TY - GEN A1 - Guardado-Calvo, Pablo A1 - Bignon, Eduardo A. A1 - Stettner, Eva A1 - Jeffers, Scott Allen A1 - Pérez-Vargas, Jimena A1 - Pehau-Arnaudet, Gerard A1 - Tortorici, M. Alejandra A1 - Jestin, Jean- Luc A1 - England, Patrick A1 - Tischler, Nicole D. A1 - Rey, Félix A. T1 - Mechanistic insight into bunyavirus-induced membrane fusion from structure-function analyses of the hantavirus envelope glycoprotein Gc T2 - Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe N2 - Hantaviruses are zoonotic viruses transmitted to humans by persistently infected rodents, giving rise to serious outbreaks of hemorrhagic fever with renal syndrome (HFRS) or of hantavirus pulmonary syndrome (HPS), depending on the virus, which are associated with high case fatality rates. There is only limited knowledge about the organization of the viral particles and in particular, about the hantavirus membrane fusion glycoprotein Gc, the function of which is essential for virus entry. We describe here the X-ray structures of Gc from Hantaan virus, the type species hantavirus and responsible for HFRS, both in its neutral pH, monomeric pre-fusion conformation, and in its acidic pH, trimeric post-fusion form. The structures confirm the prediction that Gc is a class II fusion protein, containing the characteristic beta-sheet rich domains termed I, II and III as initially identified in the fusion proteins of arboviruses such as alpha-and flaviviruses. The structures also show a number of features of Gc that are distinct from arbovirus class II proteins. In particular, hantavirus Gc inserts residues from three different loops into the target membrane to drive fusion, as confirmed functionally by structure-guided mutagenesis on the HPS-inducing Andes virus, instead of having a single "fusion loop". We further show that the membrane interacting region of Gc becomes structured only at acidic pH via a set of polar and electrostatic interactions. Furthermore, the structure reveals that hantavirus Gc has an additional N-terminal "tail" that is crucial in stabilizing the post-fusion trimer, accompanying the swapping of domain III in the quaternary arrangement of the trimer as compared to the standard class II fusion proteins. The mechanistic understandings derived from these data are likely to provide a unique handle for devising treatments against these human pathogens. T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 676 KW - Semliki-forest-virus KW - Borne encephalitis-virus KW - N-linked glycosylation KW - human dendritic cells KW - Valley fever virus KW - Hantaan-virus KW - Hemorrhagic-fever KW - crystal-structure KW - electron crytomography KW - pulmonary syndrome Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-411599 SN - 1866-8372 IS - 676 ER - TY - JOUR A1 - Guardado-Calvo, Pablo A1 - Bignon, Eduardo A. A1 - Stettner, Eva A1 - Jeffers, Scott Allen A1 - Perez-Vargas, Jimena A1 - Pehau-Arnaudet, Gerard A1 - Tortoric, M. Alejandra A1 - Jestin, Jean-Luc A1 - England, Patrick A1 - Tischler, Nicole D. A1 - Rey, Felix A. T1 - Mechanistic Insight into Bunyavirus-Induced Membrane Fusion from Structure-Function Analyses of the Hantavirus Envelope Glycoprotein Gc JF - PLoS Pathogens N2 - Hantaviruses are zoonotic viruses transmitted to humans by persistently infected rodents, giving rise to serious outbreaks of hemorrhagic fever with renal syndrome (HFRS) or of hantavirus pulmonary syndrome (HPS), depending on the virus, which are associated with high case fatality rates. There is only limited knowledge about the organization of the viral particles and in particular, about the hantavirus membrane fusion glycoprotein Gc, the function of which is essential for virus entry. We describe here the X-ray structures of Gc from Hantaan virus, the type species hantavirus and responsible for HFRS, both in its neutral pH, monomeric pre-fusion conformation, and in its acidic pH, trimeric post-fusion form. The structures confirm the prediction that Gc is a class II fusion protein, containing the characteristic beta-sheet rich domains termed I, II and III as initially identified in the fusion proteins of arboviruses such as alpha-and flaviviruses. The structures also show a number of features of Gc that are distinct from arbovirus class II proteins. In particular, hantavirus Gc inserts residues from three different loops into the target membrane to drive fusion, as confirmed functionally by structure-guided mutagenesis on the HPS-inducing Andes virus, instead of having a single "fusion loop". We further show that the membrane interacting region of Gc becomes structured only at acidic pH via a set of polar and electrostatic interactions. Furthermore, the structure reveals that hantavirus Gc has an additional N-terminal "tail" that is crucial in stabilizing the post-fusion trimer, accompanying the swapping of domain III in the quaternary arrangement of the trimer as compared to the standard class II fusion proteins. The mechanistic understandings derived from these data are likely to provide a unique handle for devising treatments against these human pathogens. Y1 - 2016 U6 - https://doi.org/10.1371/journal.ppat.1005813 SN - 1553-7366 SN - 1553-7374 VL - 12 SP - 153 EP - 166 PB - PLoS CY - San Fransisco ER - TY - BOOK A1 - Calvo, D. A1 - Schulze, Bert-Wolfgang T1 - Operators on Corner Manifolds with Exit to Infinity T3 - Preprint / Universität Potsdam, Institut für Mathematik, Arbeitsgruppe Partiell Y1 - 2005 SN - 1437-739X PB - Univ. CY - Potsdam ER - TY - INPR A1 - Calvo, D. A1 - Schulze, Bert-Wolfgang T1 - Operators on corner manifolds with exit to infinity N2 - We study (pseudo-)differential operators on a manifold with edge Z, locally modelled on a wedge with model cone that has itself a base manifold W with smooth edge Y . The typical operators A are corner degenerate in a specific way. They are described (modulo ‘lower order terms’) by a principal symbolic hierarchy σ(A) = (σ ψ(A), σ ^(A), σ ^(A)), where σ ψ is the interior symbol and σ ^(A)(y, η), (y, η) 2 T*Y \ 0, the (operator-valued) edge symbol of ‘first generation’, cf. [15]. The novelty here is the edge symbol σ^ of ‘second generation’, parametrised by (z, Ϛ) 2 T*Z \ 0, acting on weighted Sobolev spaces on the infinite cone with base W. Since such a cone has edges with exit to infinity, the calculus has the problem to understand the behaviour of operators on a manifold of that kind. We show the continuity of corner-degenerate operators in weighted edge Sobolev spaces, and we investigate the ellipticity of edge symbols of second generation. Starting from parameter-dependent elliptic families of edge operators of first generation, we obtain the Fredholm property of higher edge symbols on the corresponding singular infinite model cone. T3 - Preprint - (2005) 01 KW - Operators on manifolds with edge and conical exit to infinity KW - Sobolev spaces with double weights on singular cones Y1 - 2005 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-29753 ER - TY - INPR A1 - Calvo, D. A1 - Schulze, Bert-Wolfgang T1 - Edge symbolic structures of second generation N2 - Operators on a manifold with (geometric) singularities are degenerate in a natural way. They have a principal symbolic structure with contributions from the different strata of the configuration. We study the calculus of such operators on the level of edge symbols of second generation, based on specific quantizations of the corner-degenerate interior symbols, and show that this structure is preserved under compositions. T3 - Preprint - (2005) 18 KW - Operators on manifolds with second order singularities KW - edge quantizations KW - continuity in Sobolev spaces with double weights Y1 - 2005 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-29940 ER - TY - BOOK A1 - Calvo, D. A1 - Martin, Calin-Iulian A1 - Schulze, Bert-Wolfgang T1 - Symbolic Structures on Corner Manifolds T3 - Preprint / Universität Potsdam, Institut für Mathematik, Arbeitsgruppe Partiell Y1 - 2004 SN - 1437-739X PB - Univ. CY - Potsdam ER -