TY  - JOUR
A1  - Heilmann, Katja
A1  - Groth, Thomas
A1  - Behrsing, Olaf
A1  - Wagner, Albrecht
A1  - Schossig-Tiedemann, Michael
A1  - Lendlein, Andreas
A1  - Micheel, Burkhard
T1  - The influence of the chemical composition of cell culture material on the growth and antibody production of hybridoma cells
N2  - The multiplication and antibody production of murine hybridoma cells cultured on five different polymer membranes were tested and compared with conventional tissue culture polystyrene (TCPS). Membranes were prepared from polyacrylonitrile (PAN) and acrylonitrile copolymerized with N-vinylpyrrolidone (NVP20, NVP30), Na-methallylsulfonate (NaMAS) and N-(3-amino-propyl-methacrylamide-hydrochloride) (APMA). Cell number and antibody concentration were quantified as criteria for viability and productivity. Adhesion of hybridoma cells was characterized by vital and scanning electron microscopy. The results suggest that a strong adhesion of cells, observed on APMA and TCPS, increased cell growth but reduced monoclonal antibody production. In contrast membranes with lowered adhesivity such as NVP20 provided favourable conditions for monoclonal antibody production. In addition it was shown that this membrane also possessed a minor fouling as indicated by the low decrease of water flux across the membrane after protein adsorption. It was concluded that NVP20 could be a suitable material for the development of hollow fibre membranes for bioreactors.
Y1  - 2005
UR  - http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T3C-4DPYNGY- 4&_coverDate=02%2F09%2F2005&_alid=268995355&_rdoc=1&_fmt=&_orig=search&_qd=1&_cdi=4943&_sort=d&view=c&_acct=C000053886&_v e
ER  - 
TY  - JOUR
A1  - Rickert, D
A1  - Lendlein, Andreas
A1  - Kelch, S
A1  - Franke, R. P.
A1  - Moses, M. A.
T1  - Cell proliferation and cellular activity of primary cell cultures of the oral cavity after cell seeding on the surface of a degradable, thermoplastic block copolymer
N2  - Using standard cell biological and biochemical methods we were able to test the ability of a degradable, thermoplastic block copolymer to support the adhesion, proliferation, and the cellular activity of primary cell cultures of the oral cavity in vitro. The delicate balance between a group of endogenous enzymes, Matrix Metalloproteinases (MMPs), and their inhibitors (Tissue Inhibitor of MMPs, TIMPs) have a decisive function in the remodeling of the extracellular matrix during processes like wound healing or the integration of biomaterials in surrounding tissues after implantation. Recently developed, biodegradable thermoplastic elastomers with shape-memory properties may be the key to develop new therapeutical options in head and neck surgery. Primary cell cultures of the oral cavity of Sprague-Dawley rats were seeded on the surface of a thermoplastic block copolymer and on a polystyrene surface as control. Conditioned media of the primary cells were analyzed for MMPs and TIMPs after different periods of cell growth. The MMP and TIMP expression was analysed by zymography and a radiometric enzyme assay. No statistically significant differences in the appearance and the kinetic of MMP-1, MMP-2, MMP-9 and TIMPs were detected between cells grown on the polymer surface compared to the control. An appropriate understanding of the molecular processes that regulate cellular growth and integration of a biomaterial in surrounding tissue is the requirement for an optimal adaptation of biodegradable, polymeric biomaterials to the physiological, anatomical, and surgical conditions in vivo to develop new therapeutic options in otolaryngology and head and neck surgery
Y1  - 2005
ER  - 
TY  - JOUR
A1  - Feng, Y.
A1  - Kelch, S.
A1  - Rickert, D.
A1  - Fuhrmann, R.
A1  - Franke, R. P.
A1  - Lendlein, Andreas
T1  - Biokompatible abbaubare Formgedächtnispolymersysteme als intelligente Implantatmaterialien
Y1  - 2004
ER  - 
TY  - JOUR
A1  - Kelch, S.
A1  - Lendlein, Andreas
A1  - Schulte, J.
T1  - Kunststoffe mit Formgedächtnis : die erstaunlichen Fähigkeiten intelligenter Materialien
Y1  - 2004
SN  - 0344-5690
ER  - 
TY  - JOUR
A1  - Lendlein, Andreas
A1  - Kelch, S.
A1  - Schulte, J.
A1  - Kratz, K.
T1  - Shape-memory polymers
Y1  - 2004
ER  - 
TY  - JOUR
A1  - Groth, Thomas
A1  - Lendlein, Andreas
T1  - In-vivo-Reparatur von Blutgefäßen durch alternierende Adsorption von Polyelektrolyten
Y1  - 2004
UR  - http://www3.interscience.wiley.com/cgi-bin/fulltext/107614315/PDFSTART
ER  - 
TY  - JOUR
A1  - Santoso, F.
A1  - Schroeter, M.
A1  - Wagner, Albrecht
A1  - Lendlein, Andreas
A1  - Sckomaecker, R.
T1  - Simultane Funktionalisierung und Porenöffnung von Polyetherimid-Membranen zur Entwicklung neuer Trägermaterialien für die Apharese
Y1  - 2004
ER  - 
TY  - JOUR
A1  - Groth, Thomas
A1  - Lendlein, Andreas
T1  - Layer-by-layer deposition of polyelectrolytes : a versatile tool for the in vivo repair of blood vessels and the preparation of biocompatible implant coatings
Y1  - 2004
ER  - 
TY  - JOUR
A1  - Rickert, D.
A1  - Lendlein, Andreas
A1  - Kelch, S.
A1  - Moses, M. A.
A1  - Franke, R. P.
T1  - Biokompatibilitätstestung von bioabbaubaren Shape Memory Polymeren in vivo
Y1  - 2004
ER  - 
TY  - JOUR
A1  - Binzen, Eva
A1  - Lendlein, Andreas
A1  - Kelch, S.
A1  - Rickert, D.
A1  - Franke, R. P.
T1  - Biomaterial-microvasculature interaction on polymers after implantation in mice
Y1  - 2004
ER  -