TY  - JOUR
A1  - Putra, Sulistyo Emantoko Dwi
A1  - Tsuprykov, Oleg
A1  - Von Websky, Karoline
A1  - Ritter, Teresa
A1  - Reichetzeder, Christoph
A1  - Hocher, Berthold
T1  - Dealing with large sample sizes: comparison of a new one spot dot blot method to western blot
JF  - Clinical laboratory : the peer reviewed journal for clinical laboratories and laboratories related to blood transfusion
N2  - Background: Western blot is the gold standard method to determine individual protein expression levels. However, western blot is technically difficult to perform in large sample sizes because it is a time consuming and labor intensive process. Dot blot is often used instead when dealing with large sample sizes, but the main disadvantage of the existing dot blot techniques, is the absence of signal normalization to a housekeeping protein.
 Methods: In this study we established a one dot two development signals (ODTDS) dot blot method employing two different signal development systems. The first signal from the protein of interest was detected by horseradish peroxidase (HRP). The second signal, detecting the housekeeping protein, was obtained by using alkaline phosphatase (AP).
 Results: Inter-assay results variations within ODTDS dot blot and western blot and intra-assay variations between both methods were low (1.04 - 5.71%) as assessed by coefficient of variation.
 Conclusions: ODTDS dot blot technique can be used instead of western blot when dealing with large sample sizes without a reduction in results accuracy.
KW  - one dot two development signals (ODTDS) dot blot
KW  - western blot
KW  - protein quantification
KW  - large sample size studies
KW  - comparison
Y1  - 2014
U6  - https://doi.org/10.7754/Clin.Lab.2014.140317
SN  - 1433-6510
VL  - 60
IS  - 11
SP  - 1871
EP  - 1877
PB  - Clin Lab Publ., Verl. Klinisches Labor
CY  - Heidelberg
ER  -