TY - JOUR A1 - Muschalla, Beate A1 - Henning, Anne A1 - Haake, Tim Woody A1 - Cornetz, Kathrin A1 - Olbrich, Dieter T1 - Mental health problem or workplace problem or something else BT - what contributes to work perception? JF - Disability and rehabilitation : an international, multidisciplinary journal N2 - Purpose: Work perception is an important predictor for work ability and, therefore, of interest for rehabilitation. Until now it is unclear to which extent different psychological aspects explain work perception. This study investigates in which way workplace problems on the one hand, and mental health and coping on the other hand, contribute to work perception. Methods: A heterogeneous sample of 384 persons in working age with and without mental health problems was recruited. Participants gave self-reports on workplace problems, mental health problems, work-coping, work-anxiety, and work perception. Results: Persons with mental health problems and workplace problems (M + W) perceive the highest degree of work demands, followed by persons with workplace problems but without mental health problems (NM + W). Work-anxiety appeared as the strongest factor explaining perception of high work demands, whereas general mental health problems did not contribute significantly to variance explanation. Conclusions: Persons with specific mental health problems in terms of work-anxiety may be expected to perceive higher work demands. They may be detected when asking for work perception, e.g., within the frame of return-to-work interventions in rehabilitation, or in occupational health settings by mental hazard analysis. KW - work ability KW - work anxiety KW - workplace KW - mental disorders KW - rehabilitation KW - work perception Y1 - 2018 U6 - https://doi.org/10.1080/09638288.2018.1501099 SN - 0963-8288 SN - 1464-5165 VL - 42 IS - 4 SP - 502 EP - 509 PB - Routledge, Taylor & Francis Group CY - Abingdon ER - TY - JOUR A1 - Markschies, Christoph A1 - Päßler, Ulrich A1 - Grote, Mathias A1 - Greenwood MacKinney, Anne A1 - Kusber, Wolf-Henning A1 - Jahn, Regine A1 - Damaschun, Ferdinand A1 - Böhme, Katrin ED - Ette, Ottmar ED - Knobloch, Eberhard ED - Päßler, Ulrich T1 - HiN : Alexander von Humboldt im Netz = Christian Gottfried Ehrenberg BT - Lebensbilder eines Naturforschers N2 - -Christoph Markschies: Geleitwort -Ulrich Päßler: Christian Gottfried Ehrenberg: Lebensbilder eines Naturforschers -Mathias Grote: „Aus dem Kleinen bauen sich die Welten“ – Christian Gottfried Ehrenbergs ökologische Mikrobiologie avant la lettre -Anne Greenwood MacKinney: Die Inszenierung naturforschender Gelehrsamkeit beim Sammeln: Christian Gottfried Ehrenbergs und Wilhelm Hemprichs nordafrikanische Forschungsreise (1820 – 1825) -Ulrich Päßler: Reisen im Nahen Osten. Zeichnungen -Ulrich Päßler: Christian Gottfried Ehrenberg und die Biogeographie: Die russisch-sibirische Reise mit Alexander von Humboldt (1829) -Ulrich Päßler: Russisch-Sibirische Reise. Zeichnungen -Wolf-Henning Kusber, Regine Jahn: Christian Gottfried Ehrenbergs Zeichnungen: Eine frühe wissenschaftliche Dokumentation mikroskopischer Organismen -Ferdinand Damaschun: Christian Gottfried Ehrenberg und die Entwicklung der Mikroskop-Technik im 19. Jahrhundert -Ulrich Päßler: Die Reise ins Kleinste der Natur. Zeichnungen -Katrin Böhme: Das große Ganze: Christian Gottfried Ehrenberg und die Gesellschaft Naturforschender Freunde zu Berlin T3 - HiN : Alexander von Humboldt im Netz ; International Review for Humboldtian Studies - XXII. 2021, 42 KW - Christian Gottfried Ehrenberg KW - Naturgemälde KW - Mikrobiologie KW - Infusionsthierchen KW - Alexander von Humboldt KW - Wissenschaftsgeschichte KW - Forschungsreisen KW - Mikroskopie KW - Ehrenberg KW - Ökologie KW - Cholera KW - Protistologie KW - Infektion KW - Infusorium KW - Wilhelm Hemprich KW - gelehrte Tugenden KW - Sammlungspraxis KW - Dokumentationspraxis KW - wissenschaftliche Zeichnungen KW - Biogeographie KW - Algen KW - Artbeschreibung KW - Biodiversität KW - naturkundliche Sammlung KW - Glas KW - Infusorien KW - Mikrogeologie KW - Chevalier KW - Pistor & Schiek KW - Geschichte der Mikroskopie KW - wissenschaftliche Instrumente KW - Gesellschaft Naturforschender Freunde zu Berlin KW - GNF KW - Infusorienwerke Y1 - 2021 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-501413 SN - 2568-3543 SN - 1617-5239 VL - XXII IS - 42 PB - Universitätsverlag Potsdam CY - Potsdam ER - TY - JOUR A1 - Dondapati, Srujan Kumar A1 - Lübberding, Henning A1 - Zemella, Anne A1 - Thoring, Lena A1 - Wüstenhagen, Doreen Anja A1 - Kubick, Stefan T1 - Functional Reconstitution of Membrane Proteins Derived From Eukaryotic Cell-Free Systems JF - Frontiers in pharmacology N2 - Cell-free protein synthesis (CFPS) based on eukaryotic Sf21 lysate is gaining interest among researchers due to its ability to handle the synthesis of complex human membrane proteins (MPs). Additionally Sf21 cell-free systems contain endogenous microsomal vesicles originally derived from the endoplasmic reticulum (ER). After CFPS, MPs will be translocated into the microsomal vesicles membranes present in the lysates. Thus microsomal membranes offer a natural environment for de novo synthesized MPs. Despite the advantage of synthesizing complex MPs with post translational modifications directly into the microsomal membranes without any additional solubilization supplements, batch based Sf21 cell-free synthesis suffers from low yields. The bottleneck for MPs in particular after the synthesis and incorporation into the microsomal membranes is to analyze their functionality. Apart from low yields of the synthesized MPs with batch based cell-free synthesis, the challenges arise in the form of cytoskeleton elements and peripheral endogenous proteins surrounding the microsomes which may impede the functional analysis of the synthesized proteins. So careful sample processing after the synthesis is particularly important for developing the appropriate functional assays. Here we demonstrate how MPs (native and batch synthesized) from ER derived microsomes can be processed for functional analysis by electrophysiology and radioactive uptake assay methods. Treatment of the microsomal membranes either with a sucrose washing step in the case of human serotonin transporter (hSERT) and sarco/endoplasmic reticulum Ca2+/ATPase (SERCA) pump or with mild detergents followed by the preparation of proteoliposomes in the case of the human voltage dependent anionic channel (hVDAC1) helps to analyze the functional properties of MPs. KW - membrane proteins KW - Sf21 lysates KW - microsomes KW - cell-free protein synthesis KW - proteoliposomes KW - transporter KW - ion channel KW - pump Y1 - 2019 U6 - https://doi.org/10.3389/fphar.2019.00917 SN - 1663-9812 VL - 10 PB - Frontiers Research Foundation CY - Lausanne ER -