TY  - GEN
A1  - Jantzen, Friederike
A1  - Wozniak, Natalia Joanna
A1  - Kappel, Christian
A1  - Sicard, Adrien
A1  - Lenhard, Michael
T1  - A high‑throughput amplicon‑based method for estimating outcrossing rates
T2  - Postprints der Universität Potsdam Mathematisch-Naturwissenschaftliche Reihe
N2  - Background: The outcrossing rate is a key determinant of the population-genetic structure of species and their long-term evolutionary trajectories. However, determining the outcrossing rate using current methods based on PCRgenotyping individual offspring of focal plants for multiple polymorphic markers is laborious and time-consuming.

Results: We have developed an amplicon-based, high-throughput enabled method for estimating the outcrossing rate and have applied this to an example of scented versus non-scented Capsella (Shepherd’s Purse) genotypes. Our results show that the method is able to robustly capture differences in outcrossing rates. They also highlight potential biases in the estimates resulting from differential haplotype sharing of the focal plants with the pollen-donor population at individual amplicons.

Conclusions: This novel method for estimating outcrossing rates will allow determining this key population-genetic parameter with high-throughput across many genotypes in a population, enabling studies into the genetic determinants of successful pollinator attraction and outcrossing.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 745 
KW  - Outcrossing
KW  - Mixed mating
KW  - Outcrossing rate
KW  - Capsella
KW  - Amplicon sequencing
Y1  - 2019
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-435657
SN  - 1866-8372
IS  - 745
ER  - 
TY  - JOUR
A1  - Jantzen, Friederike
A1  - Wozniak, Natalia Joanna
A1  - Kappel, Christian
A1  - Sicard, Adrien
A1  - Lenhard, Michael
T1  - A high‑throughput amplicon‑based method for estimating outcrossing rates
JF  - Plant Methods
N2  - Background: The outcrossing rate is a key determinant of the population-genetic structure of species and their long-term evolutionary trajectories. However, determining the outcrossing rate using current methods based on PCRgenotyping individual offspring of focal plants for multiple polymorphic markers is laborious and time-consuming.

Results: We have developed an amplicon-based, high-throughput enabled method for estimating the outcrossing rate and have applied this to an example of scented versus non-scented Capsella (Shepherd’s Purse) genotypes. Our results show that the method is able to robustly capture differences in outcrossing rates. They also highlight potential biases in the estimates resulting from differential haplotype sharing of the focal plants with the pollen-donor population at individual amplicons.

Conclusions: This novel method for estimating outcrossing rates will allow determining this key population-genetic parameter with high-throughput across many genotypes in a population, enabling studies into the genetic determinants of successful pollinator attraction and outcrossing.
KW  - Outcrossing
KW  - Mixed mating
KW  - Outcrossing rate
KW  - Capsella
KW  - Amplicon sequencing
Y1  - 2019
U6  - https://doi.org/10.1186/s13007-019-0433-9
SN  - 1746-4811
VL  - 15
IS  - 47
PB  - BioMed Central
CY  - London
ER  -