TY  - JOUR
A1  - Auyyuenyong, Ratchada
A1  - Henze, Andrea
A1  - Ungru, Julia
A1  - Schweigert, Florian Johannes
A1  - Raila, Jens
A1  - Vervuert, Ingrid
T1  - Determination of lipid profiles in serum of obese ponies before and after weight reduction by using multi-one-dimensional thin-layer chromatography
JF  - Research in veterinary science
N2  - Obesity is a key component of equine metabolic syndrome, which is highly associated with laminitis. Feed restriction and/or exercise are known to alleviate the detrimental effects of insulin resistance in obese ponies. However, little is known about changes in the serum lipid patterns due to weight reduction and its association with disease outcomes. Therefore, the lipid patterns in the serum of 14 mature ponies before and after a 14-week body weight reduction program (BWRP) were investigated by multi-one-dimensional thin-layer chromatography (MOD-TLC). Additionally, sensitivity to insulin (SI), body condition scores (BCS) and cresty neck scores (CNS) were measured. A BWRP resulted in a significant loss of body weight (P < 0.001), which was associated with beneficial decreases in BCS and CNS (both, P < 0.001). Serum lipid compositions revealed significantly increased free fatty acid (FFA), sphingomyelin (SM; both P < 0.001), total cholesterol (C) and cholesterol ester (CE) (both P < 0.01) and triacylglycerol (TG; P < 0.05) densities. Improvement of SI after the BWRP was associated with increases in neutral lipids (C, CE and TG, all P < 0.01), FFA and the phospholipid SM (both, P < 0.001). The results show that a BWRP in obese ponies was effective and associated with changes in the concentrations of neutral lipids and the phospholipid SM, indicating that SM may play a role in insulin signaling pathways and thus in the pathogenesis of insulin resistance and the progression of metabolic syndrome in obese ponies.
KW  - Neutral lipids
KW  - Equine metabolic syndrome
KW  - Phospholipids
KW  - Horse
KW  - Thin layer chromatography
Y1  - 2017
U6  - https://doi.org/10.1016/j.rvsc.2017.11.013
SN  - 0034-5288
SN  - 1532-2661
VL  - 117
SP  - 111
EP  - 117
PB  - Elsevier
CY  - Oxford
ER  - 
TY  - JOUR
A1  - Kwanbunjan, Karunee
A1  - Panprathip, Pornpimol
A1  - Phosat, Chanchira
A1  - Chumpathat, Noppanath
A1  - Wechjakwen, Naruemon
A1  - Puduang, Somchai
A1  - Auyyuenyong, Ratchada
A1  - Henkel, Ina
A1  - Schweigert, Florian J.
T1  - Association of retinol binding protein 4 and transthyretin with triglyceride levels and insulin resistance in rural thais with high type 2 diabetes risk
JF  - BMC Endocrine Disorders
N2  - Background: Retinol binding protein 4 (RBP4), a protein secreted by adipocytes and bound in plasma to transthyretin (TTR), has been associated with obesity, the early phase of insulin resistance, metabolic syndrome, and type 2 diabetes mellitus. The objective of this study was to elucidate the relationship between RBP4, TTR, triglyceride (TG) and type 2 diabetes risk in rural Thailand. Results: RBP4 and TTR levels, as well as homeostatic model assessment of insulin resistance (HOMA-IR) values, were significantly elevated among subjects with high triglyceride levels (p < 0.01, p < 0.05, p < 0.05, respectively). Triglyceride levels correlated with RBP4 (r = 0.34, p < 0.001) and TTR (r= 0.26, p < 0.01) levels, as well as HOMA-IR values (r= 0.16, p < 0.05). After adjustment for age and gender, the risk of hypertriglyceridemia was 3.7 times greater (95% Cl =1.42 -9.73, p = 0.008) in the highest RBP4 tertile as compared to the lowest tertile. Similarly, the highest TTR and HOMA-IR tertiles had greater risk of hypertriglyceridemia at 3.5 (95% Cl = 1.30-9.20, p = 0.01) and 3.6 (95% CI = 1.33- 9.58, p = 0.01) times higher than the respective lowest tertiles. The correlation between TTR and blood glucose was statistically significant (r 0.18, p < 0.05), but not found this relationship in RBP4. Conclusions: The associations of RBP4 and TTR with hypertriglyceridemia and insulin resistance may have important implications for the risk of heart disease and stroke.
KW  - RBP4
KW  - TTR
KW  - HOMA-IR
KW  - Hypertriglyceridemia
KW  - Type 2 diabetes
Y1  - 2018
U6  - https://doi.org/10.1186/s12902-018-0254-2
SN  - 1472-6823
VL  - 18
PB  - BMC
CY  - London
ER  -