TY - JOUR A1 - Auyyuenyong, Ratchada A1 - Henze, Andrea A1 - Ungru, Julia A1 - Schweigert, Florian Johannes A1 - Raila, Jens A1 - Vervuert, Ingrid T1 - Determination of lipid profiles in serum of obese ponies before and after weight reduction by using multi-one-dimensional thin-layer chromatography JF - Research in veterinary science N2 - Obesity is a key component of equine metabolic syndrome, which is highly associated with laminitis. Feed restriction and/or exercise are known to alleviate the detrimental effects of insulin resistance in obese ponies. However, little is known about changes in the serum lipid patterns due to weight reduction and its association with disease outcomes. Therefore, the lipid patterns in the serum of 14 mature ponies before and after a 14-week body weight reduction program (BWRP) were investigated by multi-one-dimensional thin-layer chromatography (MOD-TLC). Additionally, sensitivity to insulin (SI), body condition scores (BCS) and cresty neck scores (CNS) were measured. A BWRP resulted in a significant loss of body weight (P < 0.001), which was associated with beneficial decreases in BCS and CNS (both, P < 0.001). Serum lipid compositions revealed significantly increased free fatty acid (FFA), sphingomyelin (SM; both P < 0.001), total cholesterol (C) and cholesterol ester (CE) (both P < 0.01) and triacylglycerol (TG; P < 0.05) densities. Improvement of SI after the BWRP was associated with increases in neutral lipids (C, CE and TG, all P < 0.01), FFA and the phospholipid SM (both, P < 0.001). The results show that a BWRP in obese ponies was effective and associated with changes in the concentrations of neutral lipids and the phospholipid SM, indicating that SM may play a role in insulin signaling pathways and thus in the pathogenesis of insulin resistance and the progression of metabolic syndrome in obese ponies. KW - Neutral lipids KW - Equine metabolic syndrome KW - Phospholipids KW - Horse KW - Thin layer chromatography Y1 - 2017 U6 - https://doi.org/10.1016/j.rvsc.2017.11.013 SN - 0034-5288 SN - 1532-2661 VL - 117 SP - 111 EP - 117 PB - Elsevier CY - Oxford ER - TY - JOUR A1 - Fruscalzo, Arrigo A1 - Frommer, Julia-Marie A1 - Londero, Ambrogio P. A1 - Henze, Andrea A1 - Schweigert, Florian J. A1 - Nofer, Jerzy-Roch A1 - Steinhard, Johannes A1 - Klockenbusch, Walter A1 - Schmitz, Ralf A1 - Raila, Jens T1 - First trimester TTR-RBP4-ROH complex and angiogenic factors in the prediction of small for gestational age infant’s outcome JF - Archives of gynecology and obstetrics N2 - To study the role of the TTR-RBP4-ROH complex components (transthyretin, serum retinol binding protein, retinol) and of angiogenic factors PlGF (placental growth factor) and sFlt-1 (soluble fms-like tyrosine kinase-1) in pregnancies complicated by small for gestational age infants (SGA). Case control study conducted on maternal serum collected between 11 + 0 to 13 + 6 weeks of gestation. TTR, RBP4, ROH, PlGF and sFlt-1 were measured in SGA patients (birth weight < 10%) who delivered at term (n = 37) and before 37 weeks of gestation (n = 17) and in a matched control group with uneventful pregnancies (n = 37). We found decreased RBP4 in SGA patients that delivered fetuses < 3% and in fetuses delivered after the 37 weeks of gestation compared to controls [1.50 (95% CI 1.40-1.75) vs 1.62 (95% CI 1.47-1.98), p < 0.05]. Further, we found lower PlGF and sFlt-1 concentrations in SGA that delivered before 37 weeks of gestation compared to controls (respectively, PIGF and sFlt-1: 39.7 pg/ml (95% CI 32.3-66.3) vs 62.9 pg/ml (95% CI 45.2-78.4) and 906 pg/ml (95% CI 727-1626) vs 1610 pg/ml (95% CI 1088-212), p < 0.05). First trimester maternal serum RBP4 and angiogenic factors PlGF and sFlt-1 can differently predict the timing of delivery of pregnancies complicated by SGA fetuses. KW - Low birth weight KW - Small for gestational age KW - Pregnancy KW - First trimester KW - Marker KW - RBP4 KW - TTR KW - Retinol KW - Vitamin A KW - sFlt-1 KW - PlGF Y1 - 2017 U6 - https://doi.org/10.1007/s00404-017-4338-4 SN - 0932-0067 SN - 1432-0711 VL - 295 SP - 1157 EP - 1165 PB - Springer CY - Heidelberg ER - TY - JOUR A1 - Gebhardt, Constance A1 - Hirschberger, Johannes A1 - Rau, Stefanie A1 - Arndt, Gisela A1 - Krainer, Karen A1 - Schweigert, Florian J. A1 - Brunnberg, Leo A1 - Kaspers, Bernd A1 - Kohn, Barbara T1 - Use of C-reactive protein to predict outcome in dogs with systemic inflammatory response syndrome or sepsis N2 - Background There is a high mortality rate in patients with systemic inflammatory response syndrome (SIRS) or sepsis. Therefore, an early diagnosis and prognostic assessment is important for optimal therapeutic intervention. The objective of the study was to evaluate if baseline values and changes in serum C-reactive protein (CRP) might predict survival in dogs with SIRS and sepsis. Design Prospective study; July 2004 to July 2005. Setting Small Animal Clinic, Berlin, Clinic of Small Animal Medicine, Munich. Animals Sixty-one dogs. Measurements and Main Results For the CRP analysis blood was drawn on day 0, 1, and 2; CRP was measured using a commercial ELISA test kit. Thirteen dogs suffered from nonseptic SIRS and 48 dogs from sepsis. The 14-day survival rate was 61% (69% nonseptic SIRS, 58% sepsis). Serum CRP was higher in sick dogs compared with controls (P < 0.001). Over the 3-day period surviving dogs (n=31) displayed a significantly greater decrease in CRP than nonsurvivors (n=10) (P=0.001). No correlation was found between the initial CRP concentrations and the survival rate. The changes in CRP corresponded to the survival rate (P=0.01). Conclusion There was no significant relationship between the survival rate in dogs with nonseptic SIRS or sepsis and the initial serum CRP concentrations. There was a correlation between decreasing CRP concentrations and recovery from disease. However, the changes in CRP concentrations over a 3-day period correctly predicted survival in 94% of dogs and death in 30% of the dogs (false positive rate 22%). Y1 - 2009 UR - http://www3.interscience.wiley.com/cgi-bin/issn?DESCRIPTOR=PRINTISSN&VALUE=1479-3261 U6 - https://doi.org/10.1111/j.1476-4431.2009.00462.x SN - 1479-3261 ER -