TY  - GEN
A1  - Wang, Guang
A1  - Li, Pei-zhi
A1  - Zhang, Shi-yao
A1  - Zhong, Shan
A1  - Chu, Chang
A1  - Zeng, Shufei
A1  - Yan, Yu
A1  - Cheng, Xin
A1  - Chuai, Manli
A1  - Hocher, Berthold
A1  - Yang, Xuesong
T1  - Lipopolysaccharides (LPS) Induced Angiogenesis During Chicken Embryogenesis is Abolished by Combined ETA/ETB Receptor Blockade
T2  - Cellular Physiology  and Biochemistry
N2  - Background/Aims: Angiogenesis plays a key role during embryonic development. The vascular endothelin (ET) system is involved in the regulation of angiogenesis. Lipopolysaccharides (LPS) could induce angiogenesis. The effects of ET blockers on baseline and LPS-stimulated angiogenesis during embryonic development remain unknown so far. Methods: The blood vessel density (BVD) of chorioallantoic membranes (CAMs), which were treated with saline (control), LPS, and/or BQ123 and the ETB blocker BQ788, were quantified and analyzed using an IPP 6.0 image analysis program. Moreover, the expressions of ET-1, ET-2, ET3, ET receptor A (ETRA), ET receptor B (ETRB) and VEGFR2 mRNA during embryogenesis were analyzed by semi-quantitative RT-PCR. Results: All components of the ET system are detectable during chicken embryogenesis. LPS increased angiogenesis substantially. This process was completely blocked by the treatment of a combination of the ETA receptor blockers-BQ123 and the ETB receptor blocker BQ788. This effect was accompanied by a decrease in ETRA, ETRB, and VEGFR2 gene expression. However, the baseline angiogenesis was not affected by combined ETA/ETB receptor blockade. Conclusion: During chicken embryogenesis, the LPS-stimulated angiogenesis, but not baseline angiogenesis, is sensitive to combined ETA/ETB receptor blockade. (C) 2018 The Author(s) Published by S. Karger AG, Basel
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 615 
KW  - Lipopolysaccharides  (LPS)
KW  - Angiogenesis
KW  - Chicken  chorioallantoic  membrane  (CAM)
KW  - Endothelin (ET)
Y1  - 2019
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-424552
SN  - 1866-8372
IS  - 615
ER  - 
TY  - JOUR
A1  - Wang, Guang
A1  - Li, Pei-zhi
A1  - Zhang, Shi-yao
A1  - Zhong, Shan
A1  - Chu, Chang
A1  - Zeng, Shufei
A1  - Yan, Yu
A1  - Cheng, Xin
A1  - Chuai, Manli
A1  - Hocher, Berthold
A1  - Yang, Xuesong
T1  - Lipopolysaccharides (LPS) Induced Angiogenesis During Chicken Embryogenesis is Abolished by Combined ETA/ETB Receptor Blockade
JF  - Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry and pharmacology
N2  - Background/Aims: Angiogenesis plays a key role during embryonic development. The vascular endothelin (ET) system is involved in the regulation of angiogenesis. Lipopolysaccharides (LPS) could induce angiogenesis. The effects of ET blockers on baseline and LPS-stimulated angiogenesis during embryonic development remain unknown so far. Methods: The blood vessel density (BVD) of chorioallantoic membranes (CAMs), which were treated with saline (control), LPS, and/or BQ123 and the ETB blocker BQ788, were quantified and analyzed using an IPP 6.0 image analysis program. Moreover, the expressions of ET-1, ET-2, ET3, ET receptor A (ETRA), ET receptor B (ETRB) and VEGFR2 mRNA during embryogenesis were analyzed by semi-quantitative RT-PCR. Results: All components of the ET system are detectable during chicken embryogenesis. LPS increased angiogenesis substantially. This process was completely blocked by the treatment of a combination of the ETA receptor blockers-BQ123 and the ETB receptor blocker BQ788. This effect was accompanied by a decrease in ETRA, ETRB, and VEGFR2 gene expression. However, the baseline angiogenesis was not affected by combined ETA/ETB receptor blockade. Conclusion: During chicken embryogenesis, the LPS-stimulated angiogenesis, but not baseline angiogenesis, is sensitive to combined ETA/ETB receptor blockade.
KW  - Lipopolysaccharides (LPS)
KW  - Angiogenesis
KW  - Chicken chorioallantoic membrane (CAM)
KW  - Endothelin (ET)
Y1  - 2018
U6  - https://doi.org/10.1159/000492547
SN  - 1015-8987
SN  - 1421-9778
VL  - 48
IS  - 5
SP  - 2084
EP  - 2090
PB  - Karger
CY  - Basel
ER  -