TY  - JOUR
A1  - Bleidorn, Christoph
A1  - Lanterbecq, Deborah
A1  - Eeckhaut, Igor
A1  - Tiedemann, Ralph
T1  - A PCR survey of Hox genes in the myzostomid Myzostoma cirriferum
N2  - Using degenerate primers, we were able to identify seven Hox genes for the myzostomid Myzostoma cirriferum. The recovered fragments belong to anterior class (Mci_lab, Mci_pb), central class (Mci_Dfd, Mci_Lox5, Mci_Antp, Mci_Lox4), and posterior class (Mci_Post2) paralog groups. Orthology assignment was verified by phylogenetic analyses and presence of diagnostic regions in the homeodomain as well as flanking regions. The presence of Lox5, Lox4, and Post2 supports the inclusion of Myzostomida within Lophotrochozoa. We found signature residues within flanking regions of Lox5, which are also found in annelids, but not in Platyhelminthes. As such the available Hox genes data of myzostomids support an annelid relationship.
Y1  - 2009
UR  - http://www.springerlink.com/content/100526
U6  - https://doi.org/10.1007/s00427-009-0282-z
SN  - 0949-944X
ER  - 
TY  - GEN
A1  - Bleidorn, Christoph
A1  - Podsiadlowski, Lars
A1  - Zhong, Min
A1  - Eeckhaut, Igor
A1  - Hartmann, Stefanie
A1  - Halanych, Kenneth M.
A1  - Tiedemann, Ralph
T1  - On the phylogenetic position of Myzostomida : can 77 genes get it wrong?
N2  - Background: Phylogenomic analyses recently became popular to address questions about deep metazoan phylogeny. Ribosomal proteins (RP) dominate many of these analyses or are, in some cases, the only genes included. Despite initial hopes, hylogenomic analyses including tens to hundreds of genes still fail to robustly place many bilaterian taxa. Results: Using the phylogenetic position of myzostomids as an example, we show that phylogenies derived from RP genes and mitochondrial genes produce incongruent results. Whereas the former support a position within a clade of platyzoan taxa, mitochondrial data recovers an annelid affinity, which is strongly supported by the gene order data and is congruent with morphology. Using hypothesis testing, our RP data significantly rejects the annelids affinity, whereas a platyzoan relationship is significantly rejected by the mitochondrial data. Conclusion: We conclude (i) that reliance of a set of markers belonging to a single class of macromolecular complexes might bias the analysis, and (ii) that concatenation of all available data might introduce conflicting signal into phylogenetic analyses. We therefore strongly recommend testing for data incongruence in phylogenomic analyses. Furthermore, judging all available data, we consider the annelid affinity hypothesis more plausible than a possible platyzoan affinity for myzostomids, and suspect long branch attraction is influencing the RP data. However, this hypothesis needs further confirmation by future analyses.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - paper 123 
KW  - Cirriferum myzostomida
KW  - Mitochondrial genomes
KW  - Transfer-rna
KW  - Data sets
KW  - Sequence
Y1  - 2009
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-44893
ER  -