TY  - THES
A1  - Sokolowska, Ewelina Maria
T1  - Implementation of a plasmodesmata gatekeeper system, and its effect on intercellular transport
Y1  - 2016
ER  - 
TY  - JOUR
A1  - Yang, Lei
A1  - Perrera, Valentina
A1  - Saplaoura, Eleftheria
A1  - Apelt, Federico
A1  - Bahin, Mathieu
A1  - Kramdi, Amira
A1  - Olas, Justyna Jadwiga
A1  - Müller-Röber, Bernd
A1  - Sokolowska, Ewelina
A1  - Zhang, Wenna
A1  - Li, Runsheng
A1  - Pitzalis, Nicolas
A1  - Heinlein, Manfred
A1  - Zhang, Shoudong
A1  - Genovesio, Auguste
A1  - Colot, Vincent
A1  - Kragler, Friedrich
T1  - m(5)C Methylation Guides Systemic Transport of Messenger RNA over Graft Junctions in Plants
JF  - Current biology
N2  - In plants, transcripts move to distant body parts to potentially act as systemic signals regulating development and growth. Thousands of messenger RNAs (mRNAs) are transported across graft junctions via the phloem to distinct plant parts. Little is known regarding features, structural motifs, and potential base modifications of transported transcripts and how these may affect their mobility. We identified Arabidopsis thalianam RNAs harboring the modified base 5-methylcytosine (m(5)C) and found that these are significantly enriched in mRNAs previously described as mobile, moving over graft junctions to distinct plant parts. We confirm this finding with graft-mobile methylated mRNAs TRANSLATIONALLY CONTROLLED TUMOR PROTEIN 1 (TCTP1) and HEAT SHOCK COGNATE PROTEIN 70.1 (HSC70.1), whose mRNA transport is diminished in mutants deficient in m(5)C mRNA methylation. Together, our results point toward an essential role of cytosine methylation in systemic mRNA mobility in plants and that TCTP1 mRNA mobility is required for its signaling function.
Y1  - 2019
U6  - https://doi.org/10.1016/j.cub.2019.06.042
SN  - 0960-9822
SN  - 1879-0445
VL  - 29
IS  - 15
SP  - 2465
EP  - 2476.e5
PB  - Cell Press
CY  - Cambridge
ER  - 
TY  - JOUR
A1  - Friedrich, Thomas
A1  - Oberkofler, Vicky
A1  - Trindade, Inês
A1  - Altmann, Simone
A1  - Brzezinka, Krzysztof
A1  - Lämke, Jörn S.
A1  - Gorka, Michal
A1  - Kappel, Christian
A1  - Sokolowska, Ewelina
A1  - Skirycz, Aleksandra
A1  - Graf, Alexander
A1  - Bäurle, Isabel
T1  - Heteromeric HSFA2/HSFA3 complexes drive transcriptional memory after heat stress in Arabidopsis
JF  - Nature Communications
N2  - Adaptive plasticity in stress responses is a key element of plant survival strategies. For instance, moderate heat stress (HS) primes a plant to acquire thermotolerance, which allows subsequent survival of more severe HS conditions. Acquired thermotolerance is actively maintained over several days (HS memory) and involves the sustained induction of memory-related genes. Here we show that FORGETTER3/ HEAT SHOCK TRANSCRIPTION FACTOR A3 (FGT3/HSFA3) is specifically required for physiological HS memory and maintaining high memory-gene expression during the days following a HS exposure. HSFA3 mediates HS memory by direct transcriptional activation of memory-related genes after return to normal growth temperatures. HSFA3 binds HSFA2, and in vivo both proteins form heteromeric complexes with additional HSFs. Our results indicate that only complexes containing both HSFA2 and HSFA3 efficiently promote transcriptional memory by positively influencing histone H3 lysine 4 (H3K4) hyper-methylation. In summary, our work defines the major HSF complex controlling transcriptional memory and elucidates the in vivo dynamics of HSF complexes during somatic stress memory. Moderate heat stress primes plants to acquire tolerance to subsequent, more severe heat stress. Here the authors show that the HSFA3 transcription factor forms a heteromeric complex with HSFA2 to sustain activated transcription of genes required for acquired thermotolerance by promoting H3K4 hyper-methylation.
Y1  - 2021
U6  - https://doi.org/10.1038/s41467-021-23786-6
SN  - 2041-1723
VL  - 12
IS  - 1
PB  - Nature Publishing Group UK
CY  - [London]
ER  -