TY  - GEN
A1  - Connor, Daniel Oliver
A1  - Zantow, Jonas
A1  - Hust, Michael
A1  - Bier, Frank Fabian
A1  - von Nickisch-Rosenegk, Markus
T1  - Identification of novel immunogenic proteins of Neisseria gonorrhoeae by phage display
T2  - Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe
N2  - Neisseria gonorrhoeae is one of the most prevalent sexually transmitted diseases worldwide with more than 100 million new infections per year. A lack of intense research over the last decades and increasing resistances to the recommended antibiotics call for a better understanding of gonococcal infection, fast diagnostics and therapeutic measures against N. gonorrhoeae. Therefore, the aim of this work was to identify novel immunogenic proteins as a first step to advance those unresolved problems. For the identification of immunogenic proteins, pHORF oligopeptide phage display libraries of the entire N. gonorrhoeae genome were constructed. Several immunogenic oligopeptides were identified using polyclonal rabbit antibodies against N. gonorrhoeae. Corresponding full-length proteins of the identified oligopeptides were expressed and their immunogenic character was verified by ELISA. The immunogenic character of six proteins was identified for the first time. Additional 13 proteins were verified as immunogenic proteins in N. gonorrhoeae.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 541 
KW  - proteomic analysis
KW  - vaccine antigens
KW  - gene-expression
KW  - Mycobacterium tuberculosis
KW  - antimicrobial resistance
KW  - recombinant antibodies
KW  - Salmonella Thyphimurium
KW  - untreatable Gonorrhea
KW  - multidrug-resistant
KW  - Escherichia coli
Y1  - 2019
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-411077
SN  - 1866-8372
IS  - 541
ER  -