TY  - GEN
A1  - Ozcelikay, Goksu
A1  - Kurbanoglu, Sevinc
A1  - Zhang, Xiaorong
A1  - Söz, Çağla Kosak
A1  - Wollenberger, Ulla
A1  - Ozkan, Sibel A.
A1  - Yarman, Aysu
A1  - Scheller, Frieder W.
T1  - Electrochemical MIP Sensor for Butyrylcholinesterase
T2  - Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe
N2  - Molecularly imprinted polymers (MIPs) mimic the binding sites of antibodies by substituting the amino acid-scaffold of proteins by synthetic polymers. In this work, the first MIP for the recognition of the diagnostically relevant enzyme butyrylcholinesterase (BuChE) is presented. The MIP was prepared using electropolymerization of the functional monomer o-phenylenediamine and was deposited as a thin film on a glassy carbon electrode by oxidative potentiodynamic polymerization. Rebinding and removal of the template were detected by cyclic voltammetry using ferricyanide as a redox marker. Furthermore, the enzymatic activity of BuChE rebound to the MIP was measured via the anodic oxidation of thiocholine, the reaction product of butyrylthiocholine. The response was linear between 50 pM and 2 nM concentrations of BuChE with a detection limit of 14.7 pM. In addition to the high sensitivity for BuChE, the sensor responded towards pseudo-irreversible inhibitors in the lower mM range.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 1138 
KW  - molecularly imprinted polymers
KW  - biomimetic sensors
KW  - butyrylcholinesterase
KW  - o-phenylenediamine
KW  - rivastigmine
Y1  - 2021
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-501854
SN  - 1866-8372
IS  - 1138
ER  - 
TY  - GEN
A1  - Yarman, Aysu
A1  - Scheller, Frieder W.
T1  - The first electrochemical MIP sensor for tamoxifen
T2  - Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe
N2  - We present an electrochemical MIP sensor for tamoxifen (TAM)-a nonsteroidal anti-estrogen-which is based on the electropolymerisation of an O-phenylenediamine. resorcinol mixture directly on the electrode surface in the presence of the template molecule. Up to now only. bulk. MIPs for TAM have been described in literature, which are applied for separation in chromatography columns. Electro-polymerisation of the monomers in the presence of TAM generated a film which completely suppressed the reduction of ferricyanide. Removal of the template gave a markedly increased ferricyanide signal, which was again suppressed after rebinding as expected for filling of the cavities by target binding. The decrease of the ferricyanide peak of the MIP electrode depended linearly on the TAM concentration between 1 and 100 nM. The TAM-imprinted electrode showed a 2.3 times higher recognition of the template molecule itself as compared to its metabolite 4-hydroxytamoxifen and no cross-reactivity with the anticancer drug doxorubucin was found. Measurements at + 1.1 V caused a fouling of the electrode surface, whilst pretreatment of TAM with peroxide in presence of HRP generated an oxidation product which was reducible at 0 mV, thus circumventing the polymer formation and electrochemical interferences.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 1046 
KW  - molecularly imprinted polymers
KW  - anticancer drug
KW  - tamoxifen
KW  - electropolymerisation
Y1  - 2020
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-476173
SN  - 1866-8372
IS  - 1046
ER  - 
TY  - JOUR
A1  - Bier, Frank Fabian
A1  - Kleinjung, Frank
A1  - Scheller, Frieder W.
T1  - Real time measurement of nucleic acid hybridization using evanescent wave sensors - step towards the genosensor
Y1  - 1997
ER  - 
TY  - JOUR
A1  - Ehrentreich-Förster, Eva
A1  - Scheller, Frieder W.
A1  - Bier, Frank Fabian
T1  - Detection of progesterone in whole blood samples
N2  - The progesterone concentration in blood samples can be utilised as a marker for the diagnosis of early pregnancy, endocrinopathy and virilism. Here, we describe a method for progesterone detection and measurement in whole blood samples by a surface sensitive biosensor used in conjunction with an integrated optical grating coupler. This device determines refractive index changes near the biosensor's surface. Hence, biological species bound to a surface layer can be measured in real-time without any label. For the measurements, we have modified the indirect competitive immonoassay principle. The concentration of the progesterone antibody was kept at 1 µg/ml. Progesterone concentration was determined in buffer solution and whole blood in a range between 0.005 and 10 ng/ml. The detection limit was determined to be 3 pM. The relative standard deviation was calculated to be 3.5%.
Y1  - 2003
ER  - 
TY  - JOUR
A1  - Scheller, Frieder W.
A1  - Bier, Frank Fabian
T1  - Biosensoren
Y1  - 2003
ER  - 
TY  - JOUR
A1  - Scheller, Frieder W.
A1  - Bier, Frank Fabian
T1  - Analytical Biochemistry (Editorial)
Y1  - 2004
ER  - 
TY  - JOUR
A1  - Scheller, Frieder W.
A1  - Bauer, Christian G.
A1  - Makower, Alexander
A1  - Wollenberger, Ursula
A1  - Warsinke, Axel
A1  - Bier, Frank Fabian
T1  - Immunoassays using enzymatic amplification electrodes
Y1  - 2002
SN  - 0-7484-0791-X
ER  - 
TY  - JOUR
A1  - Kleinjung, Frank
A1  - Klußmann, S.
A1  - Erdmann, V. A.
A1  - Scheller, Frieder W.
A1  - Fürste, J. P.
A1  - Bier, Frank Fabian
T1  - Novel binders in biosensorics : hight affinity RNA for smal analytes
Y1  - 1998
ER  - 
TY  - JOUR
A1  - Scheller, Frieder W.
A1  - Bauer, Christian G.
A1  - Markower, Alexander
A1  - Wollenberger, Ursula
A1  - Warsinke, Axel
A1  - Bier, Frank Fabian
T1  - Coupling of immunoassays with enzymatic recycling electrodes
Y1  - 2001
ER  - 
TY  - JOUR
A1  - Schmidt, Peter Michael
A1  - Matthes, E.
A1  - Scheller, Frieder W.
A1  - Bier, Frank Fabian
T1  - Nachweis der Telomeraseaktivität in Zellkulturen mittels eines faseroptischen Sensors
Y1  - 2001
ER  - 
TY  - BOOK
A1  - Wollenberger, Ursula
A1  - Renneberg, Reinhard
A1  - Bier, Frank Fabian
A1  - Scheller, Frieder W.
T1  - Analytische Biochemie : eine praktische Einführung in das Messen mit Biomolekülen
Y1  - 2003
SN  - 3-527-30166-6
PB  - John Wiley & Sons
CY  - Hoboken
ER  - 
TY  - JOUR
A1  - Scheller, Frieder W.
A1  - Bier, Frank Fabian
T1  - Trends in der Bioanalytik
Y1  - 2002
ER  - 
TY  - JOUR
A1  - Bier, Frank Fabian
A1  - Ehrentreich-Förster, Eva
A1  - Dölling, R.
A1  - Eremenko, A. V.
A1  - Scheller, Frieder W.
T1  - A redox-label immunosensor on basis of a bi-enzyme electrode
Y1  - 1997
ER  - 
TY  - JOUR
A1  - Makower, Alexander
A1  - Barmin, Anatoli V.
A1  - Morzunova, T.
A1  - Eremenko, Arkadi V.
A1  - Bier, Frank Fabian
A1  - Scheller, Frieder W.
T1  - Affinity enzymomoetric assay for detection of organophosphorus compounds
Y1  - 1997
ER  - 
TY  - JOUR
A1  - Bier, Frank Fabian
A1  - Ehrentreich-Förster, Eva
A1  - Scheller, Frieder W.
T1  - Amplifying bienzyme cycle-linked immunoassays for determination of 2,4- dichlorphenoxyacetic acid
Y1  - 1996
ER  - 
TY  - JOUR
A1  - Bier, Frank Fabian
A1  - Scheller, Frieder W.
T1  - Label-free observation of DNA-hybridisation and endonuclease activity on a wave guide surface using a grating coupler
Y1  - 1996
ER  - 
TY  - JOUR
A1  - Stöcklein, Walter F. M.
A1  - Makower, Alexander
A1  - Bier, Frank Fabian
A1  - Scheller, Frieder W.
T1  - Enzyme sensors and enzyme amplifification systems
Y1  - 1997
ER  - 
TY  - JOUR
A1  - Bier, Frank Fabian
A1  - Fürste, J. P.
A1  - Kleinjung, Frank
A1  - Erdmann, V. A.
A1  - Scheller, Frieder W.
T1  - Nukleinsäuren als Basis für Biosensoren
Y1  - 1997
ER  - 
TY  - JOUR
A1  - Scheller, Frieder W.
A1  - Bier, Frank Fabian
A1  - Gajovic, Nenad
T1  - Biosensoren und Teststreifen für die Umwelt- und Lebensmittelanalytik
Y1  - 1997
ER  - 
TY  - JOUR
A1  - Bier, Frank Fabian
A1  - Ehrentreich-Förster, Eva
A1  - Bauer, Christian G.
A1  - Scheller, Frieder W.
T1  - High sensitive competitive immunodetection of 2,4-dichlorophenoxyacetic acid using enzymatic amplification with electrochemical detection
Y1  - 1996
ER  -