TY - JOUR A1 - Kienzler, Andrea Altevogt Nee A1 - Flehr, Roman A1 - Gehne, Sören A1 - Kumke, Michael Uwe A1 - Bannwarth, Willi T1 - Verification and biophysical characterization of a New Three-Color Forster Resonance-Energy-Transfer (FRET) System in DNA JF - Helvetica chimica acta N2 - We report on a new three-color FRET system consisting of three fluorescent dyes, i.e., of a carbostyril (=quinolin-2(1H)-one)-derived donor D, a (bathophenanthroline)ruthenium complex as a relay chromophore A1, and a Cy dye as A2 (FRET=Forster resonance-energy-transfer) (cf. Fig. 1). With their widely matching spectroscopic properties (cf. Fig. 2), the combination of these dyes yielded excellent FRET efficiencies. Furthermore, fluorescence lifetime measurements revealed that the long fluorescence lifetime of the Ru complex was transferred to the Cy dye offering the possibility to measure the whole system in a time-resolved mode. The FRET system was established on double-stranded DNA (cf. Fig. 3) but it should also be generally applicable to other biomolecules. KW - Forster resonance energy transfer (FRET) system KW - DNA KW - Fluorescence KW - Ruthenium complexes Y1 - 2012 U6 - https://doi.org/10.1002/hlca.201100460 SN - 0018-019X VL - 95 IS - 4 SP - 543 EP - 555 PB - Wiley-VCH CY - Weinheim ER - TY - JOUR A1 - Kienzler, Andrea A1 - Flehr, Roman A1 - Kramer, Rolf A. A1 - Gehne, Soeren A1 - Kumke, Michael Uwe A1 - Bannwarth, Willi T1 - Novel Three-Color FRET Tool Box for Advanced Protein and DNA Analysis JF - Bioconjugate chemistry N2 - We report on a new three-color FRET system which we were able to verify in peptides as well as in synthetic DNA. All three chromophores could be introduced by a building block approach avoiding postsynthetic labeling. Additional features are robustness, matching spectroscopic properties, high-energy transfer, and sensitivity. The system was investigated in detail on a set of peptides as well as an array of tailored oligonucleotides. The detailed analysis of the experimental data and comparison with theoretical considerations were in excellent agreement. It is shown that in the case of polypeptides specific interaction with the fluorescence probes has to be considered. In contrast with DNA, the fluorescence probes did not show any indications of such interactions. The novel three-color FRET toolbox revealed the potential for applications studying fundamental processes of three interacting molecules in life science applications. Y1 - 2011 U6 - https://doi.org/10.1021/bc2002659 SN - 1043-1802 VL - 22 IS - 9 SP - 1852 EP - 1863 PB - American Chemical Society CY - Washington ER - TY - JOUR A1 - Gehne, Sören A1 - Flehr, Roman A1 - Kienzler, Andrea Altevogt Nee A1 - Berg, Maik A1 - Bannwarth, Willi A1 - Kumke, Michael Uwe T1 - Dye dynamics in three-color FRET samples JF - The journal of physical chemistry : B, Condensed matter, materials, surfaces, interfaces & biophysical chemistry N2 - Time-resolved emission data (fluorescence decay and fluorescence depolarization) of two three-color Forster resonance energy transfer (tc-FRET) systems consisting of a carbostyril donor (D), a ruthenium complex (Ru) as relay dye, and a Cy5 derivative (Cy) or, optionally, an anthraquinone quencher (Q) were carefully analyzed using advanced distribution analysis models. Thereby, it is possible to get information on the flexibility and mobility of the chromophores which are bound to double stranded (ds) DNA. Especially the distance distribution based on the analysis of the fluorescence depolarization is an attractive approach to complement data of fluorescence decay time analysis. The distance distributions extracted from the experimental data were in excellent agreement with those determined from accessible volume (AV) simulations. Moreover, the study showed that for tc-FRET systems the combination of dyes emitting on different time scales (e.g., nanoseconds vs microseconds) is highly beneficial in the distribution analysis of time-resolved luminescence data in cases where macromolecules such as DNA are involved. Here, the short lifetimes can yield information on the rotation of the dye molecule itself and the long lifetime can give insight in the overall dynamics of the macromolecule. Y1 - 2012 U6 - https://doi.org/10.1021/jp3064273 SN - 1520-6106 VL - 116 IS - 35 SP - 10798 EP - 10806 PB - American Chemical Society CY - Washington ER - TY - JOUR A1 - Philips, Andrea A1 - Walz, Ariane A1 - Bergner, Andreas G. N. A1 - Gräff, Thomas A1 - Heistermann, Maik A1 - Kienzler, Sarah A1 - Korup, Oliver A1 - Lipp, Torsten A1 - Schwanghart, Wolfgang A1 - Zeilinger, Gerold T1 - Immersive 3D geovisualization in higher education JF - Journal of geography in higher education N2 - In this study, we investigate how immersive 3D geovisualization can be used in higher education. Based on MacEachren and Kraak's geovisualization cube, we examine the usage of immersive 3D geovisualization and its usefulness in a research-based learning module on flood risk, called GEOSimulator. Results of a survey among participating students reveal benefits, such as better orientation in the study area, higher interactivity with the data, improved discourse among students and enhanced motivation through immersive 3D geovisualization. This suggests that immersive 3D visualization can effectively be used in higher education and that 3D CAVE settings enhance interactive learning between students. KW - immersive 3D geovisualization KW - 3D CAVE KW - higher education KW - learning success KW - student survey KW - flood risk Y1 - 2015 U6 - https://doi.org/10.1080/03098265.2015.1066314 SN - 0309-8265 SN - 1466-1845 VL - 39 IS - 3 SP - 437 EP - 449 PB - Routledge, Taylor & Francis Group CY - Abingdon ER -