TY  - GEN
A1  - Abramowski, Attila
A1  - Aharonian, Felix A.
A1  - Benkhali, Faical Ait
A1  - Akhperjanian, A. G.
A1  - Angüner, Ekrem Oǧuzhan
A1  - Backes, Michael
A1  - Balenderan, Shangkari
A1  - Balzer, Arnim
A1  - Barnacka, Anna
A1  - Becherini, Yvonne
A1  - Tjus, Julia Becker
A1  - Berge, David
A1  - Bernhard, Sabrina
A1  - Bernlöhr, Konrad
A1  - Birsin, E.
A1  - Biteau, Jonathan
A1  - Böttcher, Markus
A1  - Boisson, Catherine
A1  - Bolmont, J.
A1  - Bordas, Pol
A1  - Bregeon, Johan
A1  - Brun, Francois
A1  - Brun, Pierre
A1  - Bryan, Mark
A1  - Bulik, Tomasz
A1  - Carrigan, Svenja
A1  - Casanova, Sabrina
A1  - Chadwick, Paula M.
A1  - Chakraborty, Nachiketa
A1  - Chalme-Calvet, R.
A1  - Chaves, Ryan C. G.
A1  - Chretien, M.
A1  - Colafrancesco, Sergio
A1  - Cologna, Gabriele
A1  - Conrad, Jan
A1  - Couturier, Claire
A1  - Cui, Yudong
A1  - Davids, Isak Delberth
A1  - Degrange, Bernhard
A1  - Deil, Christoph
A1  - deWilt, P.
A1  - Djannati-Ataï, A.
A1  - Domainko, Wilfried
A1  - Donath, Axel
A1  - Dubus, G.
A1  - Dutson, K.
A1  - Dyks, J.
A1  - Dyrda, M.
A1  - Edwards, Tanya
A1  - Egberts, Kathrin
A1  - Eger, Peter
A1  - Espigat, P.
A1  - Farnier, C.
A1  - Fegan, Stephen
A1  - Feinstein, Fabrice
A1  - Fernandes, Milton Virgilio
A1  - Fernandez, Diane
A1  - Fiasson, A.
A1  - Fontaine, Gerard
A1  - Förster, Andreas
A1  - Fuessling, M.
A1  - Gabici, S.
A1  - Gajdus, M.
A1  - Gallant, Yves A.
A1  - Garrigoux, Tania
A1  - Giavitto, G.
A1  - Giebels, Berrie
A1  - Glicenstein, Jean-Francois
A1  - Gottschall, Daniel
A1  - Grondin, M. -H.
A1  - Grudzinska, M.
A1  - Hadasch, Daniela
A1  - Haeffner, S.
A1  - Hahn, Joachim
A1  - Harris, Jonathan
A1  - Heinzelmann, Götz
A1  - Henri, G.
A1  - Hermann, German
A1  - Hervet, O.
A1  - Hillert, Andreas
A1  - Hinton, James Anthony
A1  - Hofmann, Werner
A1  - Hofverberg, Petter
A1  - Holler, Markus
A1  - Horns, Dieter
A1  - Ivascenko, Alex
A1  - Jacholkowska, A.
A1  - Jahn, C.
A1  - Jamrozy, Marek
A1  - Janiak, M.
A1  - Jankowsky, F.
A1  - Jung-Richardt, I.
A1  - Kastendieck, Max Anton
A1  - Katarzynski, K.
A1  - Katz, U.
A1  - Kaufmann, S.
A1  - Khelifi, B.
A1  - Kieffer, Michel
A1  - Klepser, S.
A1  - Klochkov, Dmitry
A1  - Kluzniak, W.
A1  - Kolitzus, David
A1  - Komin, Nu
A1  - Kosack, Karl
A1  - Krakau, Steffen
A1  - Krayzel, F.
A1  - Krueger, Pat P.
A1  - Laffon, H.
A1  - Lamanna, G.
A1  - Lefaucheur, J.
A1  - Lefranc, Valentin
A1  - Lemiere, A.
A1  - Lemoine-Goumard, M.
A1  - Lenain, J. -P.
A1  - Lohse, Thomas
A1  - Lopatin, A.
A1  - Lu, Chia-Chun
A1  - Marandon, Vincent
A1  - Marcowith, Alexandre
A1  - Marx, Ramin
A1  - Maurin, G.
A1  - Maxted, Nigel
A1  - Mayer, Michael
A1  - McComb, T. J. Lowry
A1  - Mehault, J.
A1  - Meintjes, P. J.
A1  - Menzler, Ulf
A1  - Meyer, M.
A1  - Mitchell, Alison M. W.
A1  - Moderski, R.
A1  - Mohamed, M.
A1  - Mora, K.
A1  - Moulin, Emmanuel
A1  - Murach, Thomas
A1  - de Naurois, Mathieu
A1  - Niemiec, J.
A1  - Nolan, Sam J.
A1  - Oakes, Louise
A1  - Odaka, Hirokazu
A1  - Ohm, S.
A1  - Optiz, Björn
A1  - Ostrowski, Michal
A1  - Oya, I.
A1  - Panter, Michael
A1  - Parsons, R. Daniel
A1  - Arribas, M. Paz
A1  - Pekeur, Nikki W.
A1  - Pelletier, G.
A1  - Petrucci, P. -O.
A1  - Peyaud, B.
A1  - Pita, S.
A1  - Poon, Helen
A1  - Pühlhofer, Gerd
A1  - Punch, M.
A1  - Quirrenbach, A.
A1  - Raab, S.
A1  - Reichardt, I.
A1  - Reimer, Anita
A1  - Reimer, Olaf
A1  - Renaud, Metz
A1  - de los Reyes, Raquel
A1  - Rieger, Frank
A1  - Romoli, C.
A1  - Rosier-Lees, S.
A1  - Rowell, G.
A1  - Rudak, B.
A1  - Rulten, C. B.
A1  - Sahakian, Vardan
A1  - Salek, D.
A1  - Sanchez, David M.
A1  - Santangelo, Andrea
A1  - Schlickeiser, Reinhard
A1  - Schuessler, F.
A1  - Schulz, A.
A1  - Schwanke, Ullrich
A1  - Schwarzburg, S.
A1  - Schwemmer, S.
A1  - Sol, H.
A1  - Spanier, Felix
A1  - Spengler, G.
A1  - Spies, Franziska
A1  - Stawarz, Lukasz
A1  - Steenkamp, Riaan
A1  - Stegmann, Christian
A1  - Stinzing, F.
A1  - Stycz, K.
A1  - Sushch, Iurii
A1  - Tavernet, J. -P.
A1  - Tavernier, T.
A1  - Taylor, A. M.
A1  - Terrier, R.
A1  - Tluczykont, Martin
A1  - Trichard, C.
A1  - Valerius, K.
A1  - van Eldik, C.
A1  - van Soelen, B.
A1  - Vasileiadis, Georges
A1  - Veh, J.
A1  - Venter, Christo
A1  - Viana, Aion
A1  - Vincent, P.
A1  - Vink, Jacco
A1  - Völk, Heinrich J.
A1  - Volpe, Francesca
A1  - Vorster, Martine
A1  - Vuillaume, T.
A1  - Wagner, S. J.
A1  - Wagner, P.
A1  - Wagner, R. M.
A1  - Ward, Martin
A1  - Weidinger, Matthias
A1  - Weitzel, Quirin
A1  - White, R.
A1  - Wierzcholska, A.
A1  - Willmann, P.
A1  - Woernlein, A.
A1  - Wouters, D.
A1  - Yang, Ruizhi
A1  - Zabalza, Victor
A1  - Zaborov, Dmitry
A1  - Zacharias, M.
A1  - Zdziarski, A. A.
A1  - Zech, Alraune
A1  - Zechlin, Hannes -S.
T1  - H.E.S.S. detection of TeV emission from the interaction region between the supernova remnant G349.7+0.2 and a molecular cloud (vol 574, A100, 2015)
T2  - Astronomy and astrophysics : an international weekly journal
KW  - gamma rays: general
KW  - ISM: supernova remnants
KW  - ISM: clouds
KW  - errata, addenda
Y1  - 2015
U6  - https://doi.org/10.1051/0004-6361/201425070e
SN  - 1432-0746
VL  - 580
PB  - EDP Sciences
CY  - Les Ulis
ER  - 
TY  - JOUR
A1  - Tscheuschner, Georg
A1  - Kaiser, Melanie N.
A1  - Lisec, Jan
A1  - Beslic, Denis
A1  - Muth, Thilo
A1  - Krüger, Maren
A1  - Mages, Hans Werner
A1  - Dorner, Brigitte G.
A1  - Knospe, Julia
A1  - Schenk, Jörg A.
A1  - Sellrie, Frank
A1  - Weller, Michael G.
T1  - MALDI-TOF-MS-based identification of monoclonal murine Anti-SARS-CoV-2 antibodies within one hour
JF  - Antibodies
N2  - During the SARS-CoV-2 pandemic, many virus-binding monoclonal antibodies have been developed for clinical and diagnostic purposes. This underlines the importance of antibodies as universal bioanalytical reagents. However, little attention is given to the reproducibility crisis that scientific studies are still facing to date. In a recent study, not even half of all research antibodies mentioned in publications could be identified at all. This should spark more efforts in the search for practical solutions for the traceability of antibodies. For this purpose, we used 35 monoclonal antibodies against SARS-CoV-2 to demonstrate how sequence-independent antibody identification can be achieved by simple means applied to the protein. First, we examined the intact and light chain masses of the antibodies relative to the reference material NIST-mAb 8671. Already half of the antibodies could be identified based solely on these two parameters. In addition, we developed two complementary peptide mass fingerprinting methods with MALDI-TOF-MS that can be performed in 60 min and had a combined sequence coverage of over 80%. One method is based on the partial acidic hydrolysis of the protein by 5 mM of sulfuric acid at 99 degrees C. Furthermore, we established a fast way for a tryptic digest without an alkylation step. We were able to show that the distinction of clones is possible simply by a brief visual comparison of the mass spectra. In this work, two clones originating from the same immunization gave the same fingerprints. Later, a hybridoma sequencing confirmed the sequence identity of these sister clones. In order to automate the spectral comparison for larger libraries of antibodies, we developed the online software ABID 2.0. This open-source software determines the number of matching peptides in the fingerprint spectra. We propose that publications and other documents critically relying on monoclonal antibodies with unknown amino acid sequences should include at least one antibody fingerprint. By fingerprinting an antibody in question, its identity can be confirmed by comparison with a library spectrum at any time and context.
KW  - SARS-CoV-2 antibody
KW  - reproducibility crisis
KW  - peptide mass
KW  - fingerprinting
KW  - monoclonal antibody
KW  - traceability
KW  - identity
KW  - antibody
KW  - identification
KW  - antibody light chain
KW  - MALDI-TOF-MS
Y1  - 2022
U6  - https://doi.org/10.3390/antib11020027
SN  - 2073-4468
VL  - 11
IS  - 2
PB  - MDPI
CY  - Basel
ER  -