TY  - JOUR
A1  - Scheller, Frieder W.
A1  - Scheller, A.
T1  - Bi-Enzymelektrode zur Messung von Sorbitol in pharmazeutischen Produkten
Y1  - 1996
ER  - 
TY  - JOUR
A1  - Banks, Jo Ann
A1  - Nishiyama, Tomoaki
A1  - Hasebe, Mitsuyasu
A1  - Bowman, John L.
A1  - Gribskov, Michael
A1  - dePamphilis, Claude
A1  - Albert, Victor A.
A1  - Aono, Naoki
A1  - Aoyama, Tsuyoshi
A1  - Ambrose, Barbara A.
A1  - Ashton, Neil W.
A1  - Axtell, Michael J.
A1  - Barker, Elizabeth
A1  - Barker, Michael S.
A1  - Bennetzen, Jeffrey L.
A1  - Bonawitz, Nicholas D.
A1  - Chapple, Clint
A1  - Cheng, Chaoyang
A1  - Correa, Luiz Gustavo Guedes
A1  - Dacre, Michael
A1  - DeBarry, Jeremy
A1  - Dreyer, Ingo
A1  - Elias, Marek
A1  - Engstrom, Eric M.
A1  - Estelle, Mark
A1  - Feng, Liang
A1  - Finet, Cedric
A1  - Floyd, Sandra K.
A1  - Frommer, Wolf B.
A1  - Fujita, Tomomichi
A1  - Gramzow, Lydia
A1  - Gutensohn, Michael
A1  - Harholt, Jesper
A1  - Hattori, Mitsuru
A1  - Heyl, Alexander
A1  - Hirai, Tadayoshi
A1  - Hiwatashi, Yuji
A1  - Ishikawa, Masaki
A1  - Iwata, Mineko
A1  - Karol, Kenneth G.
A1  - Koehler, Barbara
A1  - Kolukisaoglu, Uener
A1  - Kubo, Minoru
A1  - Kurata, Tetsuya
A1  - Lalonde, Sylvie
A1  - Li, Kejie
A1  - Li, Ying
A1  - Litt, Amy
A1  - Lyons, Eric
A1  - Manning, Gerard
A1  - Maruyama, Takeshi
A1  - Michael, Todd P.
A1  - Mikami, Koji
A1  - Miyazaki, Saori
A1  - Morinaga, Shin-ichi
A1  - Murata, Takashi
A1  - Müller-Röber, Bernd
A1  - Nelson, David R.
A1  - Obara, Mari
A1  - Oguri, Yasuko
A1  - Olmstead, Richard G.
A1  - Onodera, Naoko
A1  - Petersen, Bent Larsen
A1  - Pils, Birgit
A1  - Prigge, Michael
A1  - Rensing, Stefan A.
A1  - Mauricio Riano-Pachon, Diego
A1  - Roberts, Alison W.
A1  - Sato, Yoshikatsu
A1  - Scheller, Henrik Vibe
A1  - Schulz, Burkhard
A1  - Schulz, Christian
A1  - Shakirov, Eugene V.
A1  - Shibagaki, Nakako
A1  - Shinohara, Naoki
A1  - Shippen, Dorothy E.
A1  - Sorensen, Iben
A1  - Sotooka, Ryo
A1  - Sugimoto, Nagisa
A1  - Sugita, Mamoru
A1  - Sumikawa, Naomi
A1  - Tanurdzic, Milos
A1  - Theissen, Guenter
A1  - Ulvskov, Peter
A1  - Wakazuki, Sachiko
A1  - Weng, Jing-Ke
A1  - Willats, William W. G. T.
A1  - Wipf, Daniel
A1  - Wolf, Paul G.
A1  - Yang, Lixing
A1  - Zimmer, Andreas D.
A1  - Zhu, Qihui
A1  - Mitros, Therese
A1  - Hellsten, Uffe
A1  - Loque, Dominique
A1  - Otillar, Robert
A1  - Salamov, Asaf
A1  - Schmutz, Jeremy
A1  - Shapiro, Harris
A1  - Lindquist, Erika
A1  - Lucas, Susan
A1  - Rokhsar, Daniel
A1  - Grigoriev, Igor V.
T1  - The selaginella genome identifies genetic changes associated with the evolution of vascular plants
JF  - Science
N2  - Vascular plants appeared similar to 410 million years ago, then diverged into several lineages of which only two survive: the euphyllophytes (ferns and seed plants) and the lycophytes. We report here the genome sequence of the lycophyte Selaginella moellendorffii (Selaginella), the first nonseed vascular plant genome reported. By comparing gene content in evolutionarily diverse taxa, we found that the transition from a gametophyte- to a sporophyte-dominated life cycle required far fewer new genes than the transition from a nonseed vascular to a flowering plant, whereas secondary metabolic genes expanded extensively and in parallel in the lycophyte and angiosperm lineages. Selaginella differs in posttranscriptional gene regulation, including small RNA regulation of repetitive elements, an absence of the trans-acting small interfering RNA pathway, and extensive RNA editing of organellar genes.
Y1  - 2011
U6  - https://doi.org/10.1126/science.1203810
SN  - 0036-8075
VL  - 332
IS  - 6032
SP  - 960
EP  - 963
PB  - American Assoc. for the Advancement of Science
CY  - Washington
ER  - 
TY  - JOUR
A1  - Scheller, Frieder W.
A1  - Makower, Alexander
A1  - Bier, Frank Fabian
A1  - Wollenberger, Ursula
A1  - Ghindilis, A. L.
A1  - Eremenko, A. V.
A1  - Pfeiffer, Dorothea
T1  - Enzymsensoren zur Bestimmung subnanomolarer Konzentrationen
Y1  - 1995
ER  - 
TY  - JOUR
A1  - Freaney, R.
A1  - MacShane, A.
A1  - Keaveny, T. V.
A1  - MacKenna, M.
A1  - Rabenstein, K.
A1  - Scheller, Frieder W.
A1  - Pfeiffer, Dorothea
A1  - Urban, G.
A1  - Moser, I.
A1  - Jobst, G.
A1  - Manz, A.
A1  - Verpoorte, E.
A1  - Widmer, M. W.
A1  - Diamond, D.
A1  - Dempsey, E.
A1  - deViteri, F. J. S.
A1  - Smyth, M.
T1  - Novel instrumentation for real-time monitoring using miniaturized flow systems with integrated biosensors
Y1  - 1997
ER  - 
TY  - JOUR
A1  - Bauer, Christian G.
A1  - Eremenko, A. V.
A1  - Kühn, A.
A1  - Kürzinger, K.
A1  - Markower, Alexander
A1  - Scheller, Frieder W.
T1  - Automated amplifield flow immunoassay for cocaine
Y1  - 1998
ER  - 
TY  - JOUR
A1  - Fridman, Vadim
A1  - Wollenberger, Ursula
A1  - Bogdanovskaya, V. A.
A1  - Lisdat, Fred
A1  - Ruzgas, T.
A1  - Lindgren, A.
A1  - Gorton, Lo
A1  - Scheller, Frieder W.
T1  - Electrochemical investigation of cellobiose oxidation by cellobiose dehydrogenase in the presence of cytochrome c as mediator
Y1  - 2000
ER  - 
TY  - JOUR
A1  - Kapp, A.
A1  - Beissenhirtz, Moritz Karl
A1  - Geyer, F.
A1  - Scheller, F.
A1  - Viezzoli, Maria Silvia
A1  - Lisdat, Fred
T1  - Electrochemical and sensorial behavior of SOD mutants immobilized on gold electrodes in aqueous/organic solvent mixtures
JF  - Electroanalysis : an international journal devoted to fundamental and practical aspects of electroanalysis
N2  - A cysteine mutant of a monomeric human Cu, Zn-SOD (Glycine 61, Serine 142) has been immobilized directly on gold electrodes using the thiol groups introduced. The electrochemical behavior of the surface confined protein was studied in mixtures of aqueous buffer and DMSO up to an organic solvent content of 60%. The formal potential was found to be rather independent of the DMSO content. However, half peak width increased and the redoxactive amount clearly decreased with raising DMSO content. In addition, the kinetics of the heterogeneous electron transfer became slower; but still a quasireversible electrochemical conversion of the mutant SOD was feasible. Thus, the electrodes were applied for sensorial superoxide detection. At a potential of +220 mV vs. Ag/AgCl advantage was taken of the partial oxidation reaction of the enzyme. A defined superoxide signal was obtained in solutions up to 40% DMSO. The sensitivity of the mutant electrodes decreased linearly with the organic solvent content in solution but was still higher compared to conventional cyt.c based sensors. At DMSO concentrations higher than 40% no sensor response was detected.
KW  - SOD
KW  - mutants
KW  - gold electrodes
KW  - DMSO
KW  - electrochemistry
Y1  - 2006
U6  - https://doi.org/10.1002/elan.200603620
SN  - 1040-0397
VL  - 18
SP  - 1909
EP  - 1915
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Ozcelikay, Goksu
A1  - Kurbanoglu, Sevinc
A1  - Zhang, Xiaorong
A1  - Söz, Çağla Kosak
A1  - Wollenberger, Ulla
A1  - Ozkan, Sibel A.
A1  - Yarman, Aysu
A1  - Scheller, Frieder W.
T1  - Electrochemical MIP Sensor for Butyrylcholinesterase
JF  - Polymers
N2  - Molecularly imprinted polymers (MIPs) mimic the binding sites of antibodies by substituting the amino acid-scaffold of proteins by synthetic polymers. In this work, the first MIP for the recognition of the diagnostically relevant enzyme butyrylcholinesterase (BuChE) is presented. The MIP was prepared using electropolymerization of the functional monomer o-phenylenediamine and was deposited as a thin film on a glassy carbon electrode by oxidative potentiodynamic polymerization. Rebinding and removal of the template were detected by cyclic voltammetry using ferricyanide as a redox marker. Furthermore, the enzymatic activity of BuChE rebound to the MIP was measured via the anodic oxidation of thiocholine, the reaction product of butyrylthiocholine. The response was linear between 50 pM and 2 nM concentrations of BuChE with a detection limit of 14.7 pM. In addition to the high sensitivity for BuChE, the sensor responded towards pseudo-irreversible inhibitors in the lower mM range.
KW  - molecularly imprinted polymers
KW  - biomimetic sensors
KW  - butyrylcholinesterase
KW  - o-phenylenediamine
KW  - rivastigmine
Y1  - 2019
U6  - https://doi.org/10.3390/polym11121970
SN  - 2073-4360
VL  - 11
IS  - 12
PB  - MDPI
CY  - Basel
ER  - 
TY  - JOUR
A1  - Bistolas, Nikitas
A1  - Christenson, A.
A1  - Ruzgas, T.
A1  - Jung, Christiane
A1  - Scheller, Frieder W.
A1  - Wollenberger, Ursula
T1  - Spectroelectrochemistry of cytochrome P450cam
N2  - The spectroelectrochemistry of camphor-bound cytochrome P450cam (P450cam) using gold electrodes is described. The electrodes were modified with either 4,4'-dithiodipyridin or sodium dithionite. Electrolysis of P450cam was carried out when the enzyme was in solution, while at the same time UV visible absorption spectra were recorded. Reversible oxidation and reduction could be observed with both 4,4'-dithiodipyridin and dithionite modified electrodes. A formal potential (E-0') of -373 mV vs Ag/AgCl 1 M KCl was determined. The spectra of P450cam complexed with either carbon monoxide or metyrapone, both being inhibitors of P450 catalysis, clearly indicated that the protein retained its native state in the electrochemical cell during electrolysis. (C) 2003 Elsevier Inc. All rights reserved
Y1  - 2004
ER  - 
TY  - JOUR
A1  - Lei, Chenghong
A1  - Wollenberger, Ursula
A1  - Bistolas, Nikitas
A1  - Guiseppi-Eli, A.
A1  - Scheller, Frieder W.
T1  - Electron transfer of hemoglobin at electrodes modified with colloidal clay nanoparticles
Y1  - 2002
ER  -