TY  - JOUR
A1  - Piluso, Susanna
A1  - Hiebl, Bernhard
A1  - Gorb, Stanislav N.
A1  - Kovalev, Alexander
A1  - Lendlein, Andreas
A1  - Neffe, Axel T.
T1  - Hyaluronic acid-based hydrogels crosslinked by copper-catalyzed azide-alkyne cycloaddition with tailorable mechanical properties
JF  - The international journal of artificial organs
N2  - Biopolymers of the extracellular matrix are attractive starting materials for providing degradable and biocompatible biomaterials. In this study, hyaluronic acid-based hydrogels with tunable mechanical properties were prepared by the use of copper-catalyzed azide-alkyne cycloaddition (known as "click chemistry"). Alkyne-functionalized hyaluronic acid was crosslinked with linkers having two terminal azide functionalities, varying crosslinker density as well as the lengths and rigidity of the linker molecules. By variation of the crosslinker density and crosslinker type, hydrogels with elastic moduli in the range of 0.5-4 kPa were prepared. The washed materials contained a maximum of 6.8 mg copper per kg dry weight and the eluate of the gel crosslinked with diazidostilbene did not show toxic effects on L929 cells. The hyaluronic acid-based hydrogels have potential as biomaterials for cell culture or soft tissue regeneration applications.
KW  - Biomaterial
KW  - Hydrogel
KW  - Hyaluronic acid
KW  - Microindentation
KW  - Rheology
Y1  - 2011
U6  - https://doi.org/10.5301/IJAO.2011.6394
SN  - 0391-3988
VL  - 34
IS  - 2
SP  - 192
EP  - 197
PB  - Wichtig
CY  - Milano
ER  - 
TY  - JOUR
A1  - Shkilnyy, Andriy
A1  - Gräf, Ralph
A1  - Hiebl, Bernhard
A1  - Neffe, Axel T.
A1  - Friedrich, Alwin
A1  - Hartmann, Juergen
A1  - Taubert, Andreas
T1  - Unprecedented, low cytotoxicity of spongelike calcium phosphate/poly(ethylene imine) hydrogel composites
N2  - Covalently crosslinked PEI hydrogels are efficient templates for calcium phosphate mineralization in SBF. In contrast to the PEI hydrogels, non-crosslinked PEI does not lead to calcium phosphate nucleation and growth in SBF. The precipitate is a mixture of brushite and hydroxyapatite. The PEI/calcium phosphate composite material exhibits a sponge like morphology and a chemical composition that is interesting for implants. Cytotoxicity tests using Dictyostelium discoideum amoebae show that both the non-mineralized and mineralized hydrogels have a very low cytotoxicity. This suggests that next generation PEI hydrogels, where also the degradation products are non-toxic, could be interesting for biomedical applications.
Y1  - 2009
UR  - http://www3.interscience.wiley.com/cgi-bin/jhome/77002860
U6  - https://doi.org/10.1002/mabi.200800266
SN  - 1616-5187
ER  -