TY  - JOUR
A1  - Dejonghe, Wim
A1  - Kuenen, Sabine
A1  - Mylle, Evelien
A1  - Vasileva, Mina
A1  - Keech, Olivier
A1  - Viotti, Corrado
A1  - Swerts, Jef
A1  - Fendrych, Matyas
A1  - Ortiz-Morea, Fausto Andres
A1  - Mishev, Kiril
A1  - Delang, Simon
A1  - Scholl, Stefan
A1  - Zarza, Xavier
A1  - Heilmann, Mareike
A1  - Kourelis, Jiorgos
A1  - Kasprowicz, Jaroslaw
A1  - Nguyen, Le Son Long
A1  - Drozdzecki, Andrzej
A1  - Van Houtte, Isabelle
A1  - Szatmari, Anna-Maria
A1  - Majda, Mateusz
A1  - Baisa, Gary
A1  - Bednarek, Sebastian York
A1  - Robert, Stephanie
A1  - Audenaert, Dominique
A1  - Testerink, Christa
A1  - Munnik, Teun
A1  - Van Damme, Daniel
A1  - Heilmann, Ingo
A1  - Schumacher, Karin
A1  - Winne, Johan
A1  - Friml, Jiri
A1  - Verstreken, Patrik
A1  - Russinova, Eugenia
T1  - Mitochondrial uncouplers inhibit clathrin-mediated endocytosis largely through cytoplasmic acidification
JF  - Nature Communications
N2  - ATP production requires the establishment of an electrochemical proton gradient across the inner mitochondrial membrane. Mitochondrial uncouplers dissipate this proton gradient and disrupt numerous cellular processes, including vesicular trafficking, mainly through energy depletion. Here we show that Endosidin9 (ES9), a novel mitochondrial uncoupler, is a potent inhibitor of clathrin-mediated endocytosis (CME) in different systems and that ES9 induces inhibition of CME not because of its effect on cellular ATP, but rather due to its protonophore activity that leads to cytoplasm acidification. We show that the known tyrosine kinase inhibitor tyrphostinA23, which is routinely used to block CME, displays similar properties, thus questioning its use as a specific inhibitor of cargo recognition by the AP-2 adaptor complex via tyrosine motif-based endocytosis signals. Furthermore, we show that cytoplasm acidification dramatically affects the dynamics and recruitment of clathrin and associated adaptors, and leads to reduction of phosphatidylinositol 4,5-biphosphate from the plasma membrane.
Y1  - 2016
U6  - https://doi.org/10.1038/ncomms11710
SN  - 2041-1723
VL  - 7
SP  - 1959
EP  - 1968
PB  - Nature Publ. Group
CY  - London
ER  - 
TY  - GEN
A1  - Cox, Tom
A1  - Maris, Tom
A1  - Soetart, Karline
A1  - Conley, Daniel
A1  - van Damme, Stefan
A1  - Meire, Patrick
A1  - Middelburg, Jack J.
A1  - Vos, Matthijs
A1  - Struyf, Eric
T1  - A macro-tidal freshwater ecosystem recovering from hypereutrophication : the Schelde lease study
N2  - We report a 40 year record of eutrophication and hypoxia on an estuarine ecosystem and its recovery from hypereutrophication. After decades of high inorganic nutrient concentrations and recurring anoxia and hypoxia, we observe a paradoxical increase in chlorophyll-a concentrations with decreasing nutrient inputs. We hypothesise that algal growth was inhibited due to hypereutrophication, either by elevated ammonium concentrations, severe hypoxia or the production of harmful substances in such a reduced environment. We study the dynamics of a simple but realistic mathematical model, incorporating the assumption of algal growth inhibition. It shows a high algal biomass, net oxygen production equilibrium with low ammonia inputs, and a low algal biomass, net oxygen consumption equilibrium with high ammonia inputs. At intermediate ammonia inputs it displays two alternative stable states. Although not intentional, the numerical output of this model corresponds to observations, giving extra support for assumption of algal growth inhibition. Due to potential algal growth inhibition, the recovery of hypereutrophied systems towards a classical eutrophied state, will need reduction of waste loads below certain thresholds and will be accompanied by large fluctuations in oxygen concentrations. We conclude that also flow-through systems, heavily influenced by external forcings which partly mask internal system dynamics, can display multiple stable states.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - paper 144 
KW  - Estuary SW Netherlands
KW  - southern North-Sea
KW  - Past 50 years
KW  - Westerschelde estuary
KW  - Marine ecosystems
Y1  - 2009
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-45180
ER  -