
TY  - JOUR
A1  - Warsinke, Axel
A1  - Benkert, Alexander
A1  - Scheller, Frieder W.
T1  - Biomolecular modules for creatinine determination
Y1  - 1996
ER  - 
TY  - JOUR
A1  - Stöcklein, Walter F. M.
A1  - Scheller, Frieder W.
T1  - Laccase : a marker enzyme for solvent modified immunoassays
Y1  - 1996
ER  - 
TY  - JOUR
A1  - Halámek, Jan
A1  - Wollenberger, Ursula
A1  - Stöcklein, Walter F. M.
A1  - Scheller, Frieder W.
T1  - Development of a biosensor for glycated hemoglobin
N2  - The development of an electrochemical piezoelectric sensor for the detection of glycated hemoglobin is presented. The total hemoglobin (Hb) content is monitored with a mass-sensitive quartz crystal modified with surfactants, and the glycated fraction of the immobilized Hb is determined by subsequent voltarnmetric measurement of the coupled ferroceneboronic acid. Different modifications of the sensor were tested for their hemoglobin binding ability. Deoxycholate (DOCA) was found to be the most suitable among the examined modifiers. Piezoelectric quartz crystals with gold electrodes were modified with DOCA by covalent binding to a pre-formatted 4-aminothiophenol monolayer. The properties of the Hb binding to DOCA and the pH effect on this interaction were studied. In the proposed assay for glycated hemoglobin at first an Hb sample is incubated with ferroceneboronic acid (FcBA), which binds to the fructosyl residue of the glycated Hb. Then this preincubated Hb sample is allowed to interact with the DOCA-modified piezoelectric quartz crystal. The binding is monitored by quartz crystal nanobalance QCN). The amount of FcBA present on the sensor surface is determined by square wave voltammetry. The binding of FcBA results in well-defined peaks with an EO' of +200 mV versus Ag/AgC1 (1 M KC1). The peak height depends on the degree of glycated Hb in the sample ranging from 0% to 20% of total Hb. The regeneration of the sensing surface is achieved by pepsin digestion of the deposited Hb. Thus the sensor can be re-used more than 30 times.
Y1  - 2007
UR  - http://www.sciencedirect.com/science/journal/00134686
U6  - https://doi.org/10.1016/j.electacta.2007.03.059
SN  - 0013-4686
ER  - 
TY  - JOUR
A1  - Halámek, Jan
A1  - Wollenberger, Ursula
A1  - Stöcklein, Walter F. M.
A1  - Warsinke, Axel
A1  - Scheller, Frieder W.
T1  - Signal amplification in immunoassays using labeling via boronic acid binding to the sugar moiety of immunoglobulin G : proof of concept for glycated hemoglobin
N2  - A novel electrochemical immunoassay based on the multiple affinity labeling of the indicator antibody with an electro-active tag is presented. The concept is illustrated for the determination of the glycated hemoglobin HbA1c in hemoglobin samples. Hemoglobin is adsorbed to the surfactant-modified surface of a piezoelectric quartz crystal. Whereas the quartz crystal nanobalance is used to validate the total Hb binding, the HbA1c on the sensor surface is recognized by an antibody and quantified electrochemically after the sugar moieties of the antibody have been labeled in-situ with ferroceneboronic acid. The sensitivity of this sensor is about threefold higher than the sensitivity of a hemoglobin sensor, where the ferroceneboronic acid is bound directly to HbA1c.
Y1  - 2007
UR  - http://www.informaworld.com/openurl?genre=journal&issn=0003-2719
U6  - https://doi.org/10.1080/00032710701327096
SN  - 0003-2719
ER  - 
TY  - JOUR
A1  - Scheller, Frieder W.
T1  - Tribute to Guenter Gauglitz (Editorial)
Y1  - 2009
UR  - http://www.springerlink.com/content/100417
U6  - https://doi.org/10.1007/s00216-008-2548-0
SN  - 1618-2642
ER  - 
TY  - JOUR
A1  - Yarman, Aysu
A1  - Wollenberger, Ursula
A1  - Scheller, Frieder W.
T1  - Sensors based on cytochrome P450 and CYP mimicking systems
JF  - ELECTROCHIMICA ACTA
N2  - Cytochrome P450 enzymes (CYPs) act on more than 90 percent of all drugs currently on the market. The catalytic cycle requires electron supply to the heme iron in the presence of oxygen. Electrochemistry allows to characterise the reaction mechanism of these redox enzymes by observing the electron transfer in real time. According to the number of publications on protein electrochemistry CYP has the third position after glucose oxidase and cytochrome c. CYP based enzyme electrodes for the quantification of drugs, metabolites or pesticides have been developed using different iso-enzymes. A crucial step in the sensor development is the efficiency of coupling the biocatalytic systems with the electrode is. In the 1970s the direct electron transfer of heme and heme peptides called microperoxidases (MPs) was used as model of oxidoreductases. They exhibit a broad substrate spectrum including hydroxylation of selected aromatic substrates, demethylation and epoxidation by means of hydrogen peroxide. It overlaps with that of P450 making heme and MPs to alternate recognition elements in biosensors for the detection of typical CYP substrates. In these enzyme electrodes the signal is generated by the conversion of all substrates thus representing in complex media an overall parameter. By combining the biocatalytic substrate conversion with selective binding to a molecularly imprinted polymer layer the specificity has been improved. Here we discuss different approaches of biosensors based on CYP, microperoxidases and catalytically active MIPs and discuss their potential as recognition elements in biosensors. The performance of these sensors and their further development are discussed. (C) 2013 Elsevier Ltd. All rights reserved.
KW  - Cytochrome P450
KW  - Microperoxidases
KW  - Catalytically active molecularly imprinted polymers
KW  - Biosensors
KW  - Personalised medicine
Y1  - 2013
U6  - https://doi.org/10.1016/j.electacta.2013.03.154
SN  - 0013-4686
SN  - 1873-3859
VL  - 110
SP  - 63
EP  - 72
PB  - PERGAMON-ELSEVIER SCIENCE LTD
CY  - OXFORD
ER  - 
TY  - JOUR
A1  - Lehmann, Claudia
A1  - Wollenberger, Ursula
A1  - Brigelius-Flohé, Regina
A1  - Scheller, Frieder W.
T1  - Bioelectrocatalysis by a selenoenzyme
Y1  - 1998
ER  - 
TY  - JOUR
A1  - Kirstein, Dieter
A1  - Kirstein, Lincoln
A1  - Scheller, Frieder W.
A1  - Borcherding, H.
T1  - Amperometric nitrate biosensors on the basis of Pseudomonas stutzeri nitrate reductase
Y1  - 1998
ER  - 
TY  - JOUR
A1  - Scheller, Frieder W.
T1  - Neue Dimensionen der Biosensorik
Y1  - 1998
ER  - 
TY  - JOUR
A1  - Jin, Wen
A1  - Wollenberger, Ursula
A1  - Scheller, Frieder W.
T1  - PQQ as redox shuttle for quinoprotein glucose dehydrogenase
Y1  - 1998
ER  - 
TY  - JOUR
A1  - Stöcklein, Walter F. M.
A1  - Warsinke, Axel
A1  - Micheel, Burkhard
A1  - Kempter, Gerhard
A1  - Höhne, Wolfgang
A1  - Scheller, Frieder W.
T1  - Diphenylurea hapten sensing with a monoclonal antibody and its Fab fragment : kinetic and thermodynamic investigations
Y1  - 1998
ER  - 
TY  - JOUR
A1  - Eremenko, Arkadi V.
A1  - Bauer, Christian G.
A1  - Makower, Alexander
A1  - Kanne, Beate
A1  - Baumgarten, Horst
A1  - Scheller, Frieder W.
T1  - The development of a non-competitive immunoenzymometric Assay (IEMA) of cocaine
Y1  - 1998
ER  - 
TY  - JOUR
A1  - Streffer, Katrin
A1  - Kaatz, Helvi
A1  - Bauer, Christian G.
A1  - Makower, Alexander
A1  - Schulmeister, Thomas
A1  - Scheller, Frieder W.
A1  - Peter, Martin G.
A1  - Wollenberger, Ursula
T1  - Application of a sensitive catechol detector for determination of tyrosinase inhibitors
Y1  - 1998
ER  - 
TY  - JOUR
A1  - Huang, T.
A1  - Warsinke, Axel
A1  - Kuwana, T.
A1  - Scheller, Frieder W.
T1  - The determination of L-phenylalanine based on a novel NADH-detecting biosensor
Y1  - 1998
ER  - 
TY  - JOUR
A1  - Bauer, Christian G.
A1  - Eremenko, A. V.
A1  - Kühn, A.
A1  - Kürzinger, K.
A1  - Markower, Alexander
A1  - Scheller, Frieder W.
T1  - Automated amplifield flow immunoassay for cocaine
Y1  - 1998
ER  - 
TY  - JOUR
A1  - Wollenberger, Ursula
A1  - Jin, Wen
A1  - Bernhardt, Rita
A1  - Lehmann, Claudia
A1  - Stöcklein, Walter F. M.
A1  - Brigelius-Flohé, Regina
A1  - Scheller, Frieder W.
T1  - Funktionalisierung von Elektroden für den direkten heterogenen Elektrotransfer
Y1  - 1998
ER  - 
TY  - JOUR
A1  - Gajovic, Nenad
A1  - Warsinke, Axel
A1  - Scheller, Frieder W.
T1  - A bienzyme electrode for L-malate based on a novel and general design
Y1  - 1998
ER  - 
TY  - JOUR
A1  - Lisdat, Fred
A1  - Ho, Wah O.
A1  - Wollenberger, Ursula
A1  - Scheller, Frieder W.
A1  - Richter, Torsten
A1  - Bilitewski, Ursula
T1  - Recycling systems based on screen-printed electrodes
Y1  - 1998
ER  - 
TY  - JOUR
A1  - Baeumner, Antje J.
A1  - Gauglitz, Guenter
A1  - Scheller, Frieder W.
T1  - Focus on bioanalysis
N2  - Editoria
Y1  - 2010
UR  - http://www.springerlink.com/content/100417
U6  - https://doi.org/10.1007/s00216-010-4203-9
SN  - 1618-2642
ER  - 
TY  - JOUR
A1  - Bosserdt, Maria
A1  - Gajovic-Eichelman, Nenad
A1  - Scheller, Frieder W.
T1  - Modulation of direct electron transfer of cytochrome c by use of a molecularly imprinted thin film
JF  - Analytical & bioanalytical chemistry
N2  - We describe the preparation of a molecularly imprinted polymer film (MIP) on top of a self-assembled monolayer (SAM) of mercaptoundecanoic acid (MUA) on gold, where the template cytochrome c (cyt c) participates in direct electron transfer (DET) with the underlying electrode. To enable DET, a non-conductive polymer film is electrodeposited from an aqueous solution of scopoletin and cyt c on to the surface of a gold electrode previously modified with MUA. The electroactive surface concentration of cyt c was 0.5 pmol cm(-2). In the absence of the MUA layer, no cyt c DET was observed and the pseudo-peroxidatic activity of the scopoletin-entrapped protein, assessed via oxidation of Ampliflu red in the presence of hydrogen peroxide, was only 30 % of that for the MIP on MUA. This result indicates that electrostatic adsorption of cyt c by the MUA-SAM substantially increases the surface concentration of cyt c during the electrodeposition step, and is a prerequisite for the productive orientation required for DET. After template removal by treatment with sulfuric acid, rebinding of cyt c to the MUA-MIP-modified electrode occurred with an affinity constant of 100,000 mol(-1) L, a value three times higher than that determined by use of fluorescence titration for the interaction between scopoletin and cyt c in solution. The DET of cyt c in the presence of myoglobin, lysozyme, and bovine serum albumin (BSA) reveals that the MIP layer suppresses the effect of competing proteins.
KW  - Cytochrome c
KW  - Molecularly imprinted polymer film
KW  - Mercaptoundecanoic acid
KW  - Direct electron transfer
KW  - Scopoletin (7-hydroxy-6-methoxycoumarin)
Y1  - 2013
U6  - https://doi.org/10.1007/s00216-013-7009-8
SN  - 1618-2642
VL  - 405
IS  - 20
SP  - 6437
EP  - 6444
PB  - Springer
CY  - Heidelberg
ER  - 
TY  - JOUR
A1  - Scheller, Frieder W.
T1  - New recognition elements for bioanalytics
Y1  - 1996
ER  - 
TY  - JOUR
A1  - Pfeiffer, Dorothea
A1  - Schubert, Frank
A1  - Wollenberger, Ursula
A1  - Scheller, Frieder W.
T1  - Electrochemical sensors : enzyme electrodes and field effect transistors
Y1  - 1996
ER  - 
TY  - JOUR
A1  - Wollenberger, Ursula
A1  - Scheller, Frieder W.
T1  - Enzyme activation for activator and enzyme activity measurement
Y1  - 1993
ER  - 
TY  - JOUR
A1  - Wollenberger, Ursula
A1  - Neumann, B.
A1  - Scheller, Frieder W.
T1  - Enzyme and microbial sensors for environmental Monitoring
Y1  - 1993
ER  -