TY  - THES
A1  - Schwarz, Doreen
T1  - Identifikationsmuster und Bindungsorientierungen von frei- und nebenberuflich Lehrenden in Volkshochschulen : eine qualitative Untersuchung
T1  - Pattern of identification and bonding orientations of freelance and part-time lectures in adult education centre : a qualitative research
N2  - Frei- und nebenberuflich Lehrende bilden die zahlenmäßig stärkste ‚Berufsgruppe‘ an Volkshochschulen und dennoch: Im Rahmen von Professionalität, Organisations- und Qualitätsentwicklung finden sie bis heute als eigenständige Personengruppe nur wenig Beachtung. In der Wissenschaft sind es zumeist quantitative Studien, in denen die soziale Lage der Lehrenden, ihr pädagogisches Handeln sowie ihre Organisationsge- und verbundenheit thematisiert werden. Studien, welche die Subjektperspektive in diesem Kontext gezielt in den Mittelpunkt stellen, gibt es dagegen nicht. Vor diesem Hintergrund geht es in der vorliegenden qualitativen Untersuchung explizit um die Gruppe der frei- und nebenberuflich Lehrenden. Hinterfragt wird, auf welche Weise sich nicht fest angestellte Mitarbeiter/innen mit der Organisation, für die sie arbeiten sowie mit ihrer dortigen Tätigkeit identifizieren können und wie sie sich darin begründen. Im Ergebnis werden zwei kontrastreiche Identifikationsmuster und Begründungstypen vorgestellt, die das Verhältnis der Lehrenden zu ‚ihren‘ Organisationen veranschaulichen und die erkennen lassen, welche Konsequenzen sich daraus für die Volkshochschule im Allgemeinen ergeben.
N2  - Freelance and part-time lecturers constitute the numeral biggest ‘occupational category’ in adult education centre. Nevertheless: They do not find highly attention in the context of professionalism, organizational and quality development. In science exist quantitative studies mostly, which broach the issue of social circumstances of lectures, their educational actions as well as their organizational bondage and solidarity. There are no studies, which make a point of spotlighting the subject perspective. Against this background the available qualitative research paper explicit concentrate on the group of freelance and part-time lectures. The question is, in which manner non salaried employees are able to identify with the organization they work for as well as their local practice and how they reason it. As a result two high contrasts pattern of identification and types of grounds will be presented, which visualize the relationship between lecturers and ‘their’ organizations and which show the consequences for adult education centre in general.
KW  - frei- und nebenberuflich Lehrende
KW  - Volkshochschule
KW  - Professionalität
KW  - Organisation
KW  - Qualitätsentwicklung
KW  - freelance and part-time lecturers
KW  - adult education centre
KW  - professionalism
KW  - organization
KW  - quality development
Y1  - 2008
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-20087
ER  - 
TY  - JOUR
A1  - Huege, Jan
A1  - Goetze, Jan
A1  - Schwarz, Doreen
A1  - Bauwe, Hermann
A1  - Hagemann, Martin
A1  - Kopka, Joachim
T1  - Modulation of the major Paths of Carbon in photorespiratory mutants of synechocystis
JF  - PLoS one
N2  - Background: Recent studies using transcript and metabolite profiles of wild-type and gene deletion mutants revealed that photorespiratory pathways are essential for the growth of Synechocystis sp. PCC 6803 under atmospheric conditions. Pool size changes of primary metabolites, such as glycine and glycolate, indicated a link to photorespiration.
 Methodology/Principal Findings: The (13)C labelling kinetics of primary metabolites were analysed in photoautotrophically grown cultures of Synechocystis sp. PCC 6803 by gas chromatography-mass spectrometry (GC-MS) to demonstrate the link with photorespiration. Cells pre-acclimated to high CO(2) (5%, HC) or limited CO(2) (0.035%, LC) conditions were pulse-labelled under very high (2% w/w) (13)C-NaHCO(3) (VHC) conditions followed by treatment with ambient (12)C at HC and LC conditions, respectively. The (13)C enrichment, relative changes in pool size, and (13)C flux of selected metabolites were evaluated. We demonstrate two major paths of CO(2) assimilation via Rubisco in Synechocystis, i.e., from 3PGA via PEP to aspartate, malate and citrate or, to a lesser extent, from 3PGA via glucose-6-phosphate to sucrose. The results reveal evidence of carbon channelling from 3PGA to the PEP pool. Furthermore, (13)C labelling of glycolate was observed under conditions thought to suppress photorespiration. Using the glycolate-accumulating Delta glcD1 mutant, we demonstrate enhanced (13)C partitioning into the glycolate pool under conditions favouring photorespiration and enhanced (13)C partitioning into the glycine pool of the glycine-accumulating Delta gcvT mutant. Under LC conditions, the photorespiratory mutants Delta glcD1 and Delta gcvT showed enhanced activity of the additional carbon-fixing PEP carboxylase pathway.
 Conclusions/Significance: With our approach of non-steady-state (13)C labelling and analysis of metabolite pool sizes with respective (13)C enrichments, we identify the use and modulation of major pathways of carbon assimilation in Synechocystis in the presence of high and low inorganic carbon supplies.
Y1  - 2011
U6  - https://doi.org/10.1371/journal.pone.0016278
SN  - 1932-6203
VL  - 6
IS  - 1
PB  - PLoS
CY  - San Fransisco
ER  -