TY  - JOUR
A1  - Beta, Carsten
A1  - Bodenschatz, Eberhard
T1  - Microfluidic tools for quantitative studies of eukaryotic chemotaxis
JF  - European journal of cell biology
N2  - Over the past decade, microfluidic techniques have been established as a versatile platform to perform live cell experiments under well-controlled conditions. To investigate the directional responses of cells, stable concentration profiles of chemotactic factors can be generated in microfluidic gradient mixers that provide a high degree of spatial control. However, the times for built-up and switching of gradient profiles are in general too slow to resolve the intracellular protein translocation events of directional sensing of eukaryotes. Here, we review an example of a conventional microfluidic gradient mixer as well as the novel flow photolysis technique that achieves an increased temporal resolution by combining the photo-activation of caged compounds with the advantages of microfluidic chambers.
KW  - Eukaryotic chemotaxis
KW  - Dictyostelium discoideum
KW  - Microfluidics
KW  - Caged compounds
KW  - Numerical simulations
Y1  - 2011
U6  - https://doi.org/10.1016/j.ejcb.2011.05.006
SN  - 0171-9335
VL  - 90
IS  - 10
SP  - 811
EP  - 816
PB  - Elsevier
CY  - Jena
ER  -