TY - JOUR A1 - Sallen, Jeffrey A1 - Andrä, Christian A1 - Ludyga, Sebastian A1 - Mücke, Manuel A1 - Herrmann, Christian T1 - School children's physical activity, motor competence, and corresponding self-perception BT - a longitudinal analysis of reciprocal relationships JF - Journal of physical activity and health N2 - Background: The relationship between engagement in physical activity and the development of motor competence (MC) is considered to be reciprocal and dynamic throughout childhood and adolescence. The 10-month follow-up study aimed to explore this reciprocal relationship and investigated whether the relationship is mediated by the corresponding self-perception of MC (PMC). Methods: A total of 51 children aged between 10 and 11 years (M = 10.27 [0.45]) participated in the study (52.9% boys, 47.1% girls). As an indicator for physical activity, the average vigorous physical activity (VPA) per day was measured by ActiGraph accelerometers. Two aspects of MC and PMC were recorded: self-movement and object movement. Saturated pathway models in a cross-lagged panel design with 2 measurement points were analyzed. Results: Reciprocal and direct relationships between VPA and MC object movement respectively MC self-movement were not found in longitudinal analyses with PMC as a mediator. Indirect effects of MC at t1 on VPA at t2 via PMC were identified (self-movement: beta = 0.13, 95% confidence interval, 0.04 to 0.26; object movement: beta = 0.14, 95% confidence interval, 0.01 to 0.49). Conclusion: The results highlight the importance of MC and PMC in promoting children's VPA. However, VPA does not drive the development of MC. KW - MOBAK KW - SEMOK KW - fundamental movement skills KW - motor proficiency Y1 - 2020 U6 - https://doi.org/10.1123/jpah.2019-0507 SN - 1543-3080 SN - 1543-5474 VL - 17 IS - 11 SP - 1083 EP - 1090 PB - Human Kinetics Publ. CY - Champaign ER - TY - JOUR A1 - Bösche, Nina Kristine A1 - Rogass, Christian A1 - Lubitz, Christin A1 - Brell, Maximilian A1 - Herrmann, Sabrina A1 - Mielke, Christian A1 - Tonn, Sabine A1 - Appelt, Oona A1 - Altenberger, Uwe A1 - Kaufmann, Hermann T1 - Hyperspectral REE (Rare Earth Element) Mapping of Outcrops-Applications for Neodymium Detection JF - Remote sensing N2 - In this study, an in situ application for identifying neodymium (Nd) enriched surface materials that uses multitemporal hyperspectral images is presented (HySpex sensor). Because of the narrow shape and shallow absorption depth of the neodymium absorption feature, a method was developed for enhancing and extracting the necessary information for neodymium from image spectra, even under illumination conditions that are not optimal. For this purpose, the two following approaches were developed: (1) reducing noise and analyzing changing illumination conditions by averaging multitemporal image scenes and (2) enhancing the depth of the desired absorption band by deconvolving every image spectrum with a Gaussian curve while the rest of the spectrum remains unchanged (Richardson-Lucy deconvolution). To evaluate these findings, nine field samples from the Fen complex in Norway were analyzed using handheld X-ray fluorescence devices and by conducting detailed laboratory-based geochemical rare earth element determinations. The result is a qualitative outcrop map that highlights zones that are enriched in neodymium. To reduce the influences of non-optimal illumination, particularly at the studied site, a minimum of seven single acquisitions is required. Sharpening the neodymium absorption band allows for robust mapping, even at the outer zones of enrichment. From the geochemical investigations, we found that iron oxides decrease the applicability of the method. However, iron-related absorption bands can be used as secondary indicators for sulfidic ore zones that are mainly enriched with rare earth elements. In summary, we found that hyperspectral spectroscopy is a noninvasive, fast and cost-saving method for determining neodymium at outcrop surfaces. Y1 - 2015 U6 - https://doi.org/10.3390/rs70505160 SN - 2072-4292 VL - 7 IS - 5 SP - 5160 EP - 5186 PB - MDPI CY - Basel ER - TY - GEN A1 - Bösche, Nina Kristine A1 - Rogass, Christian A1 - Lubitz, Christin A1 - Brell, Maximilian A1 - Herrmann, Sabrina A1 - Mielke, Christian A1 - Tonn, Sabine A1 - Appelt, Oona A1 - Altenberger, Uwe A1 - Kaufmann, Hermann T1 - Hyperspectral REE (Rare Earth Element) mapping of outcrops BT - applications for neodymium detection N2 - In this study, an in situ application for identifying neodymium (Nd) enriched surface materials that uses multitemporal hyperspectral images is presented (HySpex sensor). Because of the narrow shape and shallow absorption depth of the neodymium absorption feature, a method was developed for enhancing and extracting the necessary information for neodymium from image spectra, even under illumination conditions that are not optimal. For this purpose, the two following approaches were developed: (1) reducing noise and analyzing changing illumination conditions by averaging multitemporal image scenes and (2) enhancing the depth of the desired absorption band by deconvolving every image spectrum with a Gaussian curve while the rest of the spectrum remains unchanged (Richardson-Lucy deconvolution). To evaluate these findings, nine field samples from the Fen complex in Norway were analyzed using handheld X-ray fluorescence devices and by conducting detailed laboratory-based geochemical rare earth element determinations. The result is a qualitative outcrop map that highlights zones that are enriched in neodymium. To reduce the influences of non-optimal illumination, particularly at the studied site, a minimum of seven single acquisitions is required. Sharpening the neodymium absorption band allows for robust mapping, even at the outer zones of enrichment. From the geochemical investigations, we found that iron oxides decrease the applicability of the method. However, iron-related absorption bands can be used as secondary indicators for sulfidic ore zones that are mainly enriched with rare earth elements. In summary, we found that hyperspectral spectroscopy is a noninvasive, fast and cost-saving method for determining neodymium at outcrop surfaces T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 350 KW - rare earth elements KW - imaging spectroscopy KW - neodymium KW - hyperspectral KW - HySpex KW - remote sensing KW - Fen complex Y1 - 2017 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-400171 ER - TY - JOUR A1 - Manzoni, Stefano A1 - Capek, Petr A1 - Porada, Philipp A1 - Thurner, Martin A1 - Winterdahl, Mattias A1 - Beer, Christian A1 - Bruchert, Volker A1 - Frouz, Jan A1 - Herrmann, Anke M. A1 - Lindahl, Bjorn D. A1 - Lyon, Steve W. A1 - Šantrůčková, Hana A1 - Vico, Giulia A1 - Way, Danielle T1 - Reviews and syntheses BT - Carbon use efficiency from organisms to ecosystems - definitions, theories, and empirical evidence JF - Biogeosciences N2 - The cycling of carbon (C) between the Earth surface and the atmosphere is controlled by biological and abiotic processes that regulate C storage in biogeochemical compartments and release to the atmosphere. This partitioning is quantified using various forms of C-use efficiency (CUE) - the ratio of C remaining in a system to C entering that system. Biological CUE is the fraction of C taken up allocated to biosynthesis. In soils and sediments, C storage depends also on abiotic processes, so the term C-storage efficiency (CSE) can be used. Here we first review and reconcile CUE and CSE definitions proposed for autotrophic and heterotrophic organisms and communities, food webs, whole ecosystems and watersheds, and soils and sediments using a common mathematical framework. Second, we identify general CUE patterns; for example, the actual CUE increases with improving growth conditions, and apparent CUE decreases with increasing turnover. We then synthesize > 5000CUE estimates showing that CUE decreases with increasing biological and ecological organization - from uni-cellular to multicellular organisms and from individuals to ecosystems. We conclude that CUE is an emergent property of coupled biological-abiotic systems, and it should be regarded as a flexible and scale-dependent index of the capacity of a given system to effectively retain C. Y1 - 2018 U6 - https://doi.org/10.5194/bg-15-5929-2018 SN - 1726-4170 SN - 1726-4189 VL - 15 IS - 19 SP - 5929 EP - 5949 PB - Copernicus CY - Göttingen ER - TY - JOUR A1 - Quiterio, Ana A1 - Martins, Joao A1 - Onofre, Marcos A1 - Costa, Joao A1 - Rodrigues, Joao Mota A1 - Gerlach, Erin A1 - Scheur, Claude A1 - Herrmann, Christian T1 - MOBAK 1 assessment in primary physical education BT - exploring basic motor competences of portuguese 6-Year-Olds JF - Perceptual & motor skills N2 - Children’s motor competence is known to have a determinant role in learning and engaging later in complex motor skills and, thus, in physical activity. The development of adequate motor competence is a central aim of physical education, and assuring that pupils are learning and developing motor competence depends on accurate assessment protocols. The MOBAK 1 test battery is a recent instrument developed to assess motor competence in primary physical education. This study used the MOBAK 1 to explore motor competence levels and gender differences among 249 (Mage = 6.3, SD = 0.5 years; 127 girls and 122 boys) Grade 1 primary school Portuguese children. On independent sample t tests, boys presented higher object movement motor competence than girls (boys: M = 5.8, SD = 1.7; girls: M = 4.0, SD = 1.7; p < .001), while girls were more proficient among self-movement skills (girls: M = 5.1, SD = 1.8; boys: M = 4.3, SD = 1.7; p < .01). On “total motor competence,” boys (M = 10.3, SD = 2.6) averaged one point ahead of girls (M = 9.1, SD = 2.9). The percentage of girls in the first quartile of object movement was 18.9%, while, for “self movement,” the percentage of boys in the first quartile was almost double that of girls (30.3% and 17.3%, respectively). The confirmatory model to test for construct validity confirmed the assumed theoretical two-factor structure of MOBAK 1 test items in this Portuguese sample. These results support the MOBAK 1 instrument for assessing motor competence and highlighted gender differences, of relevance to intervention efforts. KW - physical education KW - MOBAK 1 KW - instrument KW - assessment KW - motor competence Y1 - 2018 U6 - https://doi.org/10.1177/0031512518804358 SN - 0031-5125 SN - 1558-688X VL - 125 IS - 6 SP - 1055 EP - 1069 PB - Sage Publ. CY - Thousand Oaks ER - TY - GEN A1 - Bierbach, David A1 - Schulte, Matthias A1 - Herrmann, Nina A1 - Tobler, Michael A1 - Stadler, Stefan A1 - Jung, Christian T. A1 - Kunkel, Benjamin A1 - Riesch, Rüdiger A1 - Klaus, Sebastian A1 - Ziege, Madlen A1 - Indy, Jeane Rimber A1 - Arias-Rodriguez, Lenin A1 - Plath, Martin T1 - Predator-induced changes of female mating preferences BT - innate and experiential effects T2 - Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe N2 - Background In many species males face a higher predation risk than females because males display elaborate traits that evolved under sexual selection, which may attract not only females but also predators. Females are, therefore, predicted to avoid such conspicuous males under predation risk. The present study was designed to investigate predator-induced changes of female mating preferences in Atlantic mollies (Poecilia mexicana). Males of this species show a pronounced polymorphism in body size and coloration, and females prefer large, colorful males in the absence of predators. Results In dichotomous choice tests predator-naïve (lab-reared) females altered their initial preference for larger males in the presence of the cichlid Cichlasoma salvini, a natural predator of P. mexicana, and preferred small males instead. This effect was considerably weaker when females were confronted visually with the non-piscivorous cichlid Vieja bifasciata or the introduced non-piscivorous Nile tilapia (Oreochromis niloticus). In contrast, predator experienced (wild-caught) females did not respond to the same extent to the presence of a predator, most likely due to a learned ability to evaluate their predators' motivation to prey. Conclusions Our study highlights that (a) predatory fish can have a profound influence on the expression of mating preferences of their prey (thus potentially affecting the strength of sexual selection), and females may alter their mate choice behavior strategically to reduce their own exposure to predators. (b) Prey species can evolve visual predator recognition mechanisms and alter their mate choice only when a natural predator is present. (c) Finally, experiential effects can play an important role, and prey species may learn to evaluate the motivational state of their predators. T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 984 KW - sexual selection KW - female choice KW - non-independent mate choice KW - predator recognition KW - Poecilia mexicana Y1 - 2020 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-431099 SN - 1866-8372 IS - 984 ER - TY - THES A1 - Herrmann, Christian T1 - Motorische Basiskompetenzen - Konstrukt, Erfassung und Forschungsstand Y1 - 2017 ER - TY - JOUR A1 - Ristow, Michael A1 - Herrmann, Andreas A1 - Illig, Hubert A1 - Klemm, Gunther A1 - Kummer, Volker A1 - Kläge, Hans-Christian A1 - Machatzi, Bernd A1 - Raetzel, Stefan A1 - Schwarz, R. A1 - Zimmermann, Friedrich T1 - Liste und Rote Liste der etablierten Gefäßpflanzen Brandenburgs Y1 - 2006 ER - TY - JOUR A1 - Sallen, Jeffrey A1 - Hirschmann, Florian A1 - Herrmann, Christian T1 - Evaluation and Adaption of the Trier Inventory for Chronic Stress (TICS) for Assessment in Competitive Sports JF - Frontiers in psychology N2 - The demands of a career in competitive sports can lead to chronic stress perception among athletes if there is a non-conformity of requirements and available coping resources. The Trier Inventory for Chronic Stress (TICS) (Schulz et al., 2004) is said to be thoroughly validated. Nevertheless, it has not yet been subjected to a confirmatory factor analysis. The present study aims (1) to evaluate the factorial validity of the TICS within the context of competitive sports and (2) to adapt a short version (TICS-36). The total sample consisted of 564 athletes (age in years: M = 19.1, SD = 3.70). The factor structure of the original TICS did not adequately fit the present data, whereas the short version presented a satisfactory fit. The results indicate that the TICS-36 is an economical instrument for gathering interpretable information about chronic stress. For assessment in competitive sports with TICS-36, we generated overall and gender-specific norm values. KW - chronic stressors KW - mental health KW - athletes KW - stress measurement KW - Olympic sports KW - factor analysis KW - measurement invariance Y1 - 2018 U6 - https://doi.org/10.3389/fpsyg.2018.00308 SN - 1664-1078 VL - 9 PB - Frontiers Research Foundation CY - Lausanne ER - TY - JOUR A1 - Steinberg, Christian E. W. A1 - Ouerghemmi, Nadia A1 - Herrmann, Steffen A1 - Bouchnak, Rihab A1 - Timofeyev, Maxim A. A1 - Menzel, Ralph T1 - Stress by poor food quality and exposure to humic substances : daphnia magna responds with oxidative stress, lifespan extension, but reduced offspring numbers N2 - In freshwater systems, many abiotic and biotic factors determine the natural fluctuation of Daphnia spec. populations: climatic and water quality parameters, quantitative and qualitative food quality and quantity, predation, and humic substances. Many factors/stressors act in concert. In this contribution, we supplied Daphnia magna with two different diets (chlorococcal alga Pseudokirchneriella subcapitata and baker's yeast) fed ad libitum and exposed it to an environmentally realistic concentration of humic substances (HSs). Exposure to HSs caused a transcriptionally controlled stress response with studied genes; cat and hsp60, for the latter partial sequences have been identified. Furthermore, the exposure to HSs reduced the antioxidant capacity. Yet, a much stronger oxidative stress is caused by feeding yeast, which reduced the anti-oxidative capacity to values of approximately 50% of the green algal diet. This reduction is most likely due to the yeast's cell wall to resist digestion rather than to the elemental ratio or deficiency in long-chained unsaturated fatty acids, because both diets were deficient in fatty acids with back bones of more than 20 C-atoms. We assume that the biochemical machinery in the gut continuously activated oxygen to cleave the yeast's cell wall and, hence, reduced the antioxidative capacity of the animals. Neither the analyzed oxidant, H2O2, nor the antioxidants, total apparent ascorbic acid nor free proline, reflected the oxidative stress situations properly. In addition to the stress, HS exposure extended the mean lifespan of algae-fed D. magna, but at the expense of offspring numbers; so did also the pure yeast diet as compared to the algae diet. The first lifespan extension can be explained by the potential of HSs to block the pathway via the insulin-like growth factor 1 (IGF), whereas the second matches the, in aging papers, well described, but mechanistically poorly understood caloric restriction. Yeast-fed animals, exposed to HSs changed the energy allocation by reducing life span, but increasing offspring numbers. With the lifespan and offspring numbers, ecologically relevant parameters are differently affected by the simultaneous action of two environmentally relevant stressors. Y1 - 2010 UR - http://www.springerlink.com/content/100271 U6 - https://doi.org/10.1007/s10750-010-0334-4 SN - 0018-8158 ER - TY - JOUR A1 - Bierbach, David A1 - Schulte, Matthias A1 - Herrmann, Nina A1 - Tobler, Michael A1 - Stadler, Stefan A1 - Jung, Christian T. A1 - Kunkel, Benjamin A1 - Riesch, Rüdiger A1 - Klaus, Sebastian A1 - Ziege, Madlen A1 - Rimber Indy, Jeane A1 - Arias-Rodriguez, Lenin A1 - Plath, Martin T1 - Predator-induced changes of female mating preferences innate and experiential effects JF - BMC evolutionary biology N2 - Background: In many species males face a higher predation risk than females because males display elaborate traits that evolved under sexual selection, which may attract not only females but also predators. Females are, therefore, predicted to avoid such conspicuous males under predation risk. The present study was designed to investigate predator-induced changes of female mating preferences in Atlantic mollies (Poecilia mexicana). Males of this species show a pronounced polymorphism in body size and coloration, and females prefer large, colorful males in the absence of predators. Results: In dichotomous choice tests predator-naive (lab-reared) females altered their initial preference for larger males in the presence of the cichlid Cichlasoma salvini, a natural predator of P. mexicana, and preferred small males instead. This effect was considerably weaker when females were confronted visually with the non-piscivorous cichlid Vieja bifasciata or the introduced non-piscivorous Nile tilapia (Oreochromis niloticus). In contrast, predator experienced (wild-caught) females did not respond to the same extent to the presence of a predator, most likely due to a learned ability to evaluate their predators' motivation to prey. Conclusions: Our study highlights that (a) predatory fish can have a profound influence on the expression of mating preferences of their prey (thus potentially affecting the strength of sexual selection), and females may alter their mate choice behavior strategically to reduce their own exposure to predators. (b) Prey species can evolve visual predator recognition mechanisms and alter their mate choice only when a natural predator is present. (c) Finally, experiential effects can play an important role, and prey species may learn to evaluate the motivational state of their predators. KW - Sexual selection KW - female choice KW - non-independent mate choice KW - predator recognition KW - Poecilia mexicana Y1 - 2011 U6 - https://doi.org/10.1186/1471-2148-11-190 SN - 1471-2148 VL - 11 IS - 3-4 PB - BioMed Central CY - London ER - TY - JOUR A1 - Schröder, Rolf A1 - Fürst, Dieter Oswald A1 - Klasen, Christian A1 - Reiman, Jens A1 - Herrmann, Harald A1 - VanDerVen, Peter F. M. T1 - The association of plectin with Z-discs is a prerequisite for the formation of the intermyofibrillar desmin cytoskeleton Y1 - 2000 ER - TY - JOUR A1 - Schröder, Rolf A1 - Warlo, Irene A1 - Herrmann, Harald A1 - VanDerVen, Peter F. M A1 - Klasen, Christian A1 - Blümke, Ingmar A1 - Mundegar, Rustam R. A1 - Fürst, Dieter Oswald A1 - Göbel, Hans H. A1 - Magin, Thomas T1 - Immunogold EM reveals a close association of plectin and the desmin cytoskeleton in human skeletal muscle Y1 - 1999 ER - TY - GEN A1 - Manzoni, Stefano A1 - Čapek, Petr A1 - Porada, Philipp A1 - Thurner, Martin A1 - Winterdahl, Mattias A1 - Beer, Christian A1 - Brüchert, Volker A1 - Frouz, Jan A1 - Herrmann, Anke M. A1 - Lindahl, Björn D. A1 - Lyon, Steve W. A1 - Šantrůčková, Hana A1 - Vico, Giulia A1 - Way, Danielle T1 - Reviews and syntheses BT - carbon use efficiency from organisms to ecosystems – definitions, theories, and empirical evidence T2 - Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe N2 - The cycling of carbon (C) between the Earth surface and the atmosphere is controlled by biological and abiotic processes that regulate C storage in biogeochemical compartments and release to the atmosphere. This partitioning is quantified using various forms of C-use efficiency (CUE) - the ratio of C remaining in a system to C entering that system. Biological CUE is the fraction of C taken up allocated to biosynthesis. In soils and sediments, C storage depends also on abiotic processes, so the term C-storage efficiency (CSE) can be used. Here we first review and reconcile CUE and CSE definitions proposed for autotrophic and heterotrophic organisms and communities, food webs, whole ecosystems and watersheds, and soils and sediments using a common mathematical framework. Second, we identify general CUE patterns; for example, the actual CUE increases with improving growth conditions, and apparent CUE decreases with increasing turnover. We then synthesize > 5000CUE estimates showing that CUE decreases with increasing biological and ecological organization - from uni-cellular to multicellular organisms and from individuals to ecosystems. We conclude that CUE is an emergent property of coupled biological-abiotic systems, and it should be regarded as a flexible and scale-dependent index of the capacity of a given system to effectively retain C. T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 1134 KW - gross primary production KW - net primary production KW - plant respiration KW - microbial carbon KW - stoichiometric controls KW - growth efficiency KW - bacterial growth KW - excess carbon KW - soil KW - matter Y1 - 2021 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-446386 SN - 1866-8372 IS - 1134 ER - TY - JOUR A1 - Aichert, Ingrid A1 - Staiger, Anja A1 - Schulte-Mäter, Anne A1 - Becker-Redding, Ulrike A1 - Stahn, Corinna A1 - Peschke, Claudia A1 - Heide, Judith A1 - Ott, Susan A1 - Herrmann, Heike A1 - Völsch, Juliane A1 - Mayer, Jörg A1 - Rohnke, Lucie A1 - Frank, Ulrike A1 - Stadie, Nicole A1 - Jentsch, Nadine A1 - Blech, Anke A1 - Kurtenbach, Stephanie A1 - Thieke, Johanna A1 - Schröder, Astrid A1 - Stahn, Corinna A1 - Hörnig, Robin A1 - Burchert, Frank A1 - De Bleser, Ria A1 - Heister, Julian A1 - Bartels, Luise A1 - Würzner, Kay-Michael A1 - Böhme, Romy A1 - Burmester, Juliane A1 - Krajewski, Melanie A1 - Nager, Wido A1 - Jungehülsing, Gerhard Jan A1 - Wartenburger, Isabell A1 - Jöbges, Michael A1 - Schwilling, Eleonore A1 - Lidzba, Karen A1 - Winkler, Susanne A1 - Konietzko, Andreas A1 - Krägeloh-Mann, Ingeborg A1 - Rilling, Eva A1 - Wilken, Rainer A1 - Wismann, Kathrin A1 - Glandorf, Birte A1 - Hoffmann, Hannah A1 - Hinnenkamp, Christiane A1 - Rohlmann, Insa A1 - Ludewigt, Jacqueline A1 - Bittner, Christian A1 - Orlov, Tatjana A1 - Claus, Katrin A1 - Ehemann, Christine A1 - Winnecken, Andreas A1 - Hummel, Katja A1 - Breitenstein, Sarah ED - Wahl, Michael ED - Stahn, Corinna ED - Hanne, Sandra ED - Fritzsche, Tom T1 - Spektrum Patholinguistik = Schwerpunktthema: Von der Programmierung zur Artikulation : Sprechapraxie bei Kindern und Erwachsenen N2 - Das 3. Herbsttreffen Patholinguistik fand am 21. November 2009 an der Universität Potsdam statt. Der vorliegende Tagungsband enthält die drei Hauptvorträge zum Schwerpunktthema „Von der Programmierung zu Artikulation: Sprechapraxie bei Kindern und Erwachsenen“. Darüber hinaus enthält der Band die Beiträge aus dem Spektrum Patholinguistik, sowie die Abstracts der Posterpräsentationen. N2 - The 3rd Herbsttreffen Patholinguistik was held on November 21st, 2009 at the University of Potsdam. These proceedings contain the three main lectures of the central topic „From programming to articulation: Apraxia of speech of children and adults “. Additionally this volume contains the contributions of Spektrum Patholinguistik, as well as the abstracts of the poster presentations. T3 - Spektrum Patholinguistik - 3 KW - Patholinguistik KW - Sprechapraxie KW - Sprachtherapie KW - patholinguistics KW - apraxia of speech KW - speech and language therapy Y1 - 2010 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-45470 SN - 978-3-86956-079-3 SN - 1869-3822 SN - 1866-9433 IS - 3 PB - Universitätsverlag Potsdam CY - Potsdam ER - TY - JOUR A1 - Welke, Robert-William A1 - Sperber, Hannah Sabeth A1 - Bergmann, Ronny A1 - Koikkarah, Amit A1 - Menke, Laura A1 - Sieben, Christian A1 - Krüger, Detlev H. A1 - Chiantia, Salvatore A1 - Herrmann, Andreas A1 - Schwarzer, Roland T1 - Characterization of hantavirus N protein intracellular dynamics and localization JF - Viruses N2 - Hantaviruses are enveloped viruses that possess a tri-segmented, negative-sense RNA genome. The viral S-segment encodes the multifunctional nucleocapsid protein (N), which is involved in genome packaging, intracellular protein transport, immunoregulation, and several other crucial processes during hantavirus infection. In this study, we generated fluorescently tagged N protein constructs derived from Puumalavirus (PUUV), the dominant hantavirus species in Central, Northern, and Eastern Europe. We comprehensively characterized this protein in the rodent cell line CHO-K1, monitoring the dynamics of N protein complex formation and investigating co-localization with host proteins as well as the viral glycoproteins Gc and Gn. We observed formation of large, fibrillar PUUV N protein aggregates, rapidly coalescing from early punctate and spike-like assemblies. Moreover, we found significant spatial correlation of N with vimentin, actin, and P-bodies but not with microtubules. N constructs also co-localized with Gn and Gc albeit not as strongly as the glycoproteins associated with each other. Finally, we assessed oligomerization of N constructs, observing efficient and concentration-dependent multimerization, with complexes comprising more than 10 individual proteins. KW - hantavirus KW - N protein KW - oligomerization KW - actin KW - P-bodies KW - vimentin KW - Number and Brightness KW - Puumalavirus KW - macromolecular assemblies Y1 - 2022 U6 - https://doi.org/10.3390/v14030457 SN - 1999-4915 VL - 14 IS - 3 PB - MDPI CY - Basel ER -