TY  - JOUR
A1  - Schatz, J.
A1  - Ohlendorf, B.
A1  - Busse, P.
A1  - Pelz, G.
A1  - Dolch, D.
A1  - Teubner, J.
A1  - Encarnacao, Jorge A.
A1  - Muehle, Ralf -Udo
A1  - Fischer, M.
A1  - Hoffmann, B.
A1  - Kwasnitschka, L.
A1  - Balkema-Buschmann, Anne
A1  - Mettenleiter, Thomas Christoph
A1  - Mueller, T.
A1  - Freuling, C. M.
T1  - Twenty years of active bat rabies surveillance in Germany
BT  - a detailed analysis and future perspectives
JF  - Epidemiology and infection
N2  - In Germany, active bat rabies surveillance was conducted between 1993 and 2012. A total of 4546 oropharyngeal swab samples from 18 bat species were screened for the presence of EBLV-1- , EBLV-2- and BBLV-specific RNA. Overall, 0 center dot 15% of oropharyngeal swab samples tested EBLV-1 positive, with the majority originating from Eptesicus serotinus. Interestingly, out of seven RT-PCR-positive oropharyngeal swabs subjected to virus isolation, viable virus was isolated from a single serotine bat (E. serotinus). Additionally, about 1226 blood samples were tested serologically, and varying virus neutralizing antibody titres were found in at least eight different bat species. The detection of viral RNA and seroconversion in repeatedly sampled serotine bats indicates long-term circulation of the virus in a particular bat colony. The limitations of random-based active bat rabies surveillance over passive bat rabies surveillance and its possible application of targeted approaches for future research activities on bat lyssavirus dynamics and maintenance are discussed.
KW  - surveillance
KW  - epidemiology
KW  - Bat rabies
KW  - lyssavirus
Y1  - 2014
U6  - https://doi.org/10.1017/S0950268813002185
SN  - 0950-2688
SN  - 1469-4409
VL  - 142
IS  - 6
SP  - 1155
EP  - 1166
PB  - Cambridge Univ. Press
CY  - New York
ER  - 
TY  - JOUR
A1  - Gläser, Stefanie P.
A1  - Bolte, Kathrin
A1  - Martin, Karin
A1  - Busse, Hans-Jürgen
A1  - Grossart, Hans-Peter
A1  - Kämpfer, Peter
A1  - Gläser, Jens
T1  - Novosphingobium fuchskuhlense sp nov., isolated from the north-east basin of Lake Grosse Fuchskuhle
JF  - International journal of systematic and evolutionary microbiology
N2  - A yellow pigmented, Gram-negative, rod-shaped bacterium designated FNE08-7(T) was isolated from subsurface water of the north-east basin of the bog lake Grosse Fuchskuhle (Brandenburg, Germany). A first analysis of the nearly full-length 16S rRNA gene sequence analysis including environmental 16S rRNA gene sequences derived from freshwater ecosystems showed that strain FNE08-7(T) is the first cultured representative, to our knowledge, of the freshwater tribe Novo-A2. Further analysis indicates highest 16S rRNA gene sequence similarities to the type strains of Novosphingobium stygium (98.0%) and Novosphingobium taihuense (97.4%) and between 94.0% and 96.9% sequence similarity to other members of the genus Novosphingobium. Reconstruction of phylogenetic trees showed that strain FNE08-7(T) formed a distinct cluster with the type strains of N. stygium and N. taihuense supported by high bootstrap values. DNA DNA hybridization of strain FNE08-7(T) with N. stygium SMCC B0712(T) and N. taihuense DSM 17507(T) revealed low similarity values of 18.4% (reciprocal: 11.4%) and 23.1% (reciprocal: 54.2%), respectively. The predominant fatty acid of the isolate is C-18:1 omega 7c (56.4%) and two characteristic 2-hydroxy fatty acids, C-14:0 2-OH (16.5%) and C-15:0 2-OH (3.3%) occur. Ubiquinone Q-10 is the major respiratory quinone. The predominant polar lipids are phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylglycerol, sphingoglycolipid, phosphatidylcholine and minor amounts of diphosphatidylglycerol. Spermidine is the predominant polyamine. Characterization by genotypic, chemotaxonomic and phenotypic analysis indicate that strain FNE08-7(T) represents a novel species of the genus Novosphingobium within the Alphaproteobacteria. Therefore, we propose the species Novosphingobium fuchskuhlense sp. nov., with FNE08-7(T) (=DSM 25065(T)=CCM 7978(T)=CCUG 61508(T)) as the type strain.
Y1  - 2013
U6  - https://doi.org/10.1099/ijs.0.043083-0
SN  - 1466-5026
VL  - 63
SP  - 586
EP  - 592
PB  - Society for General Microbiology
CY  - Reading
ER  -