TY  - JOUR
A1  - Scheller, Frieder W.
A1  - Schubert, Frank
A1  - Federowitz, J.
T1  - Present state and frontiers in biosensorics
Y1  - 1997
ER  - 
TY  - JOUR
A1  - Stöcklein, Walter F. M.
A1  - Scheller, Frieder W.
T1  - Enzymes and antibodies in organic media : analytical applications
Y1  - 1997
ER  - 
TY  - JOUR
A1  - Wollenberger, Ursula
A1  - Lisdat, Fred
A1  - Scheller, Frieder W.
T1  - Enzymatic substrade recycling electrodes
Y1  - 1997
ER  - 
TY  - JOUR
A1  - Eremenko, A. V.
A1  - Makower, Alexander
A1  - Bauer, Christian G.
A1  - Kurochkin, I. N.
A1  - Scheller, Frieder W.
T1  - A bienzyme electrode for tyrosine containing peptides determination
Y1  - 1997
ER  - 
TY  - JOUR
A1  - Xie, B.
A1  - Tang, X.
A1  - Wollenberger, Ursula
A1  - Johansson, G.
A1  - Gorton, Lo
A1  - Scheller, Frieder W.
A1  - Danielsson, B.
T1  - Hybrid biosensor for simultaneous electrochemical and thermal detection
Y1  - 1997
ER  - 
TY  - JOUR
A1  - Ehrentreich-Förster, Eva
A1  - Scheller, Frieder W.
A1  - McNeil, C. J.
T1  - Biosensor zur in vivo Messung von Superoxidradikalen
Y1  - 1997
ER  - 
TY  - JOUR
A1  - Szeponik, Jan
A1  - Möller, B.
A1  - Pfeiffer, Dorothea
A1  - Lisdat, Fred
A1  - Wollenberger, Ursula
A1  - Makower, Alexander
A1  - Scheller, Frieder W.
T1  - Ultrasensitive bienzyme sensor for adrenaline
Y1  - 1997
ER  - 
TY  - JOUR
A1  - Markower, Alexander
A1  - Wollenberger, Ursula
A1  - Hörtnagel, H.
A1  - Pfeiffer, Dorothea
A1  - Scheller, Frieder W.
T1  - Catecholamine detection using enzymatic amplification
Y1  - 1997
ER  - 
TY  - JOUR
A1  - Welzel, H.-P.
A1  - Kossmehl, G.
A1  - Engelmann, G.
A1  - Neumann, B.
A1  - Wollenberger, Ursula
A1  - Scheller, Frieder W.
T1  - Electrochemical polymerization of functionalized thiohene derivatives for immobilization of proteins
Y1  - 1997
ER  - 
TY  - JOUR
A1  - Gajovic, Nenad
A1  - Warsinke, Axel
A1  - Scheller, Frieder W.
T1  - Comparsion of two enzyme sequences for a novel L-malate biosensor
Y1  - 1997
ER  - 
TY  - JOUR
A1  - Katterle, Martin
A1  - Wollenberger, Ursula
A1  - Scheller, Frieder W.
T1  - Electrochemistry of hemoglobin at modified silver electrodes is not a redox-process of iron protoporhyrin IX
Y1  - 1997
ER  - 
TY  - JOUR
A1  - Ehrentreich-Förster, Eva
A1  - Scheller, Frieder W.
T1  - Charakterisierung antioxidativer Substanzen mit einem Superoxidsensor
Y1  - 1997
ER  - 
TY  - JOUR
A1  - Kulys, J.
A1  - Drungiliene, A.
A1  - Wollenberger, Ursula
A1  - Krikstopaitis, K.
A1  - Scheller, Frieder W.
T1  - Electroanalytical determination of peroxidases and laccases on carbon paste electrodes
Y1  - 1997
ER  - 
TY  - JOUR
A1  - Kleinjung, Frank
A1  - Beier, Frank F.
A1  - Warsinke, Axel
A1  - Scheller, Frieder W.
T1  - Fibre-optic genosensor for specific determination of femtomolar DNA oligomers
Y1  - 1997
ER  - 
TY  - JOUR
A1  - Pfeiffer, Dorothea
A1  - Yang, L.
A1  - Scheller, Frieder W.
A1  - Kissinger, P. T.
T1  - Continous measurement of lactate in microdialysate
Y1  - 1997
ER  - 
TY  - JOUR
A1  - Wollenberger, Ursula
A1  - Drungiliene, A.
A1  - Stöcklein, Walter F. M.
A1  - Kulys, J.
A1  - Scheller, Frieder W.
T1  - Direct electrocatalytic determination of dissolved peroxidases
Y1  - 1996
ER  - 
TY  - JOUR
A1  - Kaisheva, A.
A1  - Iliev, I.
A1  - Kazareva, R.
A1  - Christov, S.
A1  - Wollenberger, Ursula
A1  - Scheller, Frieder W.
T1  - Enzyme/gas diffusion electrodes for determination of phenol
Y1  - 1996
ER  - 
TY  - JOUR
A1  - Makower, Alexander
A1  - Eremenko, A. V.
A1  - Streffer, Katrin
A1  - Wollenberger, Ursula
A1  - Scheller, Frieder W.
T1  - Tyrosinase-glucose dehydrogenase substrate-recycling biosensor : a highly sensitive measurement of phenolic compounds
Y1  - 1996
ER  - 
TY  - JOUR
A1  - Spricigo, Roberto
A1  - Richter, Claudia
A1  - Leimkühler, Silke
A1  - Gorton, Lo
A1  - Scheller, Frieder W.
A1  - Wollenberger, Ursula
T1  - Sulfite biosensor based on osmium redox polymer wired sulfite oxidase
N2  - A biosensor, based on a redoxactive osmium polymer and sulfite oxidase on screen-printed electrodes, is presented here as a promising method for the detection of sulfite. A catalytic oxidative current was generated when a sample containing sulfite was pumped over the carbon screen-printed electrode modified with osmium redox polymer wired sulfite oxidase. A stationary value was reached after approximately 50 s and a complete measurement lasted no more than 3 min. The electrode polarized at -0.1 V (vs. Ag vertical bar AgCl 1M KCl) permits minimizing the influence of interfering substances, since these compounds can be unspecific oxidized at higher potentials. Because of the good stability of the protein film on the electrode surface, a well functioning biosensor-flow system was possible to construct. The working stability and reproducibility were further enhanced by the addition of bovine serum albumin generating a more long-term stable and biocompatible protein environment. The optimized biosensor showed a stable signal for more than a week of operation and a coefficient of variation of 4.8% for 12 successive measurements. The lower limit of detection of the sensor was 0.5 mu M sulfite and the response was linear until 100 mu M. The high sensitivity permitted a 1:500 dilution of wine samples. The immobilization procedure and the operational conditions granted minimized interferences. Additionally, repeating the immobilization procedure to form several layers of wired SO further increased the sensitivity of such a sensor. Finally. the applicability of the developed sulfite biosensor was tested on real samples, such as white and red wines.
Y1  - 2010
UR  - http://www.sciencedirect.com/science/journal/09277757
U6  - https://doi.org/10.1016/j.colsurfa.2009.09.001
SN  - 0927-7757
ER  - 
TY  - JOUR
A1  - Pfeiffer, Dorothea
A1  - Scheller, Frieder W.
A1  - McNeil, C. J.
A1  - Schulmeister, Thomas
T1  - Cascade-like exponential substrate amplification in enzyme sensors
Y1  - 1995
ER  - 
TY  - JOUR
A1  - Paeschke, Manfred
A1  - Hintsche, Rainer
A1  - Wollenberger, Ursula
A1  - Jin, Wen
A1  - Scheller, Frieder W.
T1  - Dynamic redox recycling of cytochrome c
Y1  - 1995
SN  - 0022-0728
ER  - 
TY  - JOUR
A1  - Scheller, Frieder W.
A1  - Pfeiffer, Dorothea
T1  - Biosensoren : ein wirtschaftlicher Faktor für die Zukunft
Y1  - 1994
ER  - 
TY  - JOUR
A1  - Scheller, Frieder W.
T1  - Biosensoren : Konzepte, Technologien, Perspektiven
Y1  - 1994
ER  - 
TY  - JOUR
A1  - Scheller, Frieder W.
A1  - Kirstein, Dieter
A1  - Pfeiffer, Dorothea
T1  - Biosensoren, Konzepte, Technologien, Perspektiven
Y1  - 1994
ER  - 
TY  - JOUR
A1  - Scheller, Frieder W.
A1  - Heiduschka, P.
T1  - Preparation of an electrode surface with a high density of binding sites by an electrochemical reduction of a poly (nitrophenol) film
Y1  - 1994
ER  - 
TY  - JOUR
A1  - Schmid, Rolf D.
A1  - Scheller, Frieder W.
T1  - Biosensoren
Y1  - 1994
ER  - 
TY  - JOUR
A1  - Lisdat, Fred
A1  - Dronov, Roman
A1  - Möhwald, Helmuth
A1  - Scheller, Frieder W.
A1  - Kurth, Dirk G.
T1  - Self-assembly of electro-active protein architectures on electrodes for the construction of biomimetic signal chains
N2  - The layer-by-layer adsorption technique based on the consecutive deposition of oppositely charged species is for the preparation of protein multilayers with fully electro-active protein molecules. The methodology was established with cytochrome c and the polyelectrolyte sulfonated polyaniline (PASA). The technique is also useful for the construction of bi-protein architectures confining protein-protein communication to an electrode. Following natural examples of protein complexes with defined signal transfer, cytochrome c was arranged with enzymes such as xanthine oxidase, bilirubin oxidase, laccase, and sulfite oxidase in self-assembled multilayer architectures. Thus, biomimetic signal chains from the enzyme substrate via the enzyme and cytochrome c towards the electrode can be established. Communication between proteins immobilised in multiple layers on the electrode can be achieved by in situ generation of small shuttle molecules or more advantageously by direct interprotein electron transfer. This allows the construction of new sensing electrodes, the properties of which can be tuned by the number of deposited protein layers. The mechanism of electron transfer within such protein assemblies on gold electrodes will be discussed.
Y1  - 2009
UR  - http://xlink.rsc.org/jumptojournal.cfm?journal_code=CC
U6  - https://doi.org/10.1039/B813559b
SN  - 1359-7345
ER  - 
TY  - JOUR
A1  - Loew, Noya
A1  - Wollenberger, Ursula
A1  - Scheller, Frieder W.
A1  - Katterle, Martin
T1  - Direct electrochemistry and spectroelectrochemistry of osmium substituted horseradish peroxidase
N2  - In this contribution the substitution of the central protoporphyrin IX iron complex of horseradish peroxidase by the respective osmium porphyrin complex is described. The direct electrochemical reduction of the Os containing horseradish peroxidase (OsHRP) was achieved at ITO and modified glassy carbon electrodes and in combination with spectroscopy revealed the three redox couples (OsHRP)-H-III/(OsHRP)-H-IV, (OsHRP)-H-IV/(OsHRP)-H-V and (OsHRP)-H-V/ (OsHRP)-H-VI. The midpoint potentials differ dependent on the electrode material used with E-1/2 (Os-III/IV) of -0.4 V (ITO) and -0.25 V (GC), E-1/2 (Os-IV/V) of -0.16 V (ITO) and +0.10 V (GC), and E-1/2 (Os-V/VI)of +018 V (ITO), respectively Moreover, with immobilised OsHRP the direct electrocatalytic reduction of hydrogen peroxide and tert-butyl hydroperoxide was observed. In comparison to electrodes modified with native HRP the sensitivity of the OsHRP-electrode for tert-butyl hydroperoxide is higher.
Y1  - 2009
UR  - http://www.sciencedirect.com/science/journal/15675394
U6  - https://doi.org/10.1016/j.bioelechem.2009.03.015
SN  - 1567-5394
ER  - 
TY  - JOUR
A1  - Schulmeister, Thomas
A1  - Scheller, Frieder W.
T1  - The mathematics of exponential signal amplification in amperometric three enzyme electrodes
Y1  - 1996
ER  - 
TY  - JOUR
A1  - Stancik, L.
A1  - Macholán, L.
A1  - Scheller, Frieder W.
T1  - Biosensing of tyrosinase inhibitors in nonaqueous solvents
Y1  - 1995
ER  - 
TY  - JOUR
A1  - Stöcklein, Walter F. M.
A1  - Scheller, Frieder W.
A1  - Abuknesha, Rhamadan
T1  - Effects of organic solvents on semicontinuous immunochemical detection of coumarin derivatives
Y1  - 1995
ER  - 
TY  - JOUR
A1  - Stöcklein, Walter F. M.
A1  - Scheller, Frieder W.
T1  - Einsatz von Enzymen und Antikörpern in organischen Lösungsmitteln
Y1  - 1995
ER  - 
TY  - JOUR
A1  - Wollenberger, Ursula
A1  - Hintsche, R.
A1  - Scheller, Frieder W.
T1  - Biosensors for analytical microsystems
Y1  - 1995
ER  - 
TY  - JOUR
A1  - Eremenko, A. V.
A1  - Makower, Alexander
A1  - Scheller, Frieder W.
T1  - Measurement of nanomolar diphenols by substrate recycling coupled to a pH- sensitive electrode
Y1  - 1995
ER  - 
TY  - JOUR
A1  - Gajovic, Nenad
A1  - Warsinke, Axel
A1  - Scheller, Frieder W.
T1  - A novel multienzyme electrode for the determination of citrate
Y1  - 1995
ER  - 
TY  - JOUR
A1  - Ghindilis, A. L.
A1  - Makower, Alexander
A1  - Scheller, Frieder W.
T1  - Laccase - glucose dehydrogenase recycling enzyme electrode based on potentiometric mediatorless electrocatalytic detection
Y1  - 1995
ER  - 
TY  - JOUR
A1  - Ghindilis, A. L.
A1  - Makower, Alexander
A1  - Scheller, Frieder W.
T1  - Nanomolar determination of the ferrocene derivatives using a recycling enzyme electrode : development of the redox label immunoassay
Y1  - 1995
ER  - 
TY  - JOUR
A1  - Ghindilis, A. L.
A1  - Makower, Alexander
A1  - Scheller, Frieder W.
T1  - Potentiometric enzyme electrodes based on substrate recycling and mediatorless bioelectrocatalysis
Y1  - 1995
ER  - 
TY  - JOUR
A1  - Lisdat, Fred
A1  - Wollenberger, Ursula
A1  - Paeschke, Manfred
A1  - Scheller, Frieder W.
T1  - Sensitive catecholamine measurement using a monoenzymatic recycling system
Y1  - 1998
ER  - 
TY  - JOUR
A1  - Stancík, L.
A1  - Macholán, L.
A1  - Pluhacek, I.
A1  - Scheller, Frieder W.
T1  - Biosensing of rapeseed glucosinolates using amperometric enzyme electrodes based on membrane-bound glucose oxidase or tyrosinase
Y1  - 1995
ER  - 
TY  - JOUR
A1  - Eremenko, A. V.
A1  - Makower, Alexander
A1  - Jin, Wen
A1  - Rüger, P.
A1  - Scheller, Frieder W.
T1  - Biosensor based on an enzyme modified electrode for highly - sensitive measurement of polyphenols
Y1  - 1995
ER  - 
TY  - JOUR
A1  - Iliev, I.
A1  - Kaisheva, A.
A1  - Scheller, Frieder W.
A1  - Pfeiffer, Dorothea
T1  - Amperometric gas-diffusion / enzyme electrode
Y1  - 1995
ER  - 
TY  - JOUR
A1  - Riedel, K.
A1  - Beyersdorf-Radeck, Baerbel
A1  - Neumann, B.
A1  - Scheller, Frieder W.
A1  - Schmid, Rolf D.
T1  - Microbial sensors for determination of aromatics and their chloro derivatives. Part III: Determination of chlorinated phenols using a biosensor containing Trichosporon beigelii (cutaneum)
Y1  - 1995
ER  - 
TY  - JOUR
A1  - Shumyantseva, V. V.
A1  - Ivanov, Y. D.
A1  - Bistolas, Nikitas
A1  - Scheller, Frieder W.
A1  - Archakov, Alexander I.
A1  - Wollenberger, Ursula
T1  - Direct electron transfer of cytochrome P450 2B4 at electrodes modified with non-ionic detergent and colloidal clay nanoparticles
N2  - A method for construction of biosensors with membranous cytochrome P450 isoenzymes was developed based on clay/ detergent/protein mixed films. Thin films of sodium montmorillonite colloid with incorporated cytochrome P450 2134 (CYP2B4) with nonionic detergent were prepared on glassy carbon electrodes. The modified electrodes were electrochemically characterized, and bio-electrocatalytic reactions were followed. CYP2B4 can be reduced fast on clay- modified glassy carbon electrodes in the presence of the nonionic detergent Tween 80. In anaerobic solutions, reversible oxidation and reduction is obtained with a formal potential between -0.292 and - 0.305 V vs Ag/AgCl 1 M KCl depending on the preparation of the biosensor. In air-saturated solution, bio-electrocatalytic reduction currents can be obtained with the CYP2B4-modified electrode on addition of typical substrates such as aminopyrine and benzphetamine. This reaction was suppressed when methyrapone, an inhibitor of P450 reactions, was present. Measurement of product formation also indicates the bioelectrocatialysis by CYP2B4
Y1  - 2004
ER  - 
TY  - JOUR
A1  - Frasca, Stefano
A1  - von Graberg, Till
A1  - Feng, Jiu-Ju
A1  - Thomas, Arne
A1  - Smarsly, Bernd M.
A1  - Weidinger, Inez M.
A1  - Scheller, Frieder W.
A1  - Hildebrandt, Peter
A1  - Wollenberger, Ursula
T1  - Mesoporous indium tin oxide as a novel platform for bioelectronics
N2  - Stable immobilization and reversible electrochemistry of cytochrome c in a tranparent indium tin oxide film with a well-defined mesoporosity (mpITO) is demonstrated. the transparency and good conductivity, in combination with the large surface area of mpITO, allow the incorporation of a high amount of elelctroactive biomolecules and their electrochemical and spectroscopic investigation. UV/Vis and resonance Raman spectroscopy, in combination with direct protein voltammetry are employed for the characterization of cytochrome c immobilized in the mpITO and reveal no perturbant of the structural of the integrity of the redox protein. The potential of this modified material as a biosensor detection of superoxide anions is also demonstrated.
Y1  - 2010
UR  - http://www3.interscience.wiley.com/journal/122208635/home
U6  - https://doi.org/10.1002/cctc.201000047
SN  - 1867-3880
ER  - 
TY  - JOUR
A1  - Stöcklein, Walter F. M.
A1  - Warsinke, Axel
A1  - Micheel, Burkhard
A1  - Höhne, Wolfgang
A1  - Woller, Jochen
A1  - Kempter, Gerhard
A1  - Scheller, Frieder W.
T1  - Characterization of a monoclonal antibody and its Fab fragment against diphenylurea hapten with BIA
Y1  - 1998
SN  - 3-8154-3540-4
ER  - 
TY  - JOUR
A1  - Warsinke, Axel
A1  - Stancik, L.
A1  - Macholán, L.
A1  - Pfeiffer, Dorothea
A1  - Scheller, Frieder W.
T1  - Biosensors for food analysis : application of biosensors to food requirements
Y1  - 1998
SN  - 0-85404-750-6
ER  - 
TY  - JOUR
A1  - Stöcklein, Walter F. M.
A1  - Warsinke, Axel
A1  - Micheel, Burkhard
A1  - Höhne, Wolfgang
A1  - Woller, Jochen
A1  - Kempter, Gerhard
A1  - Scheller, Frieder W.
T1  - Detection of diphenylurea derivatives with biospecific interaction analysis (BIA) : Kinetic investigations
Y1  - 1997
ER  - 
TY  - JOUR
A1  - Spricigo, Roberto
A1  - Dronov, Roman
A1  - Lisdat, Fred
A1  - Leimkühler, Silke
A1  - Scheller, Frieder W.
A1  - Wollenberger, Ursula
T1  - Electrocatalytic sulfite biosensor with human sulfite oxidase co-immobilized with cytochrome c in a polyelectrolyte-containing multilayer
N2  - An efficient electrocatalytic biosensor for sulfite detection was developed by co-immobilizing sulfite oxidase and cytochrome c with polyaniline sulfonic acid in a layer-by-layer assembly. QCM, UV-Vis spectroscopy and cyclic voltammetry revealed increasing loading of electrochemically active protein with the formation of multilayers. The sensor operates reagentless at low working potential. A catalytic oxidation current was detected in the presence of sulfite at the modified gold electrode, polarized at +0.1 V ( vs. Ag/AgCl 1 M KCl). The stability of the biosensor performance was characterized and optimized. A 17-bilayer electrode has a linear range between 1 and 60 mu M sulfite with a sensitivity of 2.19 mA M-1 sulfite and a response time of 2 min. The electrode retained a stable response for 3 days with a serial reproducibility of 3.8% and lost 20% of sensitivity after 5 days of operation. It is possible to store the sensor in a dry state for more than 2 months. The multilayer electrode was used for determination of sulfite in unspiked and spiked samples of red and white wine. The recovery and the specificity of the signals were evaluated for each sample.
Y1  - 2009
UR  - http://www.springerlink.com/content/100417
U6  - https://doi.org/10.1007/s00216-008-2432-y
SN  - 1618-2642
ER  - 
TY  - JOUR
A1  - Ehrentreich-Förster, Eva
A1  - Shishniashvili, D.
A1  - Song, Min Ik
A1  - Scheller, Frieder W.
T1  - Study of antioxidative substances by means of a ssuperoxide sensor
Y1  - 1998
ER  -