TY  - JOUR
A1  - Botero, David
A1  - Monk, Jonathan
A1  - Rodriguez Cubillos, Maria Juliana
A1  - Rodriguez Cubillos, Andres Eduardo
A1  - Restrepo, Mariana
A1  - Bernal-Galeano, Vivian
A1  - Reyes, Alejandro
A1  - Gonzalez Barrios, Andres
A1  - Palsson, Bernhard O.
A1  - Restrepo, Silvia
A1  - Bernal, Adriana
T1  - Genome-scale metabolic model of Xanthomonas phaseoli pv. manihotis
BT  - an approach to elucidate pathogenicity at the metabolic level
JF  - Frontiers in genetics
N2  - Xanthomonas phaseoli pv. manihotis (Xpm) is the causal agent of cassava bacterial blight, the most important bacterial disease in this crop. There is a paucity of knowledge about the metabolism of Xanthomonas and its relevance in the pathogenic process, with the exception of the elucidation of the xanthan biosynthesis route. Here we report the reconstruction of the genome-scale model of Xpm metabolism and the insights it provides into plant-pathogen interactions. The model, iXpm1556, displayed 1,556 reactions, 1,527 compounds, and 890 genes. Metabolic maps of central amino acid and carbohydrate metabolism, as well as xanthan biosynthesis of Xpm, were reconstructed using Escher (https://escher.github.io/) to guide the curation process and for further analyses. The model was constrained using the RNA-seq data of a mutant of Xpm for quorum sensing (QS), and these data were used to construct context-specific models (CSMs) of the metabolism of the two strains (wild type and QS mutant). The CSMs and flux balance analysis were used to get insights into pathogenicity, xanthan biosynthesis, and QS mechanisms. Between the CSMs, 653 reactions were shared; unique reactions belong to purine, pyrimidine, and amino acid metabolism. Alternative objective functions were used to demonstrate a trade-off between xanthan biosynthesis and growth and the re-allocation of resources in the process of biosynthesis. Important features altered by QS included carbohydrate metabolism, NAD(P)(+) balance, and fatty acid elongation. In this work, we modeled the xanthan biosynthesis and the QS process and their impact on the metabolism of the bacterium. This model will be useful for researchers studying host-pathogen interactions and will provide insights into the mechanisms of infection used by this and other Xanthomonas species.
KW  - Xanthomonas
KW  - Xpm
KW  - cassava bacterial blight
KW  - genome-scale metabolic
KW  - model
KW  - quorum sensing
Y1  - 2020
U6  - https://doi.org/10.3389/fgene.2020.00837
SN  - 1664-8021
VL  - 11
PB  - Frontiers Media
CY  - Lausanne
ER  - 
TY  - CHAP
A1  - Jacqmin, Julien
A1  - Özdemir, Paker Doğu
A1  - Fell Kurban, Caroline
A1  - Tunç Pekkan, Zelha
A1  - Koskinen, Johanna
A1  - Suonpää, Maija
A1  - Seng, Cheyvuth
A1  - Carlon, May Kristine Jonson
A1  - Gayed, John Maurice
A1  - Cross, Jeffrey S.
A1  - Langseth, Inger
A1  - Jacobsen, Dan Yngve
A1  - Haugsbakken, Halvdan
A1  - Bethge, Joseph
A1  - Serth, Sebastian
A1  - Staubitz, Thomas
A1  - Wuttke, Tobias
A1  - Nordemann, Oliver
A1  - Das, Partha-Pratim
A1  - Meinel, Christoph
A1  - Ponce, Eva
A1  - Srinath, Sindhu
A1  - Allegue, Laura
A1  - Perach, Shai
A1  - Alexandron, Giora
A1  - Corti, Paola
A1  - Baudo, Valeria
A1  - Turró, Carlos
A1  - Moura Santos, Ana
A1  - Nilsson, Charlotta
A1  - Maldonado-Mahauad, Jorge
A1  - Valdiviezo, Javier
A1  - Carvallo, Juan Pablo
A1  - Samaniego-Erazo, Nicolay
A1  - Poce, Antonella
A1  - Re, Maria Rosaria
A1  - Valente, Mara
A1  - Karp Gershon, Sa’ar
A1  - Ruipérez-Valiente, José A.
A1  - Despujol, Ignacio
A1  - Busquets, Jaime
A1  - Kerr, John
A1  - Lorenz, Anja
A1  - Schön, Sandra
A1  - Ebner, Martin
A1  - Wittke, Andreas
A1  - Beirne, Elaine
A1  - Nic Giolla Mhichíl, Mairéad
A1  - Brown, Mark
A1  - Mac Lochlainn, Conchúr
A1  - Topali, Paraskevi
A1  - Chounta, Irene-Angelica
A1  - Ortega-Arranz, Alejandro
A1  - Villagrá-Sobrino, Sara L.
A1  - Martínez-Monés, Alejandra
A1  - Blackwell, Virginia Katherine
A1  - Wiltrout, Mary Ellen
A1  - Rami Gaddem, Mohamed
A1  - Hernández Reyes, César Augusto
A1  - Nagahama, Toru
A1  - Buchem, Ilona
A1  - Okatan, Ebru
A1  - Khalil, Mohammad
A1  - Casiraghi, Daniela
A1  - Sancassani, Susanna
A1  - Brambilla, Federica
A1  - Mihaescu, Vlad
A1  - Andone, Diana
A1  - Vasiu, Radu
A1  - Şahin, Muhittin
A1  - Egloffstein, Marc
A1  - Bothe, Max
A1  - Rohloff, Tobias
A1  - Schenk, Nathanael
A1  - Schwerer, Florian
A1  - Ifenthaler, Dirk
A1  - Hense, Julia
A1  - Bernd, Mike
ED  - Meinel, Christoph
ED  - Staubitz, Thomas
ED  - Schweiger, Stefanie
ED  - Friedl, Christian
ED  - Kiers, Janine
ED  - Ebner, Martin
ED  - Lorenz, Anja
ED  - Ubachs, George
ED  - Mongenet, Catherine
ED  - Ruipérez-Valiente, José A.
ED  - Cortes Mendez, Manoel
T1  - EMOOCs 2021
N2  - From June 22 to June 24, 2021, Hasso Plattner Institute, Potsdam, hosted the seventh European MOOC Stakeholder Summit (EMOOCs 2021) together with the eighth ACM Learning@Scale Conference.
Due to the COVID-19 situation, the conference was held fully online.
The boost in digital education worldwide as a result of the pandemic was also one of the main topics of this year’s EMOOCs. All institutions of learning have been forced to transform and redesign their educational methods, moving from traditional models to hybrid or completely online models at scale. The learnings, derived from practical experience and research, have been explored in EMOOCs 2021 in six tracks and additional workshops, covering various aspects of this field. In this publication, we present papers from the conference’s Experience Track, the Policy Track, the Business Track, the International Track, and the Workshops.
KW  - e-learning
KW  - microcredential
KW  - MOOC
KW  - digital education
KW  - experience
KW  - online course design
KW  - online course creation
KW  - higher education
Y1  - 2021
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-510300
SN  - 978-3-86956-512-5
PB  - Universitätsverlag Potsdam
CY  - Potsdam
ER  -